Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,723,735	T→C	E221E (GAA→GAG)	glyS ←	glycine tRNA synthetase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,723,735	0	T	C	100.0%	183.3 / NA	48	E221E (GAA→GAG)	glyS	glycine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/23); total (25/23)

AGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGTTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGAATGCCCAGAATGGTTGCCGGAAT  >  NC_000913/3723589‑3723881

aGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGctctt                                                                                                                                                >  2:280230/1‑151 (MQ=255)

  gAATTTCTCTTCGAATTTTGCGGTCAGAACGCCCGGCCACTCCCCCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCg                                                                                                                                              <  1:659794/151‑1 (MQ=255)

          ctTCGAATTTTGCGGTCAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCg                                                                                                                                        <  2:117167/149‑1 (MQ=255)

            tCGAATTTTGCGGTCAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCgg                                                                                                                                       <  1:489476/148‑1 (MQ=255)

            tCGAATTTTGCGGTCAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCg                                                                                                                                     <  2:205676/150‑1 (MQ=255)

            tCGAATTTTGCGGTCAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCg                                                                                                                                     <  2:576460/150‑1 (MQ=255)

                        gTCAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCAc                                                                                                                        <  2:184415/151‑1 (MQ=255)

                          cAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGc                                                                                                                      >  2:558085/1‑151 (MQ=255)

                             aaCGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTc                                                                                                                    <  1:316043/150‑1 (MQ=255)

                                gACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGc                                                                                                                >  2:158656/1‑151 (MQ=255)

                                  ccGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAg                                                                                                              <  2:148012/151‑1 (MQ=255)

                                   cGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGa                                                                                                             <  2:98741/151‑1 (MQ=255)

                                       cACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAAttt                                                                                                         >  1:128685/1‑151 (MQ=255)

                                         ccccaccaGCGATGCCACTTCTTCGAGCAGGCGTTCGCTTAATACATCGTTCCCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTc                                                                                                        <  1:146413/148‑1 (MQ=255)

                                            caccaGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCgg                                                                                                     >  1:307830/1‑150 (MQ=255)

                                                aGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACt                                                                                                >  2:434528/1‑151 (MQ=255)

                                                  cGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTg                                                                                               >  2:447604/1‑150 (MQ=255)

                                                       ccACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGc                                                                                         >  2:489520/1‑151 (MQ=255)

                                                        cACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCg                                                                                        >  2:221471/1‑151 (MQ=255)

                                                            tcttcCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGtt                                                                                      <  2:122247/149‑1 (MQ=255)

                                                                     agGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTg                                                                            >  2:458953/1‑150 (MQ=255)

                                                                       gCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAAt                                                                         >  2:252854/1‑151 (MQ=255)

                                                                          ttCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTc                                                                       <  2:632799/150‑1 (MQ=255)

                                                                            cGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCgg                                                                    >  2:631045/1‑151 (MQ=255)

                                                                               ttAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTc                                                                 >  2:348636/1‑151 (MQ=255)

                                                                                 aaGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGc                                                               <  1:403459/151‑1 (MQ=255)

                                                                                     cAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCAt                                                           <  1:443717/151‑1 (MQ=255)

                                                                                      aGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATa                                                          <  1:120782/151‑1 (MQ=255)

                                                                                          ttACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGc                                                      >  1:682450/1‑151 (MQ=255)

                                                                                           tACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGc                                                      <  2:422884/150‑1 (MQ=255)

                                                                                             ccGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCGCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCgg                                                    >  1:410085/1‑150 (MQ=255)

                                                                                                ccAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGc                                                >  2:625264/1‑151 (MQ=255)

                                                                                                  aaTCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCcg                                              >  2:322766/1‑151 (MQ=255)

                                                                                                   aTCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCcgc                                             >  2:16111/1‑151 (MQ=255)

                                                                                                    tCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCcgcg                                            >  1:101901/1‑151 (MQ=255)

                                                                                                         cgcgCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCa                                       <  2:495165/151‑1 (MQ=255)

                                                                                                               gctTCTTCGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGAt                                 <  2:397302/151‑1 (MQ=255)

                                                                                                                 ttcctcGGCATCGGCTTTAATCTTCGCCTTAAGCTCCTCATACTCGTCGATGACTTTCCCACGCTCACGCAGATTTACCGGATACTGATCGGCGTTATCGATGGTGAATTCCGCCTCTCCCATAAACCGACGGCCGCGACTCACGCGATc                                >  1:424881/1‑150 (MQ=255)

                                                                                                                  tcttcGGCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATc                                <  1:508579/149‑1 (MQ=255)

                                                                                                                       ggCATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGa                         <  2:385937/151‑1 (MQ=255)

                                                                                                                         cATCGGCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGAAt                       >  1:82961/1‑151 (MQ=255)

                                                                                                                              gCTTTAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGAATGccc                   >  1:250582/1‑150 (MQ=255)

                                                                                                                                 ttAATCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGAATGCCCAGa                >  2:168019/1‑150 (MQ=255)

                                                                                                                                   aaTCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGAATGCCCAGAATg             <  1:416994/151‑1 (MQ=255)

                                                                                                                                    aTCTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGACTTTCCGGATACTGATCGCCGTTATCGCTGGTGAATTCCGGCTCGCCCATAAAGCGCTGGCCGCGAATCACGCGATCGGACTGAATGCCCAGaa               >  1:684917/1‑148 (MQ=255)

                                                                                                                                      cTTCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGAATGCCCAGAATGGtt          >  2:35549/1‑151 (MQ=255)

                                                                                                                                        tCGCCTTACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGAATGCCCAGAATGGtt          <  1:321910/149‑1 (MQ=255)

                                                                                                                                              tACGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGAATGCCCAGAATGGTTGCCGGAAt  >  2:276216/1‑151 (MQ=255)

                                                                                                                                               aCGCTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGAATGCCCAGAATGGTTGCCGGAAt  <  2:20192/150‑1 (MQ=255)

AGGAATTTCTCTTCGAATTTTGCGGTCAGAACGACCGGCCACTCCACCAGCGAAGCCACTTCTTCCAGCAGGCTTTCGCTTAAGTCAGCGTTACCGCCAATCTTACGCGCTGCTTCTTCGGCATCGGCTTTAATCTTCGCCTTACGTTCTTCGTAATCGGCGATGACTTTCCCACGCTCACGCAGAATTTCCGGATACTGATCGGCGTTATCGATGGTGAATTCCGGCTCGCCCATAAAGCGGTGGCCGCGAATCACGCGATCGGACTGAATGCCCAGAATGGTTGCCGGAAT  >  NC_000913/3723589‑3723881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A