Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,967,093	A→G	E226G (GAA→GGA)	rho →	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,967,093	0	A	G	100.0%	134.5 / NA	38	E226G (GAA→GGA)	rho	transcription termination factor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/16); total (22/16)

GGTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTT  >  NC_000913/3966963‑3967236

ggTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTg                                                                                                                             <  2:46790/151‑1 (MQ=255)

     aaCCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGaa                                                                                                                        <  1:382678/151‑1 (MQ=255)

         aTGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAAccc                                                                                                                     >  2:513934/1‑150 (MQ=255)

           gctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCa                                                                                                                  <  1:71151/151‑1 (MQ=255)

              gctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCAtc                                                                                                                <  2:9991/150‑1 (MQ=255)

                tgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCg                                                                                                             >  1:36240/1‑151 (MQ=255)

                   aGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGcc                                                                                                           >  2:536409/1‑150 (MQ=255)

                          tGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCa                                                                                                    <  2:89617/150‑1 (MQ=255)

                           gCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGCACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAgg                                                                                                  >  2:509230/1‑151 (MQ=255)

                            cTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGt                                                                                                 >  1:5138/1‑151 (MQ=255)

                            cTCAGAGCATTGCTTACAACCACCCGGATTGGGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCAAGATGCAGCGTATGGTAAAAGGTGGAGGTGTTGCGTCTACCTTTGACGAACCCGCATCTAGCCACGTTCCGGt                                                                                                 >  1:318921/1‑151 (MQ=255)

                                gagCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCg                                                                                             >  2:313997/1‑151 (MQ=255)

                                    aTTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGaa                                                                                          >  1:380033/1‑150 (MQ=255)

                                       gCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATgg                                                                                      <  1:654458/151‑1 (MQ=255)

                                       gCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATgg                                                                                      <  2:118518/151‑1 (MQ=255)

                                        cTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGt                                                                                     >  2:249381/1‑151 (MQ=255)

                                           aCAACCACCCGGATTGTGTGCTGATGGTTCCGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGAt                                                                                  >  2:14733/1‑151 (MQ=255)

                                                  cccGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCgaga                                                                             >  1:471363/1‑149 (MQ=255)

                                                       aTTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCaa                                                                      <  1:536405/151‑1 (MQ=255)

                                                           tgtgCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGc                                                                  >  2:299356/1‑151 (MQ=255)

                                                              gCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCt                                                                <  1:39698/150‑1 (MQ=255)

                                                              gCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCt                                                                <  2:62101/150‑1 (MQ=255)

                                                              gCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCt                                                                <  1:506144/150‑1 (MQ=255)

                                                                   tGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTg                                                           >  1:13874/1‑150 (MQ=255)

                                                                     gTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGc                                                        >  2:479438/1‑151 (MQ=255)

                                                                          gctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACaa                                                    <  1:44540/150‑1 (MQ=255)

                                                                                        gTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCAt                                     >  1:344841/1‑151 (MQ=255)

                                                                                        gTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGGGATCGAGAAGGCCAAACGCCTCGTTGAGCACAAGACAGACGTTATCAt                                     >  1:17918/1‑151 (MQ=255)

                                                                                         tCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCAtt                                    >  2:48617/1‑151 (MQ=255)

                                                                                            ggAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTg                                 <  1:70392/151‑1 (MQ=255)

                                                                                                    tAACCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTc                         >  2:455081/1‑151 (MQ=255)

                                                                                                     aaCCGAGATGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTcc                        >  1:53869/1‑151 (MQ=255)

                                                                                                          agaTGCAGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCAc                   <  2:418389/151‑1 (MQ=255)

                                                                                                                aGCGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAAAGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCt             >  1:148664/1‑151 (MQ=255)

                                                                                                                  cGTCTGGTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCt             >  1:261523/1‑149 (MQ=255)

                                                                                                                       ggTAAAAGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGgcgcg       <  2:344844/150‑1 (MQ=255)

                                                                                                                           aaaGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCtt  <  1:457411/151‑1 (MQ=255)

                                                                                                                            aaGGTGGAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCtt  >  1:449105/1‑150 (MQ=255)

GGTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTT  >  NC_000913/3966963‑3967236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A