Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,154,549	T→C	E100G (GAG→GGG)	argE ←	acetylornithine deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,154,549	0	T	C	100.0%	145.5 / NA	40	E100G (GAG→GGG)	argE	acetylornithine deacetylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/17); total (23/17)

GCCATACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGC  >  NC_000913/4154402‑4154689

gCCATACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCg                                                                                                                                           >  1:393111/1‑151 (MQ=255)

    tACTGGTTTCTTCATCAGCAGTCGCCAGCATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGCCATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTc                                                                                                                                         >  2:454314/1‑149 (MQ=255)

    tACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTc                                                                                                                                         >  2:530662/1‑149 (MQ=255)

           ttcttcATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTaaa                                                                                                                                 <  2:309120/150‑1 (MQ=255)

           ttcttcATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAAc                                                                                                                                >  2:300315/1‑151 (MQ=255)

               tcatcaGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACg                                                                                                                               <  2:200945/148‑1 (MQ=255)

               tcatcaGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGAt                                                                                                                            >  2:3836/1‑151 (MQ=255)

                catcaGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGAt                                                                                                                            <  1:524028/150‑1 (MQ=255)

                              aGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGAc                                                                                                             <  1:630889/151‑1 (MQ=255)

                                 aTGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCg                                                                                                           >  2:169574/1‑150 (MQ=255)

                                   gTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGtc                                                                                                         <  2:75868/150‑1 (MQ=255)

                                        gCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCaaa                                                                                                   <  2:145463/151‑1 (MQ=255)

                                               tttCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAGCGGATCGCGCGTCCAGCGACCGTCATCAAATGGCAc                                                                                             <  2:150729/150‑1 (MQ=255)

                                                           gACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGc                                                                                >  2:6392/1‑151 (MQ=255)

                                                             cGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGccc                                                                              >  1:567057/1‑151 (MQ=255)

                                                                     tCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGc                                                                       >  2:301549/1‑150 (MQ=255)

                                                                      cgcgTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGca                                                                      <  1:41073/150‑1 (MQ=255)

                                                                         gTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGcaacaa                                                                  <  2:325784/151‑1 (MQ=255)

                                                                         gTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGcaacaa                                                                  <  1:596006/151‑1 (MQ=255)

                                                                            gcgcATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAg                                                                 <  2:552384/149‑1 (MQ=255)

                                                                                  cAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGc                                                         >  1:201357/1‑151 (MQ=255)

                                                                                    aGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGccc                                                       >  1:259016/1‑151 (MQ=255)

                                                                                          aaCGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTc                                                 >  1:190578/1‑151 (MQ=255)

                                                                                            cGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCg                                               >  2:283349/1‑151 (MQ=255)

                                                                                                aaaaaGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATAc                                           >  2:29887/1‑151 (MQ=255)

                                                                                                 aaaaGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACt                                          >  1:478345/1‑151 (MQ=255)

                                                                                                   aaGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCGTGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTg                                        <  2:181283/151‑1 (MQ=255)

                                                                                                             tGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATAtt                              >  2:13112/1‑151 (MQ=255)

                                                                                                              gTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATAtt                              >  2:277793/1‑150 (MQ=255)

                                                                                                                        ccTAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGc                   >  2:35147/1‑151 (MQ=255)

                                                                                                                          tAAGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCg                  >  1:570056/1‑150 (MQ=255)

                                                                                                                           aaGCCGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAg                <  1:530659/151‑1 (MQ=255)

                                                                                                                              ccGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTc             <  2:38944/151‑1 (MQ=255)

                                                                                                                               cGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTg                                                   >  2:301355/1‑112 (MQ=255)

                                                                                                                               cGTAAAGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTcc            >  1:27136/1‑151 (MQ=255)

                                                                                                                                  aaaGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGCCCAGCGACCGTCATCAAAGGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTg                                                   <  1:301353/109‑1 (MQ=255)

                                                                                                                                   aaGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATTCTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGa        <  2:393115/151‑1 (MQ=255)

                                                                                                                                   aaGCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGa        <  2:77509/151‑1 (MQ=255)

                                                                                                                                     gCTTGCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAAc      >  1:71048/1‑151 (MQ=255)

                                                                                                                                         gCCGTCATGCCCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGc  >  2:594310/1‑151 (MQ=255)

GCCATACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGC  >  NC_000913/4154402‑4154689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A