Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I1 R1
|
144 |
141.8 |
4677362 |
93.6% |
4378010 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,097,480 |
A→G |
intergenic (+651/+85) |
ycdU → / ← serX |
putative inner membrane protein/tRNA‑Ser |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,097,480 | 0 | A | G | 86.4%
| 93.6
/ 6.0
| 46 | intergenic (+651/+85) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base G (15/23); total (20/26) |
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 |
GTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCT > NC_000913/1097331‑1097627
|
gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGaa < 2:1215460/151‑1 (MQ=11)
atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGG‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAACCCCg < 1:1645472/151‑1 (MQ=9)
atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAACCCCg < 2:845484/151‑1 (MQ=14)
tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GA‑GGGGGGGATTGACGCCCCGCGCTCTCCCCTCGGGCAGCCCCCCCCCCGCGTTCCCGGTCCTTTCAACCGGCTGCGGCCCGGT‑GGCCAAccccc > 1:1418811/1‑148 (MQ=2)
ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTTGACTCGCCTCGCTCGCCCCGCGGGCAGCCCCCCCACTGCGTTTCCGGTCTGTCCAACTGGGTGTCGCCCGTT‑GTCGGACCCCgg > 2:158656/1‑150 (MQ=11)
taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTTGACTCGCCTTCCCCCCCCCGCGGGCAGCCCCCCCACCGCGTTCAAGGGCTGGTCAACCCGCCGTTGCCCGTTGGTCGAACCCCg > 2:467607/1‑149 (MQ=2)
cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCg < 2:1634115/150‑1 (MQ=14)
aCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGCAGGGGGGagtg > 1:1377048/1‑145 (MQ=0)
ttCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAgcccc > 2:518346/1‑150 (MQ=9)
tCACGGTCTGTCCCTCTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAAc < 1:979126/145‑1 (MQ=16)
cACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAccccc > 1:197239/1‑148 (MQ=25)
cACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGAt < 2:80991/151‑1 (MQ=38)
cACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGAt < 1:204791/151‑1 (MQ=38)
cACGGTCTGCCCAACTGTCTGTTGTCGCTT‑ATCCACCTTCCCTCCGCGCCTCTCTTCCGCTCTGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAAcc < 4:86920/145‑1 (MQ=2)
cGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAc < 2:39555/151‑1 (MQ=255)
ggTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGTTTGAAacccc > 1:1528029/1‑145 (MQ=255)
ggTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGCGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATa < 1:530546/149‑1 (MQ=255)
ggTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCGCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTCGGGGGGGGGtttaccctgattcctc > 4:304603/1‑136 (MQ=1)
tGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAACCCCCCCTACGtt > 2:1194954/1‑149 (MQ=255)
gTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAcccccctttcccgccc > 2:351983/1‑140 (MQ=1)
gTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAAACCCCGCTaag > 2:1192603/1‑144 (MQ=11)
gTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGtt < 2:1377198/148‑1 (MQ=255)
gTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTg < 3:277155/149‑1 (MQ=255)
gTCCAACTGGCTGGCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTTGCAACCCCCATaacgt > 4:208496/1‑144 (MQ=11)
tCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGATGGGGGGGTTCGAAcccccccca > 1:1596069/1‑139 (MQ=255)
cAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGta < 2:1562319/151‑1 (MQ=255)
cAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGGTCGAAACCCCCCTACGTTGcc > 2:827537/1‑148 (MQ=11)
aaCTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGtat < 2:1475750/151‑1 (MQ=255)
tGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAAACCCCCCTACCGTGCCGtata > 2:101023/1‑149 (MQ=11)
tGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGAGGGGGGGGGTCGAACCCCCGATAAGCTGCCgcaaa > 1:186518/1‑145 (MQ=14)
ggCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATaca < 2:1165240/150‑1 (MQ=255)
gCTGTCGCGGGTT‑TTCGAGCCCCGGTCGGGGCTTTTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTGCTTTCGTACGAGCTCTTCGTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATgca < 2:1006921/149‑1 (MQ=17)
tGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAACCCCCGCTACgctcccctctaccc > 1:1195495/1‑135 (MQ=11)
gTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATacacac < 2:1596533/149‑1 (MQ=255)
gTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACtt < 1:1533202/151‑1 (MQ=255)
gTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACt < 2:483276/150‑1 (MQ=255)
gTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACt < 2:788829/150‑1 (MQ=255)
gTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACt < 1:1384254/150‑1 (MQ=255)
ccAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCa < 2:590190/151‑1 (MQ=255)
cAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAg < 2:1634368/151‑1 (MQ=255)
cAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAg < 4:189338/151‑1 (MQ=255)
aGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTAACGGGGGGGGTCGGAACCCCCCCACGTTTTCGGATacacaaacttccgg > 2:814292/1‑142 (MQ=11)
aGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGATCGAAACCCCCATTACTTGCCGcattaacccctt > 2:1006292/1‑137 (MQ=11)
gTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCa < 3:186142/148‑1 (MQ=255)
tt‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGATCCCCCGATACGTTGCCGTATACACTCTTTCCAg < 1:1612569/148‑1 (MQ=255)
t‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACGTTGCCGTATACACACTTTTCCGGCg > 4:351304/1‑150 (MQ=255)
cGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCt < 2:1486082/151‑1 (MQ=255)
|
GTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCT > NC_000913/1097331‑1097627
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
