Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,601,276	G→A	P271S (CCG→TCG)	ftsX ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,601,276	0	G	A	100.0%	162.6 / NA	42	P271S (CCG→TCG)	ftsX	putative ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/17); total (25/17)

ATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACG  >  NC_000913/3601129‑3601418

aTGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAgcg                                                                                                                                             >  1:223243/1‑151 (MQ=255)

  gATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAgcgc                                                                                                                                            >  2:567816/1‑150 (MQ=255)

        ccATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGCTGACAATCGCAGCACCAGAATTTCTGACAACATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATg                                                                                                                                      >  1:88082/1‑150 (MQ=255)

            tGATCTCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATcc                                                                                                                                 >  1:163565/1‑151 (MQ=255)

                       ttCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCAc                                                                                                                      >  1:655158/1‑151 (MQ=255)

                               aaaacCCTGTCCCCCTCCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCg                                                                                                                         <  1:653879/139‑1 (MQ=255)

                                aaacccggccccACTTCGGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAAt                                                                                                                  <  1:644798/136‑1 (MQ=255)

                                aaacCCTGGCCCCCTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCa                                                                                                                <  2:481398/148‑1 (MQ=255)

                                aaaaCCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGtt                                                                                                             >  1:334486/1‑151 (MQ=255)

                                           ctctaccGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGtt                                                                                                   <  1:396720/145‑1 (MQ=255)

                                             ctcccGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGt                                                                                                        <  2:463998/140‑1 (MQ=255)

                                             cTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAAt                                                                                                >  2:143709/1‑151 (MQ=255)

                                              ttCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTa                                                                                                  >  2:234644/1‑148 (MQ=255)

                                                    aaCCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTc                                                                                          >  1:488701/1‑150 (MQ=255)

                                                          cgATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCg                                                                                    >  1:376908/1‑150 (MQ=255)

                                                           gATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCg                                                                                    >  2:278204/1‑149 (MQ=255)

                                                                 aaTCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCTCCATACAGGAACGAGCGCAGGATGAAGCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGGGCaac                                                                              <  1:173798/149‑2 (MQ=255)

                                                                 aaTCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCaa                                                                               >  2:646387/1‑148 (MQ=255)

                                                                    cGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATAc                                                                         <  2:16225/151‑1 (MQ=255)

                                                                     gcagcaCCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACt                                                                        >  1:593162/1‑151 (MQ=255)

                                                                         caCCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTc                                                                       >  1:333123/1‑148 (MQ=255)

                                                                           ccAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGAca                                                                  <  1:146974/151‑2 (MQ=255)

                                                                               aaTTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGcaca                                                              <  1:546272/151‑1 (MQ=255)

                                                                                aTTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCacac                                                             >  1:339583/1‑151 (MQ=255)

                                                                                  ttCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTg                                                           >  1:633338/1‑151 (MQ=255)

                                                                                       aCAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTAcc                                                      >  1:376083/1‑151 (MQ=255)

                                                                                                   acaacaATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGaa                                          <  2:593167/151‑1 (MQ=255)

                                                                                                        aaTGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACgg                                     >  2:27420/1‑151 (MQ=255)

                                                                                                                     aTCCCAGCAGTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCa                         >  2:439382/1‑150 (MQ=255)

                                                                                                                         cagcagTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACg                    <  1:298419/151‑1 (MQ=255)

                                                                                                                          agcagTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGc                   <  2:345229/151‑1 (MQ=255)

                                                                                                                             agTGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGa                <  1:381369/151‑1 (MQ=255)

                                                                                                                               tGCGCCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGatc              >  1:479568/1‑151 (MQ=255)

                                                                                                                                 cgcCACCATACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGatcat            <  2:389252/151‑1 (MQ=255)

                                                                                                                                   ccaccaTACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCg          >  2:8557/1‑151 (MQ=255)

                                                                                                                                    caccaTACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCAgcgc         <  1:228272/151‑1 (MQ=255)

                                                                                                                                      ccaTACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCg       >  2:387374/1‑151 (MQ=255)

                                                                                                                                      ccaTACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCg       >  2:656149/1‑151 (MQ=255)

                                                                                                                                       caTACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCg       >  2:434578/1‑150 (MQ=255)

                                                                                                                                        aTACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGaa     <  1:665413/151‑1 (MQ=255)

                                                                                                                                         tACAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGaa     >  1:605223/1‑150 (MQ=255)

                                                                                                                                           cAGGAACGAGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAAcg  <  1:278204/151‑1 (MQ=255)

ATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGTACGTTAATGGAGTCACGGCGAGCAAAGATACTCAGACGCACACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACG  >  NC_000913/3601129‑3601418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A