Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,143,617	A→G	intergenic (+8/‑7)	frwC → / → frwB	putative enzyme IIC component of PTS/putative enzyme IIB component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,143,617	0	A	G	100.0%	135.4 / NA	38	intergenic (+8/‑7)	frwC/frwB	putative enzyme IIC component of PTS/putative enzyme IIB component of PTS
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/19); total (19/19)

GTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAA  >  NC_000913/4143473‑4143762

gTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGcc                                                                                                                                             <  2:269840/151‑1 (MQ=255)

gTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGcc                                                                                                                                             <  2:638346/151‑1 (MQ=255)

   atatCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCAt                                                                                                                                           <  1:150198/150‑1 (MQ=255)

   atatCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATg                                                                                                                                          >  2:37765/1‑151 (MQ=255)

   atatCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAAAGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCAt                                                                                                                                           >  1:398569/1‑150 (MQ=255)

         cagcagTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGaa                                                                                                                                     >  1:206743/1‑150 (MQ=255)

                ggCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAattatt                                                                                                                              <  2:28330/150‑1 (MQ=255)

                ggCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAattatt                                                                                                                              <  2:485351/150‑1 (MQ=255)

                    gTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCa                                                                                                                           <  1:425476/149‑1 (MQ=255)

                                gtgACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTcc                                                                                                             >  2:129203/1‑151 (MQ=255)

                                  gACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCtt                                                                                                           <  2:323291/151‑1 (MQ=255)

                                    cGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTc                                                                                                          >  1:452519/1‑150 (MQ=255)

                                        tGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGgtg                                                                                                     >  2:464673/1‑151 (MQ=255)

                                         gTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGgt                                                                                                      <  2:417257/149‑1 (MQ=255)

                                         gTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGgt                                                                                                      <  1:371777/149‑1 (MQ=255)

                                                 gAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCAt                                                                                             <  1:639309/150‑1 (MQ=255)

                                                 gAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCAt                                                                                             <  1:102192/150‑1 (MQ=255)

                                                         tGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATg                                                                                    >  1:474475/1‑151 (MQ=255)

                                                              aGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGgcagc                                                                               <  2:594678/151‑1 (MQ=255)

                                                               gTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGgcagca                                                                              <  1:658835/151‑1 (MQ=255)

                                                                          aaaaaTGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTgg                                                                    >  1:59805/1‑150 (MQ=255)

                                                                            aaaTGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGaaa                                                                 <  2:645646/151‑1 (MQ=255)

                                                                            aaaTGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGaaa                                                                 <  1:134390/151‑1 (MQ=255)

                                                                            aaaTGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGaa                                                                  >  1:509420/1‑150 (MQ=255)

                                                                              aTGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGt                                                               >  1:270808/1‑151 (MQ=255)

                                                                                      tCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGGGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCgcga                                                        >  1:536268/1‑150 (MQ=255)

                                                                                         aCTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCgcgaaagc                                                    <  2:410740/151‑1 (MQ=255)

                                                                                                aaaaaGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGc                                             >  1:51089/1‑151 (MQ=255)

                                                                                                  aaaGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTg                                           >  2:480884/1‑151 (MQ=255)

                                                                                                           gacCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGaa                                  <  2:51088/151‑1 (MQ=255)

                                                                                                            acCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGaa                                  >  1:232895/1‑150 (MQ=255)

                                                                                                               tggatttggattTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGt                               >  2:285087/1‑150 (MQ=255)

                                                                                                                    ttggatttTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAAc                         >  2:212712/1‑151 (MQ=255)

                                                                                                                    ttggatttTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAAc                         >  1:336046/1‑151 (MQ=255)

                                                                                                                              aaaTTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCa               <  2:231396/151‑1 (MQ=255)

                                                                                                                                      taattGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCt       >  1:304218/1‑151 (MQ=255)

                                                                                                                                          tGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGa    <  2:270808/150‑1 (MQ=255)

                                                                                                                                            aGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGaaa  >  2:381017/1‑150 (MQ=255)

GTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAA  >  NC_000913/4143473‑4143762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A