Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I1 R1 144 141.8 4677362 93.6% 4378010 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,154,782 A→G E224G (GAA→GGA)  yceG → septation protein, ampicillin sensitivity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,154,7820AG99.3% 550.6 / NA 148E224G (GAA→GGA) yceGseptation protein, ampicillin sensitivity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (72/75);  minor base T (1/0);  total (73/75)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 148 total aligned reads displayed.

TCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTAT  >  NC_000913/1154635‑1154924
                                                                                                                                                     |                                                                                                                                              
  gcgTTCCACAAGCGAGCGAACAAGAAATGGGTGAAAGGGGTCAATACCGCGTGGGAAGGGCGGGCGGAGGTTCTGTCTTATAAAGAAAAAAACCAGTT‑GGTGATGATGGCATAAATTAGCA‑AAAAAGAAACCGCCGTTGCCAGTGGAc                                                                                                                                              <  1:1170518/148‑1 (MQ=255)
     ttACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGAt                                                                                                                                         >  2:930321/1‑150 (MQ=255)
     ttACTCAAGCGAGCGCAAAAGAAAAGGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATAAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGa                                                                                                                                          <  3:449905/149‑1 (MQ=255)
        cTCAGGCGAGGGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGGGGACGGTCTGCTTTATAAAGATAAAAACCAGTG‑GGTGACGATGGCATAAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATaa                                                                                                                                       <  1:402050/149‑1 (MQ=255)
         tCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGt                                                                                                                                    >  4:268513/1‑151 (MQ=255)
          cAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGt                                                                                                                                    >  1:1267934/1‑151 (MQ=255)
           aaGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGAAAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAgg                                                                                                                                     <  2:1007204/148‑1 (MQ=255)
           aaGCGAGCGCACAAAAAAAGGGGAAAAGGGGTAAATAGCGCCTGGGAAGGGCGGGCGGACGGTCTGCCTTATAAAGATAAAAACCGGTG‑GGTGACGATGGAATCAATTATCG‑AAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGtt                                                                                                                                   <  2:1702068/150‑1 (MQ=255)
           aaGCGAGCGCAAAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAgg                                                                                                                                     <  2:1541797/148‑1 (MQ=255)
            agcgagcgCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGc                                                                                                                                 >  3:59686/1‑151 (MQ=255)
             gcgagcgCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGGGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGGGGACGCGATAAGGTTGcc                                                                                                                                >  2:1280591/1‑151 (MQ=255)
               gagcgCACAAGAAAATGGTGAAAGCGGTGGATAGCGCCTGGGAAGGGAGAGCGGACAGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGTACGCGATAAGGTTGCCTc                                                                                                                              >  1:1479926/1‑151 (MQ=255)
                 gcgcACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTc                                                                                                                              >  1:1625016/1‑149 (MQ=255)
                     ccaAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAtt                                                                                                                        >  1:1403085/2‑151 (MQ=255)
                      caAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAttt                                                                                                                       >  1:639875/1‑151 (MQ=255)
                      caAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAttt                                                                                                                       >  2:817700/1‑151 (MQ=255)
                           aaaTGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCa                                                                                                                   >  3:513173/1‑150 (MQ=255)
                            aaTGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAACCCAGTG‑GGTGACGATGGCATCAATTATCA‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAAc                                                                                                                 <  2:767131/151‑1 (MQ=255)
                              tGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCg                                                                                                               >  4:554230/1‑151 (MQ=255)
                                gTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGtt                                                                                                             >  2:167580/1‑151 (MQ=255)
                                   aaaGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTAAAAAGAAAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTa                                                                                                           <  4:459571/150‑1 (MQ=255)
                                        gggcGAAAGCCCCTGGGAAGGCCGGGGGGACGGTCTGCCTTATAAAGAAAAAAACCATTT‑GGTGACGGTGGCACCAATTATCA‑AAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCa                                                                                                       <  2:1403239/146‑1 (MQ=255)
                                            gATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTgg                                                                                                   <  2:421690/149‑1 (MQ=255)
                                             atggcgcCGGGGAAGGGCGTGCGAAGGGTCTGCCTTAAAAAGAAAAAACCCAGTT‑GGGGACGATGGTTTAAATTATGG‑AAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCAt                                                                                                      <  1:594578/142‑1 (MQ=255)
                                                 cgcCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATgcgc                                                                                            >  2:1228012/1‑151 (MQ=255)
                                                  gcCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGcc                                                                                           >  2:1229170/1‑151 (MQ=255)
                                                   ccTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCt                                                                                          >  1:1147858/1‑151 (MQ=255)
                                                    cTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTg                                                                                         >  1:1656674/1‑151 (MQ=255)
                                                     tGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGc                                                                                        >  2:1604297/1‑151 (MQ=255)
                                                      gggAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTG‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTg                                                                                         <  2:1311058/149‑1 (MQ=255)
                                                       ggaggggttggCGGACGTGCAGCTTTATAAAGATAAAAAGCGGTT‑TGTGGCGATGGCCTTAATCTCCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAg                                                                                      <  1:1091640/141‑1 (MQ=255)
                                                       gaaaGGGCGTGCGGACGTTCTGCCTTATAAAGAAAAAACCCAGTT‑GGTGACGATGGCATCAATTATCA‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAg                                                                                      <  2:245253/149‑1 (MQ=255)
                                                           gggcgggcGGACGGTCTGCCTTAAAAAGAAAAAACCCAGTT‑GGTGACGATGGAATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccg                                                                                  <  1:104133/151‑1 (MQ=255)
                                                           gggCGTGCGGACGGTCTGCCTTAAAAAGATAAAACCCAGTT‑GGTGACGATGGCGTCAATTATCA‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccg                                                                                  <  2:1436946/151‑1 (MQ=255)
                                                             gCGTGCGGACGGTCTGCCTTAAAAAGATAAAAACCATTT‑GGTGACGAGGGCATAAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccgac                                                                                <  2:316694/151‑1 (MQ=255)
                                                                tGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccg                                                                             >  1:1170716/1‑151 (MQ=255)
                                                                tGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccg                                                                             >  1:391279/1‑151 (MQ=255)
                                                                tGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccg                                                                             >  2:346968/1‑151 (MQ=255)
                                                                tGCGGACGGTCTGCCTTAAAAAGATAAAAACCAGTT‑GTTGACGAAGGCATCATTTATCG‑AAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccgacc                                                                               <  4:539600/149‑1 (MQ=255)
                                                                   ggACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACcgacccgacc                                                                          >  3:226701/1‑151 (MQ=255)
                                                                   ggACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACcgacccgacc                                                                          >  1:1660712/1‑151 (MQ=255)
                                                                     aCGGTCTGCCTTATAAAGATAAAAACCATGG‑GGTGACAATCGATTTAATTTTCA‑AAAAAAAATCCGCCGTTGACAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACcgacccgacc                                                                          <  1:462925/149‑1 (MQ=255)
                                                                       ggTCTGCCTTATAAAGATAAAAACCAGTG‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGa                                                                      <  2:569059/151‑1 (MQ=255)
                                                                            gCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTAc                                                                 >  2:1724154/1‑151 (MQ=255)
                                                                             ccTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACg                                                                >  1:808228/1‑151 (MQ=255)
                                                                             ccTTAAAAAAAAAAAATCCAGTG‑GGGGACGATGGCATCAATTACGG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTAc                                                                 <  1:175673/150‑1 (MQ=255)
                                                                              cTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACgg                                                               >  2:1006699/1‑151 (MQ=255)
                                                                               ttATAAAGATAAAAACCAGTT‑GGTGAGGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTAc                                                                 <  3:130434/148‑1 (MQ=255)
                                                                               ttATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACggg                                                              >  2:935377/1‑151 (MQ=255)
                                                                               ttATAAAGATAAAAACCAGTG‑GGTGACGTTGGCATCATTTATCG‑AAAAATAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACgg                                                               <  1:1677166/150‑1 (MQ=255)
                                                                                 ataAAGATAAAACCAAGTA‑GGTGAGAATGGCATCAATTACCA‑AAAAACACACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTACTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGGCCCGACCGTGATTTACGGGAt                                                            <  1:799821/151‑1 (MQ=255)
                                                                                   aaaaATAAAAACCAGTT‑GTTGACAATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATg                                                           <  1:514449/150‑1 (MQ=255)
                                                                                   aaaGATAAAACCCAGTT‑GGTGAGGATGGCATCAATTATCG‑AAAAAAAATCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATg                                                           <  2:12831/150‑1 (MQ=255)
                                                                                   aaaGATAAAACCCAGTT‑GGTGACGAGGGCGTAAATTATCA‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGa                                                             <  4:220751/148‑1 (MQ=255)
                                                                                   aaaGAAAAAAACCAGTT‑GGTGACGATGGCATAAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATg                                                           <  1:44996/150‑1 (MQ=255)
                                                                                    aaGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                         >  1:421567/1‑151 (MQ=255)
                                                                                     aGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                         >  4:229991/1‑150 (MQ=255)
                                                                                        aaaaaaCCAATTGGGGGAGGAGGGCATCAATTATGA‑AAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGgaga                                                      <  1:1462059/150‑1 (MQ=255)
                                                                                         aaaaaCCAGTG‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGc                                                    <  4:421473/151‑1 (MQ=255)
                                                                                              ccAGTT‑GGTGACGATGCCATAAATTATCA‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTtat                                               <  2:1350607/151‑1 (MQ=255)
                                                                                              ccAGTT‑GGTGACGAGGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTta                                                <  3:595106/150‑1 (MQ=255)
                                                                                                       tGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCACCCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCa                                        >  1:340460/1‑150 (MQ=255)
                                                                                                        gACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaa                                       >  2:652684/1‑150 (MQ=255)
                                                                                                        gACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaa                                       >  1:444195/1‑150 (MQ=255)
                                                                                                        gACGATGGCATCAATTATCA‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaaa                                      <  2:274998/151‑1 (MQ=255)
                                                                                                        gACGATGGCATCAATTACCG‑AAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCa                                        <  1:1643441/149‑1 (MQ=255)
                                                                                                          cGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACt                                    >  1:1279427/1‑151 (MQ=255)
                                                                                                           gagggCAACAATTAACA‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAAc                                     <  1:1441068/146‑1 (MQ=255)
                                                                                                           gATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACtt                                   >  2:602903/1‑151 (MQ=255)
                                                                                                            aTGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACt                                    <  3:483727/149‑1 (MQ=255)
                                                                                                              ggCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACttt                                  <  2:927111/149‑1 (MQ=255)
                                                                                                              ggCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTtct                                <  2:261712/151‑1 (MQ=255)
                                                                                                                cATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCg                              >  1:991786/1‑151 (MQ=255)
                                                                                                                cATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCg                              >  2:330406/1‑151 (MQ=255)
                                                                                                                 aTCATTTATTG‑ATAAAGAAACCGCCGTTGCCAGTGGGCGCGCAATGGTTTCCTCAGCATTAATCAACCGTTTACGCATTGGTAGGCGCGTGGAGAACGACCCGACCGTGATTTACGGGAAGGGAGAGCGTTATAATGGCCAACTTtc                                 >  1:1005727/1‑147 (MQ=255)
                                                                                                                 aTCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCg                              >  2:997044/1‑150 (MQ=255)
                                                                                                                 aTCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             >  2:1079850/1‑151 (MQ=255)
                                                                                                                 aTCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             >  1:324855/1‑151 (MQ=255)
                                                                                                                 aTCAATTATCG‑AAAAAAAACCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             <  2:828626/151‑1 (MQ=255)
                                                                                                                  tCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             >  1:685411/1‑150 (MQ=255)
                                                                                                                  tCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             <  1:1336250/150‑1 (MQ=255)
                                                                                                                      ttATCG‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGc                           >  1:1527076/1‑148 (MQ=255)
                                                                                                                      ttACCG‑AAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAg                         <  4:282160/150‑1 (MQ=255)
                                                                                                                        aTCG‑AAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGAc                       <  2:655803/150‑1 (MQ=255)
                                                                                                                           g‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTgg                   >  1:681245/1‑151 (MQ=255)
                                                                                                                           g‑AAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTgg                   >  3:444215/1‑151 (MQ=255)
                                                                                                                             aaaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGAc                       <  2:776018/146‑1 (MQ=255)
                                                                                                                             aaaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTg                    <  1:698682/149‑1 (MQ=255)
                                                                                                                              aaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGaa                 <  3:422544/151‑1 (MQ=255)
                                                                                                                              aaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGa                  >  2:1375734/1‑150 (MQ=255)
                                                                                                                                   aaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc            >  1:1283403/1‑151 (MQ=255)
                                                                                                                                   aaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc            >  2:434866/1‑151 (MQ=255)
                                                                                                                                   aaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc            <  2:114160/151‑1 (MQ=255)
                                                                                                                                        gccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGAc         >  1:1566902/1‑149 (MQ=255)
                                                                                                                                         ccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGc      >  2:1525477/1‑151 (MQ=255)
                                                                                                                                         ccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGc      >  2:1434083/1‑151 (MQ=255)
                                                                                                                                         ccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGc      <  1:1059886/151‑1 (MQ=255)
                                                                                                                                          cgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCg     >  1:786045/1‑151 (MQ=255)
                                                                                                                                          cgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCg     >  2:112740/1‑151 (MQ=255)
                                                                                                                                            ttGCCAGTGGACGCGGTAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGt    >  2:1334472/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
TCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTT‑GGTGACGATGGCATCAATTATCG‑AAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTAT  >  NC_000913/1154635‑1154924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A