Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I3 R1 143 109.6 3434427 98.5% 3382910 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,109,302 A→G intergenic (‑361/‑33) opgC ← / → opgG OPG biosynthetic transmembrane succinyltransferase/OPG biosynthetic periplasmic beta‑1,6 branching glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,109,3020AG98.3% 425.9 / ‑1.3 117intergenic (‑361/‑33)opgC/opgGOPG biosynthetic transmembrane succinyltransferase/OPG biosynthetic periplasmic beta‑1,6 branching glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (53/62);  total (55/62)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 118 total aligned reads displayed.

CTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCT  >  NC_000913/1109156‑1109452
                                                                                                                                                  |                                                                                                                                                      
cTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGt                                                                                                                                                     >  2:1301375/1‑150 (MQ=255)
  ttAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTg                                                                                                                                                    <  1:1015317/149‑1 (MQ=255)
  ttAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGc                                                                                                                                                   <  2:723535/150‑1 (MQ=255)
  ttAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGc                                                                                                                                                   <  2:1256144/150‑1 (MQ=255)
   tAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCtt                                                                                                                                                 >  1:1060588/1‑151 (MQ=255)
    aGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCtt                                                                                                                                                 <  1:21672/150‑1 (MQ=255)
    aGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTa                                                                                                                                                <  2:1586057/151‑1 (MQ=255)
      gagaAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACt                                                                                                                                              <  2:43850/151‑1 (MQ=255)
      gagaAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACt                                                                                                                                              <  2:1099374/151‑1 (MQ=255)
             aCGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATg                                                                                                                                       >  1:857109/1‑151 (MQ=255)
              cGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATg                                                                                                                                       >  2:1167665/1‑150 (MQ=255)
              cGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATg                                                                                                                                       >  1:398144/1‑150 (MQ=255)
              cGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGa                                                                                                                                      <  2:1215661/151‑1 (MQ=255)
               ggAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGa                                                                                                                                      >  2:526702/1‑150 (MQ=255)
                  aaGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACa                                                                                                                                  <  2:1537242/151‑1 (MQ=255)
                   aGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACAt                                                                                                                                 <  1:100823/151‑1 (MQ=255)
                   aGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACAt                                                                                                                                 <  1:1292711/151‑1 (MQ=255)
                    gTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACAt                                                                                                                                 >  2:1097374/1‑150 (MQ=255)
                      gAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATaaa                                                                                                                              <  2:629284/151‑1 (MQ=255)
                         aaCGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTa                                                                                                                           <  2:1655397/151‑1 (MQ=255)
                           cGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACa                                                                                                                         <  1:1030687/151‑1 (MQ=255)
                             gTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACaaa                                                                                                                       <  1:266846/151‑1 (MQ=255)
                                gCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatga                                                                                                                    <  2:765379/151‑1 (MQ=255)
                                gCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatg                                                                                                                     <  1:738705/150‑1 (MQ=255)
                                 cAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGTTTCTGTTGTCGGAATGGCTGGTTATCCATAAAAATAGATCGGACCGATTTAAGCACAAAAAGGGGGGAGTGCTTACTAATTATGAAACATAACCTACAAatgat                                                                                                                   >  2:1414469/1‑151 (MQ=255)
                                 cAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatgat                                                                                                                   <  1:951738/151‑1 (MQ=255)
                                 cAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatgat                                                                                                                   >  2:517446/1‑151 (MQ=255)
                                 cAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatgat                                                                                                                   <  1:1595658/151‑1 (MQ=255)
                                  aaTCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatgatg                                                                                                                  <  2:764458/151‑1 (MQ=255)
                                  aaTCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatgatg                                                                                                                  <  2:865135/151‑1 (MQ=255)
                                  aaTCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatgatg                                                                                                                  <  2:1624424/151‑1 (MQ=255)
                                   aTCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatgatg                                                                                                                  >  2:1147612/1‑150 (MQ=255)
                                   aTCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatgatg                                                                                                                  >  1:1032637/1‑150 (MQ=255)
                                   aTCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAAtgatga                                                                                                                 <  2:198714/151‑1 (MQ=255)
                                    tCAGGTGCTTAATCCATGGGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAatgatg                                                                                                                  <  2:884364/149‑1 (MQ=255)
                                    tCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAAtgatga                                                                                                                 >  2:1191652/1‑150 (MQ=255)
                                    tCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAAtgatga                                                                                                                 >  2:74448/1‑150 (MQ=255)
                                     cAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGaa                                                                                                                >  2:556475/1‑150 (MQ=255)
                                     cAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGATGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGaa                                                                                                                <  1:1290209/150‑1 (MQ=255)
                                      aGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGaaa                                                                                                               <  1:597789/150‑1 (MQ=255)
                                        gTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATg                                                                                                            >  2:897889/1‑151 (MQ=255)
                                          gCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCg                                                                                                          >  2:1348478/1‑151 (MQ=255)
                                            ttAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCgtt                                                                                                        >  1:183751/1‑151 (MQ=255)
                                             tAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCgt                                                                                                         <  1:1372402/149‑1 (MQ=255)
                                                 ccATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCgttggttg                                                                                                   <  2:1492194/151‑1 (MQ=255)
                                                 ccATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCgttggtt                                                                                                    <  2:1620848/150‑1 (MQ=255)
                                                   aTGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGa                                                                                                  <  1:381865/150‑1 (MQ=255)
                                                   aTGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAg                                                                                                 <  1:1327940/151‑1 (MQ=255)
                                                          aGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGtgctgc                                                                                           >  1:306951/1‑150 (MQ=255)
                                                           gCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGtgctgc                                                                                           >  2:1538504/1‑149 (MQ=255)
                                                           gCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAg                                                                                         <  2:1570321/151‑1 (MQ=255)
                                                           gCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAg                                                                                         <  1:229612/151‑1 (MQ=255)
                                                            cGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAg                                                                                         >  1:1292003/1‑150 (MQ=255)
                                                               gCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAAt                                                                                     <  1:473926/151‑1 (MQ=255)
                                                                       aTACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAAccc                                                                             >  1:1104952/1‑151 (MQ=255)
                                                                       aTACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAAAATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAAccc                                                                             >  1:1229753/1‑151 (MQ=255)
                                                                         aCCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTg                                                                           <  2:354066/151‑1 (MQ=255)
                                                                            gggATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGtat                                                                        <  2:421254/151‑1 (MQ=255)
                                                                                        tCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGc                                                            >  1:63238/1‑151 (MQ=255)
                                                                                         cGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGc                                                            <  2:1325746/150‑1 (MQ=255)
                                                                                          ggAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTg                                                          >  1:59096/1‑151 (MQ=255)
                                                                                           gAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTgg                                                         <  1:47810/151‑1 (MQ=255)
                                                                                            aaTGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTggg                                                        >  2:644636/1‑151 (MQ=255)
                                                                                             aTGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGc                                                       >  2:348105/1‑151 (MQ=255)
                                                                                               ggCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCtt                                                     <  2:924777/151‑1 (MQ=255)
                                                                                               ggCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCt                                                      <  1:655675/150‑1 (MQ=255)
                                                                                                   ggTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTc                                                   <  2:196700/149‑1 (MQ=255)
                                                                                                     ttATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGt                                                <  2:369034/150‑1 (MQ=255)
                                                                                                     ttATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGt                                                >  1:1396368/1‑150 (MQ=255)
                                                                                                      tATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTAt                                              >  2:250878/1‑151 (MQ=255)
                                                                                                        accATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATtg                                            >  2:1672321/2‑151 (MQ=255)
                                                                                                         ccATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATtg                                            >  2:551568/1‑150 (MQ=255)
                                                                                                           aTTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATtgatg                                         <  2:26010/151‑1 (MQ=255)
                                                                                                           aTTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATtgatg                                         >  2:900799/1‑151 (MQ=255)
                                                                                                              aaaaTAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTgatgat                                       <  2:398172/150‑1 (MQ=255)
                                                                                                              aaaaTAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGatgatg                                      >  2:1234672/1‑151 (MQ=255)
                                                                                                               aaaTAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGt                                     >  1:1085555/1‑151 (MQ=255)
                                                                                                               aaaTAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGt                                     >  2:741142/1‑151 (MQ=255)
                                                                                                                aaTAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTc                                    >  2:1107954/1‑151 (MQ=255)
                                                                                                                  tAGATCGGATCGATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGc                                  >  2:787391/1‑151 (MQ=255)
                                                                                                                    gatcggatcgATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCa                                 >  1:1275693/1‑150 (MQ=255)
                                                                                                                            cgATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCt                        >  2:1242452/1‑151 (MQ=255)
                                                                                                                             gATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTg                                                          <  1:472545/116‑1 (MQ=255)
                                                                                                                             gATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTg                                                          >  2:472588/1‑116 (MQ=255)
                                                                                                                             gATATAAGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTc                       <  2:1060670/151‑1 (MQ=255)
                                                                                                                             gATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTc                       >  2:135057/1‑151 (MQ=255)
                                                                                                                                  aaGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATc                   <  1:350615/150‑1 (MQ=255)
                                                                                                                                   aGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCtt                 <  1:877851/151‑1 (MQ=255)
                                                                                                                                   aGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCtt                 <  1:1519524/151‑1 (MQ=255)
                                                                                                                                   aGCACACAAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCtt                 >  1:773260/1‑151 (MQ=255)
                                                                                                                                     cacacaAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTa               <  1:41900/151‑1 (MQ=255)
                                                                                                                                      acacaAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCttt                >  1:1604734/1‑149 (MQ=255)
                                                                                                                                       cacaAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGc             >  2:620136/1‑151 (MQ=255)
                                                                                                                                        acaAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGcc            >  1:33331/1‑151 (MQ=255)
                                                                                                                                        acaAAGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGcc            >  1:566715/1‑151 (MQ=255)
                                                                                                                                          aaaGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCgg          >  1:1667573/1‑151 (MQ=255)
                                                                                                                                          aaaGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCgg          <  1:915283/151‑1 (MQ=255)
                                                                                                                                           aaGGGGGGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCggg         >  2:1362980/1‑151 (MQ=255)
                                                                                                                                              ggggGAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGaaa      <  1:1496448/151‑1 (MQ=255)
                                                                                                                                                  aaGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCt  >  1:1711460/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
CTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCT  >  NC_000913/1109156‑1109452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A