Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F50 I2 R1 104 44.1 1376607 98.9% 1361464 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,858,929 A→C T613P (ACC→CCC)  mutS → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,858,9290AC100.0% 164.3 / NA 43T613P (ACC→CCC) mutSmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/21);  total (22/21)

ACCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCACCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATG  >  NC_000913/2858782‑2859076
                                                                                                                                                   |                                                                                                                                                   
aCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATcccc                                                                                                                                                   <  2:130966/150‑1 (MQ=255)
aCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCg                                                                                                                                                  >  1:636183/1‑151 (MQ=255)
     gAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCg                                                                                                                                             <  1:548285/151‑1 (MQ=255)
       aCTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGaa                                                                                                                                           <  1:139290/151‑1 (MQ=255)
       aCTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGaa                                                                                                                                           <  2:255961/151‑1 (MQ=255)
           cacCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATg                                                                                                                                       <  2:172609/151‑1 (MQ=255)
             ccTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATggg                                                                                                                                     >  1:343358/1‑151 (MQ=255)
               tGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCg                                                                                                                                   >  1:437502/1‑151 (MQ=255)
                   cGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTa                                                                                                                                >  1:43765/1‑150 (MQ=255)
                    gACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTaa                                                                                                                               >  2:450774/1‑150 (MQ=255)
                        ttCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGt                                                                                                                           >  1:118900/1‑150 (MQ=255)
                         tCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGt                                                                                                                           <  1:215490/149‑1 (MQ=255)
                           aTTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCt                                                                                                                       >  2:220442/1‑151 (MQ=255)
                             tGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCtat                                                                                                                     >  2:88870/1‑151 (MQ=255)
                                 aaaCCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATgc                                                                                                                 <  2:661086/151‑1 (MQ=255)
                                    ccGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGcc                                                                                                              >  1:553327/1‑151 (MQ=255)
                                     cGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCa                                                                                                             <  2:28928/151‑1 (MQ=255)
                                         cATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGAcc                                                                                                         >  1:33323/1‑151 (MQ=255)
                                              gCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACt                                                                                                    <  1:356939/151‑1 (MQ=255)
                                                aTTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTg                                                                                                   >  2:229732/1‑150 (MQ=255)
                                                   aCCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTg                                                                                               >  1:282494/1‑151 (MQ=255)
                                                       aaGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCt                                                                                           <  2:285424/151‑1 (MQ=255)
                                                         ggTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGa                                                                                         <  2:658806/151‑1 (MQ=255)
                                                           tCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATg                                                                                       >  2:250331/1‑151 (MQ=255)
                                                            cGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATgg                                                                                      >  1:72603/1‑151 (MQ=255)
                                                            cGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATgg                                                                                      >  2:112015/1‑151 (MQ=255)
                                                                aTCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCt                                                                                   >  2:251834/1‑150 (MQ=255)
                                                                       aGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGc                                                                           >  2:333091/1‑151 (MQ=255)
                                                                           gAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGc                                                                        <  2:644962/150‑1 (MQ=255)
                                                                                   aCTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTAcc                                                                <  1:391340/150‑1 (MQ=255)
                                                                                      gAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCgg                                                              <  2:39810/149‑1 (MQ=255)
                                                                                           aGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACaaaa                                                       <  1:489202/151‑1 (MQ=255)
                                                                                           aGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACaaaa                                                       <  1:361226/151‑1 (MQ=255)
                                                                                             ccATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAg                                                     <  2:343369/151‑1 (MQ=255)
                                                                                                tttATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGCCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGACACAACAAgac                                                   >  1:57018/1‑148 (MQ=255)
                                                                                                      gCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTg                                            <  2:540634/151‑1 (MQ=255)
                                                                                                       ccAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTgg                                           >  1:321709/1‑151 (MQ=255)
                                                                                                       ccAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTgg                                           <  2:67901/151‑1 (MQ=255)
                                                                                                                 gAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTatcga                                  <  2:218255/150‑1 (MQ=255)
                                                                                                                 gAATCTGTCGCCGCAGAGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTatcg                                   >  1:384747/1‑149 (MQ=255)
                                                                                                                       gTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGc                              <  1:546525/148‑1 (MQ=255)
                                                                                                                       gTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATc                           >  1:83627/1‑151 (MQ=255)
                                                                                                                          gccgcAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCttt                        <  1:346397/151‑1 (MQ=255)
                                                                                                                                             atcatcCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGc       >  1:623757/1‑149 (MQ=255)
                                                                                                                                              tcatccCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGc       >  1:431463/1‑148 (MQ=255)
                                                                                                                                                atccCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATg  >  1:546843/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCACCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATG  >  NC_000913/2858782‑2859076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A