Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I3 R1	143	109.6	3434427	98.5%	3382910	150.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,629,237	T→C	intergenic (‑299/‑53)	yfgF ← / → yfgG	cyclic‑di‑GMP phosphodiesterase, anaerobic/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,629,237	0	T	C	99.1%	398.3 / ‑4.1	107	intergenic (‑299/‑53)	yfgF/yfgG	cyclic‑di‑GMP phosphodiesterase, anaerobic/uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (50/56); total (51/56)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

Only 100 of 109 total aligned reads displayed.

CCTGATTAATGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGTGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGC  >  NC_000913/2629090‑2629382

ccTGATTAATGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGcc                                                                                                                                                >  1:324481/1‑151 (MQ=255)

 cTGATTAATGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCt                                                                                                                                               >  1:1495596/1‑151 (MQ=255)

  tGATTAATGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCtt                                                                                                                                              >  1:211657/1‑151 (MQ=255)

  tGATTAATGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCtt                                                                                                                                              >  2:213193/1‑151 (MQ=255)

   gATTAATGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCtt                                                                                                                                              <  2:1405972/150‑1 (MQ=255)

    aTTAATGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTaa                                                                                                                                            <  1:836213/151‑1 (MQ=255)

      tAATGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAAt                                                                                                                                           <  2:614601/150‑1 (MQ=255)

       aaTGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATg                                                                                                                                          <  1:144086/150‑1 (MQ=255)

         tGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTa                                                                                                                                        >  1:368050/1‑150 (MQ=255)

          gAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTaa                                                                                                                                       <  2:581679/150‑1 (MQ=255)

          gAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTa                                                                                                                                        >  2:752149/1‑149 (MQ=255)

          gAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAg                                                                                                                                      <  2:1583330/151‑1 (MQ=255)

              tattaCGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTAcc                                                                                                                                  >  2:76743/1‑151 (MQ=255)

              tattaCGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTAc                                                                                                                                   <  2:863522/150‑1 (MQ=255)

               attaCGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTAcc                                                                                                                                  <  1:343668/150‑1 (MQ=255)

               attaCGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCg                                                                                                                                 >  1:1619679/1‑151 (MQ=255)

                 taCGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTc                                                                                                                               >  1:231439/1‑151 (MQ=255)

                   cGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTcc                                                                                                                              >  1:485239/1‑150 (MQ=255)

                   cGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCca                                                                                                                             <  1:1006437/151‑1 (MQ=255)

                      ttATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCcaca                                                                                                                           <  2:1282583/150‑1 (MQ=255)

                       tATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAg                                                                                                                          <  1:1343800/150‑1 (MQ=255)

                       tATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAg                                                                                                                          <  2:1125323/150‑1 (MQ=255)

                           aTGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTgg                                                                                                                     >  1:1443235/1‑151 (MQ=255)

                           aTGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTgg                                                                                                                     >  1:1321148/1‑151 (MQ=255)

                             gTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGa                                                                                                                   <  1:1488337/151‑1 (MQ=255)

                             gTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGa                                                                                                                   <  1:19708/151‑1 (MQ=255)

                               tAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGAc                                                                                                                  >  2:1707549/1‑150 (MQ=255)

                               tAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGATGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGAc                                                                                                                  >  1:1615996/1‑150 (MQ=255)

                                aaTTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACAt                                                                                                                <  1:1020265/151‑1 (MQ=255)

                                 aTTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACAt                                                                                                                >  2:1657105/1‑150 (MQ=255)

                                   tcatcaTTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACt                                                                                                             >  1:1539980/1‑151 (MQ=255)

                                    catcatTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACtt                                                                                                            <  1:1431083/151‑1 (MQ=255)

                                    catcatTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACt                                                                                                             <  1:1482287/150‑1 (MQ=255)

                                      tcatTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTc                                                                                                           >  1:41214/1‑150 (MQ=255)

                                           attaCATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGaa                                                                                                     <  2:390412/151‑1 (MQ=255)

                                              aCATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCt                                                                                                  <  2:540945/151‑1 (MQ=255)

                                                   atcatTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTtgt                                                                                             <  1:1284158/151‑1 (MQ=255)

                                                     catTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGa                                                                                           <  1:507520/151‑1 (MQ=255)

                                                        tGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTc                                                                                        >  2:479494/1‑151 (MQ=255)

                                                         gTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTc                                                                                        >  2:1392540/1‑150 (MQ=255)

                                                         gTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCa                                                                                       <  1:1449774/151‑1 (MQ=255)

                                                         gTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCa                                                                                       <  1:1042254/151‑1 (MQ=255)

                                                         gTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCa                                                                                       <  2:464877/151‑1 (MQ=255)

                                                           aaaTAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAgg                                                                                     >  2:585680/1‑151 (MQ=255)

                                                             ataatTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGTGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGc                                                                                    >  1:501136/1‑150 (MQ=255)

                                                             ataatTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCa                                                                                   <  2:85434/151‑1 (MQ=255)

                                                                     attaaCTTCCATAACATTAAAATATCTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTAt                                                                           <  1:659835/151‑1 (MQ=255)

                                                                      ttaaCTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCGGGCAACCAGTATg                                                                          >  2:449977/1‑151 (MQ=255)

                                                                       taaCTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGc                                                                         >  1:1095681/1‑151 (MQ=255)

                                                                             ccATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAAc                                                                   <  1:1493175/151‑1 (MQ=255)

                                                                             ccATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAAc                                                                   <  2:907875/151‑1 (MQ=255)

                                                                              cATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACg                                                                  >  2:1679197/1‑151 (MQ=255)

                                                                              cATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACg                                                                  >  1:1217962/1‑151 (MQ=255)

                                                                                tAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGac                                                                >  1:873998/1‑151 (MQ=255)

                                                                                tAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGa                                                                 <  1:1359423/150‑1 (MQ=255)

                                                                                       aaaaTATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGAtt                                                         <  1:1246191/151‑1 (MQ=255)

                                                                                         aaTATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGAttt                                                        >  1:320307/1‑150 (MQ=255)

                                                                                         aaTATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGAttt                                                        >  2:1369408/1‑150 (MQ=255)

                                                                                            atGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACGTACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAAc                                                     <  1:1143959/150‑1 (MQ=255)

                                                                                             tGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAAct                                                    <  2:1657992/150‑2 (MQ=255)

                                                                                              gTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAg                                                   <  1:415915/150‑1 (MQ=255)

                                                                                              gTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAg                                                   <  1:1308279/150‑1 (MQ=255)

                                                                                              gTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGt                                                  <  2:906411/151‑1 (MQ=255)

                                                                                                aTCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCg                                                >  1:940119/1‑151 (MQ=255)

                                                                                                aTCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCg                                                >  1:740691/1‑151 (MQ=255)

                                                                                                aTCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCG‑CTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCg                                                <  2:272735/150‑1 (MQ=255)

                                                                                                 tCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGc                                               >  2:1238840/1‑151 (MQ=255)

                                                                                                 tCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGc                                               >  1:764293/1‑151 (MQ=255)

                                                                                                  ccACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCa                                              <  1:602389/151‑1 (MQ=255)

                                                                                                     cTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATg                                            >  2:1288293/1‑150 (MQ=255)

                                                                                                     cTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATg                                            >  2:1256022/1‑150 (MQ=255)

                                                                                                      tGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGAc                                          >  1:678119/1‑151 (MQ=255)

                                                                                                         cGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCg                                       <  1:76740/151‑1 (MQ=255)

                                                                                                            ttttttACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTAt                                    >  1:796420/1‑151 (MQ=255)

                                                                                                            ttttttACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTAt                                    >  2:1043236/1‑151 (MQ=255)

                                                                                                              ttttACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCg                                  >  2:1608009/1‑151 (MQ=255)

                                                                                                               tttACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGt                                 >  2:1575574/1‑151 (MQ=255)

                                                                                                                ttACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGt                                 <  2:713034/150‑1 (MQ=255)

                                                                                                                ttACATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGt                                 >  2:1342602/1‑150 (MQ=255)

                                                                                                                  aCATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACt                              <  2:433176/151‑1 (MQ=255)

                                                                                                                  aCATAACGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACt                              <  1:592849/151‑1 (MQ=255)

                                                                                                                        cGAAGAATTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCtcat                        <  2:327026/151‑1 (MQ=255)

                                                                                                                           agaaTTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAg                     <  2:1230309/151‑1 (MQ=255)

                                                                                                                             aaTTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCt                   <  1:1187786/151‑1 (MQ=255)

                                                                                                                             aaTTGACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCt                   >  2:73823/1‑151 (MQ=255)

                                                                                                                                 gACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTa                <  2:989800/150‑1 (MQ=255)

                                                                                                                                 gACCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTa                <  2:1076693/150‑1 (MQ=255)

                                                                                                                                  aCCATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTAtc              <  2:1204411/151‑1 (MQ=255)

                                                                                                                                   ccATTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTAtct             >  1:326795/1‑151 (MQ=255)

                                                                                                                                     aTTTTGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTAtcttc           >  1:986797/1‑151 (MQ=255)

                                                                                                                                      ttttGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTAtcttct          >  2:783811/1‑151 (MQ=255)

                                                                                                                                        ttGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTAtcttctt         <  1:213175/150‑1 (MQ=255)

                                                                                                                                         tGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTAtcttcttct       >  2:639710/1‑151 (MQ=255)

                                                                                                                                         tGTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTAtcttcttct       >  2:321943/1‑151 (MQ=255)

                                                                                                                                          gTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTAtcttcttct       <  1:485723/150‑1 (MQ=255)

                                                                                                                                          gTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTATcttcttctt      <  2:1397680/151‑1 (MQ=255)

                                                                                                                                          gTCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTATcttcttctt      <  1:1052974/151‑1 (MQ=255)

                                                                                                                                           tCCTGTTGCGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCttt     >  2:887473/1‑151 (MQ=255)

                                                                                                                                              tgttgcGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGc  >  2:673028/1‑151 (MQ=255)

                                                                                                                                               gttgcGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGc  <  1:665378/150‑1 (MQ=255)

CCTGATTAATGAATTATTACGTTTATCATGTTAATTCATCATTATTACATCATCATTGTAAATAATTAAATTAACTTCCATAACATTAAAATATGTATCCACTGACGCTTTTTTACATAACGAAGAATTGACCATTTTGTCCTGTTGTGCCTTAATGTAAGTACCGTCCACAGCGTGGGACATACTTCAAGGAACCTTTTGTGAGTCAGGCAACCAGTATGCGAAAACGACACCGATTTAACAGTCGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGC  >  NC_000913/2629090‑2629382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A