Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,102,930	C→T	L37L (CTG→TTG)	yiiM →	6‑N‑hydroxylaminopurine resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,102,930	0	C	T	100.0%	125.1 / NA	32	L37L (CTG→TTG)	yiiM	6‑N‑hydroxylaminopurine resistance protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/15); total (17/15)

AATGGGGAAATTTCTCGTGGAGAGGGAACAGATGCGATATCCGGTTGATGTATACACAGGCAAGATTCAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTG  >  NC_000913/4102791‑4103077

aaTGGGGAAATTTCTCGTGGAGAGGGAACAGATGCGATATCCGGTTGATGTATACACAGGCAAGATTCAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGa                                                                                                                                           >  2:103022/1‑150 (MQ=255)

      gAAATTTCTCGTGGAGAGGGAACAGATGCGATATCCGGTTGATGTATACACAGGCAAGATTCAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGAc                                                                                                                                    <  2:70949/151‑1 (MQ=255)

               cGTGGAGAGGGAACAGATGCGATATCCGGTTGATGTATACACAGGCAAGATTCAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGc                                                                                                                            <  2:388006/150‑1 (MQ=255)

                  ggAGAGGGAACAGATGCGATATCCGGTTGATGTATACACAGGCAAGATTCAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCgg                                                                                                                          >  1:10883/1‑149 (MQ=255)

                                tGCGATATCCGGTTGATGTATACACAGGCAAGATTCAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCAcgg                                                                                                          >  2:32689/1‑151 (MQ=255)

                                         cGGTTGATGTATACACAGGCAAGATTCAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGcc                                                                                                    >  2:458701/1‑148 (MQ=255)

                                             tGATGTATACACAGGCAAGATTCAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCcagacag                                                                                              <  2:569457/150‑1 (MQ=255)

                                              gATGTATACACAGGCAAGATTCAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCcagacaga                                                                                             <  1:228569/150‑1 (MQ=255)

                                                                   cAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTg                                                                       <  1:53278/151‑1 (MQ=255)

                                                                         tATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCa                                                                   <  1:244384/149‑1 (MQ=255)

                                                                            cccGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTAt                                                               <  1:298526/150‑1 (MQ=255)

                                                                            cccGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTAt                                                               >  2:459676/1‑150 (MQ=255)

                                                                             ccGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTAtct                                                             <  2:492557/151‑1 (MQ=255)

                                                                             ccGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTAtct                                                             >  2:350472/1‑151 (MQ=255)

                                                                                aaGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTAtctc                                                            >  1:6665/1‑149 (MQ=255)

                                                                                  ggCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACt                                                        >  2:380739/1‑151 (MQ=255)

                                                                                   gCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTg                                                       >  1:619841/1‑151 (MQ=255)

                                                                                      aaCCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTggg                                                     >  2:100167/1‑150 (MQ=255)

                                                                                              gcAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAAt                                            >  1:157332/1‑151 (MQ=255)

                                                                                              gcAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAAt                                            <  1:578610/151‑1 (MQ=255)

                                                                                               cAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAAtt                                           >  2:552165/1‑151 (MQ=255)

                                                                                                aaTTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAAttt                                          <  2:418622/151‑1 (MQ=255)

                                                                                                   tGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAAttttc                                        >  1:635613/1‑148 (MQ=255)

                                                                                                       aaaaTCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGaa                                    >  1:646599/1‑150 (MQ=255)

                                                                                                       aaaaTCCAGGTTGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCAACTGGGCGCGGGAATTTCCGGa                                     <  1:476298/149‑1 (MQ=255)

                                                                                                                  tGATGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAgt                          >  1:2633/1‑149 (MQ=255)

                                                                                                                     tGGTGAGTTGATGCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGGGttgttt                     >  1:291746/1‑151 (MQ=255)

                                                                                                                                 gCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGc          <  1:457771/150‑1 (MQ=255)

                                                                                                                                 gCTGACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGc          <  1:409258/150‑1 (MQ=255)

                                                                                                                                    gACGGAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGtt       <  2:618552/150‑1 (MQ=255)

                                                                                                                                       ggAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTgg    >  2:240225/1‑150 (MQ=255)

                                                                                                                                        gAGTTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTg  <  2:75005/151‑1 (MQ=255)

AATGGGGAAATTTCTCGTGGAGAGGGAACAGATGCGATATCCGGTTGATGTATACACAGGCAAGATTCAGGCTTATCCCGAAGGCAAACCCAGCGCAATTGCTAAAATCCAGGTTGATGGTGAGTTGATGCTGACGGAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTG  >  NC_000913/4102791‑4103077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A