Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I3 R1 143 109.6 3434427 98.5% 3382910 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,328,816 (A)7→6 intergenic (‑101/‑147) greA ← / → dacB transcript cleavage factor/D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,328,8100A.99.1% 540.7 / ‑1.7 112intergenic (‑95/‑153)greA/dacBtranscript cleavage factor/D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (60/51);  total (60/52)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 125 total aligned reads displayed.

CCAGCTCTTCGCGTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCAAAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCAGGTTGTTAGCGC  >  NC_000913/3328661‑3328956
                                                                                                                                                     |                                                                                                                                                  
ccAGCTCTTCGCGTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCaa                                                                                                                                                   <  2:960233/151‑1 (MQ=255)
ccAGCTCTTCGCGTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCa                                                                                                                                                    >  2:1220109/1‑150 (MQ=255)
 cAGCTCTTCGCGTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAATCaa                                                                                                                                                   >  2:1457832/1‑150 (MQ=255)
   gCTCTTCGCGTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCaaaaa                                                                                                                                                <  2:1046330/151‑1 (MQ=255)
       ttCGCGTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCaaaaaatac                                                                                                                                            >  2:1482365/1‑148 (MQ=255)
        tCGCGTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCaaaaaatacc                                                                                                                                           >  2:1470176/1‑147 (MQ=255)
        tCGCGTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCaaaaaatac                                                                                                                                            <  2:79500/150‑4 (MQ=255)
        tCGCGTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCaaaaaat                                                                                                                                              <  1:89284/148‑2 (MQ=255)
         cgcgTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCg                                                                                                                                         >  1:492561/1‑151 (MQ=255)
          gcgTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGa                                                                                                                                        <  1:82094/151‑1 (MQ=255)
          gcgTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGa                                                                                                                                        <  1:1381538/151‑1 (MQ=255)
              aaTTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCg                                                                                                                                    <  1:1520788/151‑1 (MQ=255)
               aTTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCgg                                                                                                                                   <  2:1155245/151‑1 (MQ=255)
                tttttCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCggg                                                                                                                                  >  1:1037154/1‑151 (MQ=255)
                 ttttCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCggg                                                                                                                                  <  2:152992/150‑1 (MQ=255)
                  tttCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGt                                                                                                                                 <  2:926559/150‑1 (MQ=255)
                  tttCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGt                                                                                                                                 <  2:666319/150‑1 (MQ=255)
                   ttCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTa                                                                                                                                <  2:528711/150‑1 (MQ=255)
                   ttCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTa                                                                                                                                <  1:1544124/150‑1 (MQ=255)
                     cAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCAAAAAAATACCGACCCGGGTACa                                                                                                                              <  2:139156/151‑1 (MQ=255)
                     cAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACa                                                                                                                              >  1:1069560/1‑150 (MQ=255)
                      aGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACa                                                                                                                              >  2:1407020/1‑149 (MQ=255)
                       gcgcCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGt                                                                                                                           >  2:46483/1‑151 (MQ=255)
                       gcgcCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGt                                                                                                                           <  2:1462926/151‑1 (MQ=255)
                        cgccgcGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCg                                                                                                                                         <  1:282144/136‑1 (MQ=255)
                        cgccgcGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTc                                                                                                                          >  1:1024164/1‑151 (MQ=255)
                         gccgcGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTcc                                                                                                                         <  2:665291/151‑1 (MQ=255)
                         gccgcGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTcc                                                                                                                         >  1:417732/1‑151 (MQ=255)
                         gccgcGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTcc                                                                                                                         <  1:1330503/151‑1 (MQ=255)
                          ccgcGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTccc                                                                                                                        <  2:1229093/151‑1 (MQ=255)
                           cgcgTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCa                                                                                                                       <  1:1193393/151‑1 (MQ=255)
                           cgcgTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCa                                                                                                                       <  1:793170/151‑1 (MQ=255)
                            gcgTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAg                                                                                                                      <  1:1102134/151‑1 (MQ=255)
                             cgTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAgg                                                                                                                     >  1:144600/1‑151 (MQ=255)
                             cgTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAgg                                                                                                                     >  1:375613/1‑151 (MQ=255)
                              gTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGt                                                                                                                    >  1:1656279/1‑151 (MQ=255)
                              gTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGt                                                                                                                    <  2:188092/151‑1 (MQ=255)
                               tAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTc                                                                                                                   >  1:1682938/1‑151 (MQ=255)
                                 aGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTc                                                                                                                   >  2:1068396/1‑149 (MQ=255)
                                  ggTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGc                                                                                                                >  2:268437/1‑151 (MQ=255)
                                    tCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCt                                                                                                               >  2:456169/1‑150 (MQ=255)
                                     cATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTa                                                                                                              <  2:263018/150‑1 (MQ=255)
                                     cATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTAc                                                                                                             >  2:201759/1‑151 (MQ=255)
                                      aTCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACa                                                                                                            <  1:85316/151‑1 (MQ=255)
                                       tCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACa                                                                                                            >  1:19287/1‑150 (MQ=255)
                                        cGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACaa                                                                                                           <  2:507277/150‑1 (MQ=255)
                                           aaTAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTc                                                                                                        >  2:202518/1‑150 (MQ=255)
                                            aTAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTcac                                                                                                      >  2:756288/1‑151 (MQ=255)
                                                  tGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACAtttt                                                                                                 <  2:1538980/150‑1 (MQ=255)
                                                  tGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACAttt                                                                                                  <  1:1714378/149‑1 (MQ=255)
                                                   gCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTg                                                                                                >  1:965052/1‑150 (MQ=255)
                                                   gCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGa                                                                                               <  2:1602933/151‑1 (MQ=255)
                                                    cATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGAt                                                                                              <  2:1445118/151‑1 (MQ=255)
                                                     aTTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGAtt                                                                                             <  1:1107256/151‑2 (MQ=255)
                                                     aTTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGAt                                                                                              >  2:1035172/1‑150 (MQ=255)
                                                     aTTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGAt                                                                                              >  2:301423/1‑150 (MQ=255)
                                                     aTTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGAt                                                                                              >  2:330004/1‑150 (MQ=255)
                                                        tGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGt                                                                                           <  2:240032/150‑1 (MQ=255)
                                                            ataCCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCAttt                                                                                      >  1:592434/1‑151 (MQ=255)
                                                             taCCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCAtttt                                                                                     >  2:1052454/1‑151 (MQ=255)
                                                                  ctTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCt                                                                                >  2:120946/1‑151 (MQ=255)
                                                                     gAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGa                                                                              >  1:466335/1‑150 (MQ=255)
                                                                      aaTATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAg                                                                            <  2:356541/151‑1 (MQ=255)
                                                                       atatTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAg                                                                            >  2:553597/1‑150 (MQ=255)
                                                                         atTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTc                                                                         <  2:915974/151‑1 (MQ=255)
                                                                          ttCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTcc                                                                        >  2:422599/1‑151 (MQ=255)
                                                                           tCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTcc                                                                        <  1:957595/150‑1 (MQ=255)
                                                                              tGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGa                                                                     >  2:908341/1‑150 (MQ=255)
                                                                                aTAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAgg                                                                  >  1:1394933/1‑151 (MQ=255)
                                                                                aTAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAgg                                                                  >  2:1126045/1‑151 (MQ=255)
                                                                                   gggCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTc                                                               <  2:849938/151‑1 (MQ=255)
                                                                                      cAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATc                                                            <  2:932941/151‑1 (MQ=255)
                                                                                       aaGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCg                                                           <  1:17642/151‑1 (MQ=255)
                                                                                             tCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGttt                                                        <  2:918226/148‑1 (MQ=255)
                                                                                             tCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGttt                                                        <  1:415995/148‑1 (MQ=255)
                                                                                               acccacccCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACttt                                                   >  1:1495641/1‑151 (MQ=255)
                                                                                                 ccacccCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTtat                                                 <  1:1633400/151‑1 (MQ=255)
                                                                                                  cacccCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTtat                                                 >  1:1093037/1‑150 (MQ=255)
                                                                                                   acccCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTtat                                                 >  1:1447524/1‑149 (MQ=255)
                                                                                                     cccGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAggg                                             <  1:1610252/151‑1 (MQ=255)
                                                                                                       cGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCg                                           >  2:1604682/1‑151 (MQ=255)
                                                                                                          cTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTg                                        >  1:1179738/1‑151 (MQ=255)
                                                                                                              tCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTgcgc                                     <  1:698744/150‑1 (MQ=255)
                                                                                                               cGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCg                                   <  1:908273/151‑1 (MQ=255)
                                                                                                                ggCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCg                                   >  2:902193/1‑150 (MQ=255)
                                                                                                                    tGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCgtagt                               >  1:1533241/1‑150 (MQ=255)
                                                                                                                     gCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTAt                             >  1:1145105/1‑151 (MQ=255)
                                                                                                                        cGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGAc                          <  1:1220015/151‑1 (MQ=255)
                                                                                                                        cGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGAc                          >  2:1147501/1‑151 (MQ=255)
                                                                                                                        cGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGAc                          <  1:1042280/151‑1 (MQ=255)
                                                                                                                         ggCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACg                         <  2:662477/151‑1 (MQ=255)
                                                                                                                          gCATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACgg                        >  1:1639099/1‑151 (MQ=255)
                                                                                                                           cATAACTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGc                       >  2:1289136/1‑151 (MQ=255)
                                                                                                                              aaCTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCg                    <  2:1145330/151‑1 (MQ=255)
                                                                                                                               aCTCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCg                    >  2:232941/1‑150 (MQ=255)
                                                                                                                                 tCCTTACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGAtt                 >  2:778829/1‑151 (MQ=255)
                                                                                                                                     tACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTcc               >  2:1517873/1‑149 (MQ=255)
                                                                                                                                     tACCGGGACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCa              <  2:883758/150‑1 (MQ=255)
                                                                                                                                         gggACCAGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCAGgttg         >  1:855711/1‑151 (MQ=255)
                                                                                                                                               aGAAGC‑AAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCAGGTTGTTAgcg   >  2:182929/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCAGCTCTTCGCGTAATTTTTCAGCGCCGCGTAAGGTCATCGGAATAGCTTGCATTTGTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCAAAAAAATACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCAGGTTGTTAGCGC  >  NC_000913/3328661‑3328956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A