Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,122,268	T→C	D513G (GAC→GGC)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,122,268	0	T	C	100.0%	172.4 / NA	46	D513G (GAC→GGC)	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/26); total (20/26)

GGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCA  >  NC_000913/3122122‑3122415

gggCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCg                                                                                                                                                  <  2:683669/150‑1 (MQ=255)

          ggCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCcac                                                                                                                                       <  1:429826/151‑1 (MQ=255)

            cTTGCACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGTAGCGTAGCAGCGGTTGGTGACGGCACCCAGGCTGGGTTTGCCCCGGCACGCGGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCCTCAGGCCCGGCATTGCCcaca                                                                                                                                      >  1:46331/1‑150 (MQ=255)

             ttGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATg                                                                                                                                    >  1:367870/1‑151 (MQ=255)

              tGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATg                                                                                                                                    <  1:322775/150‑1 (MQ=255)

                 aCGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCt                                                                                                                                 <  2:660873/150‑1 (MQ=255)

                    ctctGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTAc                                                                                                                             >  1:249304/1‑151 (MQ=255)

                      ctGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTAcc                                                                                                                            >  2:330183/1‑150 (MQ=255)

                            gTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTgc                                                                                                                     >  2:432291/1‑151 (MQ=255)

                             ttggtttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTgc                                                                                                                     >  1:416152/1‑150 (MQ=255)

                              tggtttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCACTGCCCACATGCCTTTACCAATTTgcgc                                                                                                                   >  1:671784/1‑151 (MQ=255)

                               ggtttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCt                                                                                                                  <  2:331415/151‑1 (MQ=255)

                                  ttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCtt                                                                                                                                <  1:317758/134‑1 (MQ=255)

                                  ttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCtt                                                                                                                                >  2:317763/1‑134 (MQ=255)

                                                gcgcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCcaca                                                                                                 <  2:686038/151‑1 (MQ=255)

                                                      ggAGCGTAGCAGCGTTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTTCATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCacagaacag                                                                                           <  1:103506/151‑1 (MQ=255)

                                                         gCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGAcc                                                                                        <  2:32978/151‑1 (MQ=255)

                                                           gTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCgg                                                                                      <  2:371401/151‑1 (MQ=255)

                                                              gcaggGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTGGGTCGCGCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCGTTGCCAATTGGCGCTTTACCGTGCAGCCCACAGAACAGTCCGGaaa                                                                                   <  1:547550/151‑1 (MQ=255)

                                                              gcagcGGTTGGTGACGGAACCCAGGCTGTGGTTGCCCCCGCACGCAGTTGGTCGCCCTTCTGGCTGGACATGTCTGCCATCAGGCCCGGCATAGCCCACAGGCCTTAACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGaa                                                                                    <  1:299384/150‑1 (MQ=255)

                                                                agcGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGc                                                                                 >  1:527141/1‑151 (MQ=255)

                                                                      tGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGtt                                                                            <  2:367883/150‑1 (MQ=255)

                                                                       ggTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGttat                                                                          <  1:562627/151‑1 (MQ=255)

                                                                                  ccAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAg                                                               <  2:409895/151‑1 (MQ=255)

                                                                                      gCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCtt                                                           <  2:122337/151‑1 (MQ=255)

                                                                                        tgtgTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCttt                                                          >  2:293855/1‑150 (MQ=255)

                                                                                        tgtgTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTg                                                         >  2:470845/1‑151 (MQ=255)

                                                                                         gtgtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCttt                                                          <  2:18886/149‑1 (MQ=255)

                                                                                         gtgtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCttt                                                          <  2:258714/149‑1 (MQ=255)

                                                                                          tgtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGAt                                                       >  1:95101/1‑151 (MQ=255)

                                                                                           gtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGAt                                                       <  2:682628/150‑1 (MQ=255)

                                                                                             ttGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCCCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATcc                                                     >  2:151383/1‑150 (MQ=255)

                                                                                                 cccGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGc                                                <  1:25875/151‑1 (MQ=255)

                                                                                                    ggCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTc                                             <  2:395840/151‑1 (MQ=255)

                                                                                                           aGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTc                                       >  1:354095/1‑150 (MQ=255)

                                                                                                                    cccTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGAtttt                             >  2:353075/1‑151 (MQ=255)

                                                                                                                       ttCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCGTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTAc                          >  1:407929/1‑151 (MQ=255)

                                                                                                                        tCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTa                           <  1:586668/149‑1 (MQ=255)

                                                                                                                         cTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTa                           <  1:516080/148‑1 (MQ=255)

                                                                                                                         cTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTa                           <  1:136711/148‑1 (MQ=255)

                                                                                                                           ggCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACgc                        >  2:459322/1‑149 (MQ=255)

                                                                                                                                 aCATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCgg                <  2:324607/151‑1 (MQ=255)

                                                                                                                                  cATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCggg               >  2:61109/1‑151 (MQ=255)

                                                                                                                                         gCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCtt        >  1:645248/1‑151 (MQ=255)

                                                                                                                                               aGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATc   >  2:14659/1‑150 (MQ=255)

                                                                                                                                               aGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCa  <  2:549187/151‑1 (MQ=255)

GGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCA  >  NC_000913/3122122‑3122415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A