Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,582	T→C	Y35C (TAC→TGC)	mazG ←	nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,582	0	T	C	100.0%	138.2 / NA	37	Y35C (TAC→TGC)	mazG	nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/18); total (19/18)

ATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGG  >  NC_000913/2910437‑2910717

atCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGTGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGgc                                                                                                                                    <  1:660082/151‑1 (MQ=255)

  cATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGgcg                                                                                                                                   >  2:237813/1‑150 (MQ=255)

      aaaGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAtg                                                                                                                              >  1:367010/1‑151 (MQ=255)

      aaaGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAt                                                                                                                               <  1:243077/150‑1 (MQ=255)

       aaGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAtgg                                                                                                                             <  2:313777/151‑1 (MQ=255)

       aaGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAtgg                                                                                                                             >  1:278240/1‑151 (MQ=255)

        aGTCAACGCGCCCTTCGTCCTGAGCCATTTGCGCGTAAAACACCGCCTGGAATAGCAGATCGCCCAGTTCCCCGTGTAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAtgg                                                                                                                             <  1:136089/150‑1 (MQ=255)

        aGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAtggt                                                                                                                            >  2:652756/1‑151 (MQ=255)

                 gcCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGt                                                                                                                   >  2:301907/1‑151 (MQ=255)

                 gcCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGt                                                                                                                   >  1:300838/1‑151 (MQ=255)

                        ttcCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGctct                                                                                                            >  1:370468/1‑151 (MQ=255)

                        ttcCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGctct                                                                                                            >  1:517287/1‑151 (MQ=255)

                                     tGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGc                                                                                               >  1:20326/1‑151 (MQ=255)

                                      gcgcGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCa                                                                                              <  2:144361/151‑1 (MQ=255)

                                      gcgcGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTAGCCCACGGGCa                                                                                              <  1:411553/151‑1 (MQ=255)

                                       agcgTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGc                                                                                               >  1:227995/2‑149 (MQ=255)

                                          gTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGcc                                                                                           <  1:149459/150‑1 (MQ=255)

                                                      cTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATc                                                                               >  1:53419/1‑150 (MQ=255)

                                                       tGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATcgc                                                                             >  1:125517/1‑151 (MQ=255)

                                                         gAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCgcgc                                                                           >  1:309564/1‑151 (MQ=255)

                                                             aGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGgc                                                                       <  1:117687/151‑1 (MQ=255)

                                                                aGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCt                                                                    >  1:400701/1‑151 (MQ=255)

                                                                      cccAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCataa                                                              <  1:198231/151‑1 (MQ=255)

                                                                                 gcgAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCaa                                                    >  1:367728/1‑150 (MQ=255)

                                                                                     aGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGt                                               >  2:413329/1‑151 (MQ=255)

                                                                                         cGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGAt                                           <  1:533167/151‑1 (MQ=255)

                                                                                            cAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGAttt                                         <  2:220467/150‑1 (MQ=255)

                                                                                                    tCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCAtt                                 <  2:437752/150‑1 (MQ=255)

                                                                                                    tCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCAtt                                 <  2:411460/150‑1 (MQ=255)

                                                                                                         ggCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTg                           >  1:349912/1‑151 (MQ=255)

                                                                                                         ggCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTg                           <  1:215440/151‑1 (MQ=255)

                                                                                                           cGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTc                         <  1:376174/151‑1 (MQ=255)

                                                                                                            gATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTcc                        <  2:50715/151‑1 (MQ=255)

                                                                                                               ggCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTg                      <  2:182707/150‑1 (MQ=255)

                                                                                                                           ttCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGt          >  1:184895/1‑150 (MQ=255)

                                                                                                                           ttCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTc         >  2:261628/1‑151 (MQ=255)

                                                                                                                                  gTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAgg  <  2:495443/151‑1 (MQ=255)

ATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGG  >  NC_000913/2910437‑2910717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A