Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,688,959	A→G	V183A (GTT→GCT)	yfhG ←	putative outer membrane protein modulating the QseEF response

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,688,959	0	A	G	100.0%	148.9 / NA	38	V183A (GTT→GCT)	yfhG	putative outer membrane protein modulating the QseEF response
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/17); total (21/17)

GGATGGCGCGGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAACGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAACCTGTGCCGGGATC  >  NC_000913/2688823‑2689105

ggATGGCGCGGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTgg                                                                                                                                      <  1:575280/151‑1 (MQ=255)

 gATGGCGCGGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGgc                                                                                                                                     <  2:389996/151‑1 (MQ=255)

    ggCGCGGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCa                                                                                                                                  <  2:69370/151‑1 (MQ=255)

        cgGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGt                                                                                                                              >  2:368959/1‑151 (MQ=255)

           gCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGtctc                                                                                                                           >  1:41340/1‑151 (MQ=255)

               ttCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAg                                                                                                                        <  2:50103/150‑1 (MQ=255)

                    tctcGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCtcgct                                                                                                                  >  2:308598/1‑151 (MQ=255)

                           cggcgTAGCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCtcgctgtcgctg                                                                                                           <  1:69381/151‑1 (MQ=255)

                              cgTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGAc                                                                                                        <  1:125757/151‑1 (MQ=255)

                               gTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGAct                                                                                                       <  2:251398/151‑1 (MQ=255)

                                 aTCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGActgc                                                                                                     >  2:67512/1‑151 (MQ=255)

                                 aTCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGActg                                                                                                      <  2:267847/150‑1 (MQ=255)

                                  tCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGActgct                                                                                                    >  2:53938/1‑151 (MQ=255)

                                    cGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTgg                                                                                                  >  2:306667/1‑151 (MQ=255)

                                        gagaAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGtt                                                                                              <  2:5055/151‑1 (MQ=255)

                                            aaTTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACt                                                                                          >  1:422496/1‑151 (MQ=255)

                                             aTTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTg                                                                                         <  2:201108/151‑1 (MQ=255)

                                                       ggTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGGCTGCTGGAGTTTACTGTAACGTTGgc                                                                               >  1:179295/1‑151 (MQ=255)

                                                                   gagaGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGcc                                                                    >  2:327427/1‑150 (MQ=255)

                                                                           gCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCcagttgcagt                                                           >  2:630974/1‑151 (MQ=255)

                                                                                 aaTATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAgc                                                     >  2:301318/1‑151 (MQ=255)

                                                                                   tatCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAgcgc                                                   >  2:121844/1‑151 (MQ=255)

                                                                                   tatCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAgc                                                     >  1:403635/1‑149 (MQ=255)

                                                                                    atCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGcc                                                  <  1:12257/151‑1 (MQ=255)

                                                                                      cGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCt                                                 <  2:528724/150‑1 (MQ=255)

                                                                                      cGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTg                                                >  2:157269/1‑151 (MQ=255)

                                                                                          cAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGAcc                                             <  1:586672/150‑1 (MQ=255)

                                                                                             gTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATcg                                         <  2:175622/151‑1 (MQ=255)

                                                                                                        tGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGtt                               <  1:458575/150‑1 (MQ=255)

                                                                                                         gCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTg                              >  1:399035/1‑150 (MQ=255)

                                                                                                          cGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGAt                            >  1:290176/1‑151 (MQ=255)

                                                                                                                   aGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGc                   >  1:166675/1‑151 (MQ=255)

                                                                                                                     ttCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCg                  >  1:227388/1‑150 (MQ=255)

                                                                                                                      tCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGa                 <  1:351962/150‑1 (MQ=255)

                                                                                                                        cAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTAGCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTGGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAAcc              <  1:138721/151‑1 (MQ=255)

                                                                                                                               tgctgTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAACCTGTGCCg       >  2:141987/1‑151 (MQ=255)

                                                                                                                                    tAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAACCTGTGCCGGGAtc  >  2:375963/1‑151 (MQ=255)

                                                                                                                                    tAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAACCTGTGCCGGGAtc  >  2:433372/1‑151 (MQ=255)

GGATGGCGCGGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAACGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAACCTGTGCCGGGATC  >  NC_000913/2688823‑2689105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A