Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,356,166	C→T	A82A (GCG→GCA)	dtpC ←	dipeptide and tripeptide permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,356,166	0	C	T	100.0%	133.3 / NA	36	A82A (GCG→GCA)	dtpC	dipeptide and tripeptide permease
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/17); total (19/17)

CGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGCGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGATGG  >  NC_000913/4356018‑4356304

cGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAgtgc                                                                                                                                          >  2:116434/1‑151 (MQ=255)

            agagCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCa                                                                                                                               <  2:488000/150‑1 (MQ=255)

            agagCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCa                                                                                                                               <  1:605103/150‑1 (MQ=255)

             gagCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACt                                                                                                                             <  1:192851/151‑1 (MQ=255)

                   gCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTg                                                                                                                       >  2:36083/1‑151 (MQ=255)

                           aaCAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGcc                                                                                                               >  1:527801/1‑151 (MQ=255)

                            aCAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCGGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCg                                                                                                              <  2:89844/151‑1 (MQ=255)

                               gCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGc                                                                                                           >  2:306402/1‑151 (MQ=255)

                                 tGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGc                                                                                                           <  1:659092/149‑1 (MQ=255)

                                    tGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCg                                                                                                      >  2:213872/1‑151 (MQ=255)

                                       ttgatttgaATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTc                                                                                                   >  2:420653/1‑151 (MQ=255)

                                        tgatttgaATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTc                                                                                                   <  2:349589/150‑1 (MQ=255)

                                          atttgaATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGc                                                                                                >  2:77629/1‑151 (MQ=255)

                                             tgaATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGc                                                                                                >  1:468560/1‑148 (MQ=255)

                                                    aCCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAgccg                                                                                      <  1:197667/151‑1 (MQ=255)

                                                       gTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGccgccg                                                                                   <  1:36084/151‑1 (MQ=255)

                                                                    atGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTaa                                                                      <  2:215749/151‑1 (MQ=255)

                                                                      gATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACg                                                                    >  2:570285/1‑151 (MQ=255)

                                                                      gATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACg                                                                    >  2:195908/1‑151 (MQ=255)

                                                                           ccAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAAc                                                               <  2:373536/151‑1 (MQ=255)

                                                                                gcCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCagag                                                          <  1:314553/151‑1 (MQ=255)

                                                                                  cAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAgaga                                                         >  1:448432/1‑150 (MQ=255)

                                                                                  cAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAgaga                                                         >  1:63326/1‑150 (MQ=255)

                                                                                     aTACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGc                                                      >  2:618046/1‑150 (MQ=255)

                                                                                         aGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATg                                                 >  2:177436/1‑151 (MQ=255)

                                                                                          ggCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATgc                                                >  2:405258/1‑151 (MQ=255)

                                                                                              aaaGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTg                                            <  2:643204/151‑1 (MQ=255)

                                                                                                  gTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACa                                        <  2:179917/151‑1 (MQ=255)

                                                                                                   ttGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACa                                        <  1:213871/150‑1 (MQ=255)

                                                                                                         ttGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGAt                                 >  2:55765/1‑151 (MQ=255)

                                                                                                             aTCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGc                              <  1:147150/150‑1 (MQ=255)

                                                                                                             aTCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGc                              <  1:258789/150‑1 (MQ=255)

                                                                                                                    cccAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGtt                        <  1:32669/149‑1 (MQ=255)

                                                                                                                        gCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTatcatc                  >  1:62542/1‑151 (MQ=255)

                                                                                                                                    cAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGTGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTg      >  2:575266/1‑151 (MQ=255)

                                                                                                                                      aGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGa     >  1:536748/1‑150 (MQ=255)

                                                                                                                                        ggTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGATgg  >  2:26304/1‑151 (MQ=255)

CGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGCGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGATGG  >  NC_000913/4356018‑4356304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A