Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,439,996	G→A	R2C (CGC→TGC)	rplQ ←	50S ribosomal subunit protein L17

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,439,996	0	G	A	97.7%	166.6 / NA	44	R2C (CGC→TGC)	rplQ	50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (19/24); minor base C (0/1); total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTT  >  NC_000913/3439851‑3440141

ggCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATg                                                                                                                                               <  1:604847/150‑1 (MQ=255)

      aCTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTAt                                                                                                                                        >  1:198254/1‑151 (MQ=255)

                 cAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTa                                                                                                                             <  1:19306/151‑1 (MQ=255)

                  aGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTa                                                                                                                             >  2:607677/1‑150 (MQ=255)

                      ctTTCGCTTTAGGCAGAGTCTTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTaaaa                                                                                                                          <  1:566214/149‑1 (MQ=255)

                        ttCGCTTTAGGCAGAGTCTTCTTGATGATTTCATGACGAACCGGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAAcc                                                                                                                        <  1:27123/149‑1 (MQ=255)

                           gCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAAc                                                                                                                   <  2:214649/151‑1 (MQ=255)

                              ttAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCt                                                                                                                 <  1:147669/150‑1 (MQ=255)

                                aGGCAGAGTCGTCTGGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCtgt                                                                                                               <  1:164829/150‑1 (MQ=255)

                                 ggCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGa                                                                                                             <  2:593369/151‑1 (MQ=255)

                                    agagTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATcc                                                                                                          >  2:75276/1‑151 (MQ=255)

                                     gagCCGTCTTAATGATTTAATGACGAGCGAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCg                                                                                                         <  1:56142/151‑1 (MQ=255)

                                               gatgatTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGt                                                                                                <  2:635597/150‑1 (MQ=255)

                                                 tgatTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTc                                                                                               >  2:447119/1‑149 (MQ=255)

                                                     ttCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGAt                                                                                         >  2:319959/1‑151 (MQ=255)

                                                         tGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCtt                                                                                     >  1:138582/1‑151 (MQ=255)

                                                              aaCCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCg                                                                                 <  1:156619/150‑1 (MQ=255)

                                                                 cAGTGACCCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGTCCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGt                                                                               <  2:89680/149‑1 (MQ=255)

                                                                 cAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTgg                                                                             >  2:573677/1‑151 (MQ=255)

                                                                          tGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTc                                                                    >  1:407072/1‑151 (MQ=255)

                                                                            ccATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCa                                                                  <  2:387519/151‑1 (MQ=255)

                                                                                 ttGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGgcgc                                                             >  1:570750/1‑151 (MQ=255)

                                                                                    cGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATg                                                          <  1:615372/151‑1 (MQ=255)

                                                                                    cGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATg                                                          <  2:477819/151‑1 (MQ=255)

                                                                                     ggAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGc                                                         >  2:45480/1‑151 (MQ=255)

                                                                                           tAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCaga                                                    <  1:534669/150‑1 (MQ=255)

                                                                                            aGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAgaga                                                  <  2:309528/151‑1 (MQ=255)

                                                                                             gCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGAc                                                 >  2:380037/1‑151 (MQ=255)

                                                                                              ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCCCATGACCTTATCCTTCTCAGTAAACCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACa                                                <  1:93941/151‑1 (MQ=255)

                                                                                                  gCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTcc                                            <  1:50535/151‑1 (MQ=255)

                                                                                                    gATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCAc                                          >  1:371385/1‑151 (MQ=255)

                                                                                                     aTGGCTGCTGTTGCGGTTTAGTTGACGACCACTCTTGCTATGGCACATGACCTTATCCTTCTCAGTAAATCCTTAACCTGTGAGCCGGTTACTCGTCAGGGATGCATGCCGGTGGCCAGTTTTCCAGGCGCATTCCCAGAGACATTCCACg                                         <  1:124495/151‑1 (MQ=255)

                                                                                                       ggCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACggg                                       <  1:237724/151‑1 (MQ=255)

                                                                                                              gTTGCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCa                                 <  2:37326/150‑1 (MQ=255)

                                                                                                                 gCGGTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCAc                             <  2:122646/151‑1 (MQ=255)

                                                                                                                   ggTTCAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGt                           >  2:520492/1‑151 (MQ=255)

                                                                                                                       cAGTTGACGACCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCttt                       >  2:437043/1‑151 (MQ=255)

                                                                                                                               gacCACTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtctc                 >  1:561794/1‑149 (MQ=255)

                                                                                                                               gacCACTCGTACGATGGCACATGACCTTATCCTTCTCCGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGACGGTGGCCAGTTTTCCAGGCGCATGCCCACAGACAGTCCACGGGAAGCCCGCACGTCTTTAAtctc                 >  1:22114/1‑149 (MQ=255)

                                                                                                                                   aCTCTTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTa             >  1:675880/1‑149 (MQ=255)

                                                                                                                                      ctTACGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAaga          <  2:574399/149‑1 (MQ=255)

                                                                                                                                         acgacgGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAttt     >  1:610942/1‑151 (MQ=255)

                                                                                                                                         aCGATGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAttt     >  1:315772/1‑151 (MQ=255)

                                                                                                                                            aTGGCACATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtttttt  <  1:226656/151‑1 (MQ=255)

GGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTT  >  NC_000913/3439851‑3440141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A