Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,988,580	T→C	K163E (AAA→GAA)	hemX ←	putative uroporphyrinogen III methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,988,580	0	T	C	100.0%	170.4 / NA	44	K163E (AAA→GAA)	hemX	putative uroporphyrinogen III methyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/24); total (20/24)

AAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTGGACTTCATCCAAC  >  NC_000913/3988439‑3988725

aaaGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGc                                                                                                                                          >  2:314547/1‑151 (MQ=255)

  aGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTg                                                                                                                                        <  2:5528/151‑1 (MQ=255)

     tGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGc                                                                                                                                       >  2:196128/1‑149 (MQ=255)

          aaGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTc                                                                                                                                <  1:390785/151‑1 (MQ=255)

            gCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGt                                                                                                                              >  1:542819/1‑151 (MQ=255)

            gCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGt                                                                                                                              <  2:464754/151‑1 (MQ=255)

                    atgatgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCt                                                                                                                      <  1:518488/151‑1 (MQ=255)

                    atgatgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCt                                                                                                                      <  1:48992/151‑1 (MQ=255)

                      gatgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTg                                                                                                                     <  1:382986/150‑1 (MQ=255)

                      gatgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGa                                                                                                                    <  2:422121/151‑1 (MQ=255)

                            gTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTc                                                                                                              >  2:637683/1‑151 (MQ=255)

                            gTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTc                                                                                                              <  2:363717/151‑1 (MQ=255)

                              cATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCca                                                                                                            <  2:476354/151‑1 (MQ=255)

                                tAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCcac                                                                                                           >  1:278817/1‑150 (MQ=255)

                                   tCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCACCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCt                                                                                                       >  1:368218/1‑151 (MQ=255)

                                      aCCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTtcc                                                                                                    <  2:119531/151‑1 (MQ=255)

                                              tACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCCCCGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCgg                                                                                              <  1:189266/149‑1 (MQ=255)

                                                   cAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGttt                                                                                       >  2:482340/1‑151 (MQ=255)

                                                     gAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTc                                                                                      >  1:584538/1‑150 (MQ=255)

                                                      aaaGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCAc                                                                                    >  2:338201/1‑151 (MQ=255)

                                                       aaGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCAcc                                                                                   <  1:250423/151‑1 (MQ=255)

                                                        aGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCAcc                                                                                   >  2:160909/1‑150 (MQ=255)

                                                               cGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATc                                                                           <  2:659588/151‑1 (MQ=255)

                                                                       tcGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTg                                                                     >  1:29731/1‑149 (MQ=255)

                                                                              ttGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCcagc                                                              >  2:345641/1‑149 (MQ=255)

                                                                                       gaACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGtttt                                                   >  1:321264/1‑151 (MQ=255)

                                                                                            gTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGACAGCAGCCAGGTTTTAGCAt                                              <  2:122525/151‑1 (MQ=255)

                                                                                              aaTCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTTCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATc                                             <  1:498212/150‑1 (MQ=255)

                                                                                                 cAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCt                                          <  1:637648/150‑1 (MQ=255)

                                                                                                 cAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCt                                          <  1:94258/150‑1 (MQ=255)

                                                                                                  aGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGc                                        <  2:24812/151‑1 (MQ=255)

                                                                                                  aGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGc                                        <  2:422687/151‑1 (MQ=255)

                                                                                                            cattcatATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAAtgg                               <  1:502858/150‑1 (MQ=255)

                                                                                                              ttcatATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAAtggtgg                            >  1:562400/1‑151 (MQ=255)

                                                                                                                   atCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGTCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGAcc                       <  2:607085/151‑1 (MQ=255)

                                                                                                                   atCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGAcc                       >  1:241410/1‑151 (MQ=255)

                                                                                                                    tCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGAcc                       <  1:373403/150‑1 (MQ=255)

                                                                                                                      cGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCttt                    >  1:599886/1‑151 (MQ=255)

                                                                                                                         cAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTttgt                 >  1:654136/1‑151 (MQ=255)

                                                                                                                          aGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTttgtt                >  1:453522/1‑151 (MQ=255)

                                                                                                                             cTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTgg              >  2:456814/1‑150 (MQ=255)

                                                                                                                               ggCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTGGa             <  1:662755/149‑1 (MQ=255)

                                                                                                                               ggCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTGGACt           <  1:656411/151‑1 (MQ=255)

                                                                                                                                        aCTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTGGACTTCATCCAAc  >  1:310230/1‑151 (MQ=255)

AAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTGGACTTCATCCAAC  >  NC_000913/3988439‑3988725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A