Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,893,403	C→T	S213S (AGC→AGT)	yidZ →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,893,403	0	C	T	100.0%	153.2 / NA	40	S213S (AGC→AGT)	yidZ	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/20); total (20/20)

TCGCTACCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGCGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAGCGCAACCCGACAATCTGCTACTGGCGACCGCGCCGCGCTACT  >  NC_000913/3893257‑3893542

tCGCTACCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGAt                                                                                                                                          >  1:216702/1‑150 (MQ=255)

    tACCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTg                                                                                                                                     >  2:7035/1‑151 (MQ=255)

     aCCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACTCCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCt                                                                                                                                      <  1:524360/149‑1 (MQ=255)

     aCCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCt                                                                                                                                      <  1:10881/149‑1 (MQ=255)

      ccGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTggg                                                                                                                                   >  2:153873/1‑151 (MQ=255)

          tAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCt                                                                                                                               >  2:431651/1‑151 (MQ=255)

                  gATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAAtg                                                                                                                       <  1:396276/151‑1 (MQ=255)

                  gATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAAt                                                                                                                        <  2:190068/150‑1 (MQ=255)

                             gCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGa                                                                                                             <  1:315326/150‑1 (MQ=255)

                                    aGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGAc                                                                                                     >  1:171191/1‑151 (MQ=255)

                                       gATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGAcgcg                                                                                                  >  1:255707/1‑151 (MQ=255)

                                          gtgCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAAc                                                                                               <  2:28896/151‑1 (MQ=255)

                                            gCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACg                                                                                              <  1:444684/150‑1 (MQ=255)

                                            gCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGc                                                                                             >  1:131047/1‑151 (MQ=255)

                                            gCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGc                                                                                             >  1:237067/1‑151 (MQ=255)

                                                tGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACg                                                                                          >  1:430721/1‑150 (MQ=255)

                                                        gTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATg                                                                                 <  2:583595/151‑1 (MQ=255)

                                                               aaaGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGc                                                                          >  1:379470/1‑151 (MQ=255)

                                                                        ccGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTc                                                                  <  1:535003/150‑1 (MQ=255)

                                                                        ccGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTc                                                                  <  1:7036/150‑1 (MQ=255)

                                                                        ccGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCg                                                                 <  1:218643/151‑1 (MQ=255)

                                                                            cACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCa                                                             <  1:99201/151‑1 (MQ=255)

                                                                                gCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAg                                                            >  2:445765/1‑148 (MQ=255)

                                                                                gCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTc                                                          <  1:375975/150‑1 (MQ=255)

                                                                                gCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTc                                                          >  1:554461/1‑150 (MQ=255)

                                                                                 cATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCAc                                                        >  2:299165/1‑151 (MQ=255)

                                                                                        cGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTAt                                                 <  2:444432/151‑1 (MQ=255)

                                                                                         gTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATg                                                <  2:139999/151‑1 (MQ=255)

                                                                                            gAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCa                                             <  2:404825/151‑1 (MQ=255)

                                                                                             aaTCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGc                                              >  2:139856/1‑149 (MQ=255)

                                                                                                cTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAgcgc                                         >  2:620942/1‑151 (MQ=255)

                                                                                                      acCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGACCAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCTCTGTTTATGGCAGCGCAAccc                                    <  1:68953/150‑1 (MQ=255)

                                                                                                                   aTCCGCATATCAGCATTTGCTGGGAACAGAGTGATACCTAGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAGCGCAACCCGACAATCTGCTAc                       <  1:619688/150‑1 (MQ=255)

                                                                                                                     ccGCATATCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAGCGCAACCCGACAATCTGCTACTgg                    >  1:522450/1‑151 (MQ=255)

                                                                                                                         atatCAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAGCGCAACCCGACAATCTGCTACTGGCGa                 <  1:364408/150‑1 (MQ=255)

                                                                                                                             cAGCATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAGCGCAACCCGACAATCTGCTACTGGCGAccgc             <  1:456007/150‑1 (MQ=255)

                                                                                                                               gCATTTGCTGGGAGCAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAGCGCAACCCGACAATCTGCTACTGGCGAccgcgcc          >  1:21648/1‑151 (MQ=255)

                                                                                                                                cATTTGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAGCGCAACCCGACAATCTGCTACTGGCGAccgcgcc          >  1:357343/1‑150 (MQ=255)

                                                                                                                                    tGCTGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAGCGCAACCCGACAATCTGCTACTGGCGACCGCGCCGCGCt     >  1:521423/1‑151 (MQ=255)

                                                                                                                                       tGGGAACAGAGTGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAGCGCAACCCGACAATCTGCTACTGGCGACCGCGCCGCGCTACt  >  2:602667/1‑151 (MQ=255)

TCGCTACCGTTAGCCATTGATTATGAAGTGCTGTTTAGTGATGTGCCCTGCGTCTGGTTACGCAAAGATCATCCGGCACTGCATCAAACGTGGAATCTGGACACCTTCTTACGTTATCCGCATATCAGCATTTGCTGGGAACAGAGCGATACCTGGGCGCTGGACAATGTGTTACAGGAGCTGGGACGCGAACGCACGATTGCTATGAGCCTGCCGGAATTCGAGCAGTCACTGTTTATGGCAGCGCAACCCGACAATCTGCTACTGGCGACCGCGCCGCGCTACT  >  NC_000913/3893257‑3893542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A