Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I3 R1	143	109.6	3434427	98.5%	3382910	150.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,421,201	G→A	T356T (ACG→ACA)	yhdX →	putative amino acid ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,421,201	0	G	A	92.2%	299.9 / ‑1.7	91	T356T (ACG→ACA)	yhdX	putative amino acid ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (1/6); new base A (57/26); total (59/32)
Fisher's exact test for biased strand distribution p-value = 7.49e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGGTCATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCA  >  NC_000913/3421053‑3421347

gcCAGGTCATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACagg                                                                                                                                                  <  2:1435200/151‑1 (MQ=255)

  cAGGTCATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGc                                                                                                                                                >  1:1480715/1‑151 (MQ=255)

   aGGTCATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGc                                                                                                                                                >  2:387727/1‑150 (MQ=255)

   aGGTCATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGc                                                                                                                                                >  2:1635621/1‑150 (MQ=255)

   aGGTCATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCa                                                                                                                                               <  2:1565474/151‑1 (MQ=255)

    ggTCATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCaa                                                                                                                                              <  1:1183019/151‑1 (MQ=255)

    ggTCATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCa                                                                                                                                               >  2:598562/1‑150 (MQ=255)

      tCATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGc                                                                                                                                            >  2:829204/1‑151 (MQ=255)

       cATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCATTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGcc                                                                                                                                           >  2:95251/1‑151 (MQ=255)

       cATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCCGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGcc                                                                                                                                           >  1:383847/1‑151 (MQ=255)

        attattCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGcc                                                                                                                                           >  2:1602804/1‑150 (MQ=255)

         ttattCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGcc                                                                                                                                           <  1:127846/149‑1 (MQ=255)

         ttattCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCAt                                                                                                                                         >  2:576276/1‑151 (MQ=255)

         ttattCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCAt                                                                                                                                         >  2:1528487/1‑151 (MQ=255)

                 cAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACg                                                                                                                                  >  2:1289251/1‑150 (MQ=255)

                   ggCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATa                                                                                                                               <  2:1355757/151‑1 (MQ=255)

                    gCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGAt                                                                                                                                >  2:806510/1‑149 (MQ=255)

                    gCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAg                                                                                                                              <  1:1535522/151‑1 (MQ=255)

                     cATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGc                                                                                                                             >  1:1246996/1‑151 (MQ=255)

                      aTTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGcc                                                                                                                            >  2:546499/1‑151 (MQ=255)

                          cGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATg                                                                                                                         >  1:1001998/1‑150 (MQ=255)

                              tGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCAt                                                                                                                    >  1:77742/1‑151 (MQ=255)

                                 ttattCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTc                                                                                                                 >  2:1493819/1‑151 (MQ=255)

                                    ttCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGt                                                                                                              >  2:276957/1‑151 (MQ=255)

                                    ttCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGt                                                                                                              >  1:635934/1‑151 (MQ=255)

                                      cccccGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGt                                                                                                              <  2:932722/146‑1 (MQ=255)

                                      ccACCGTTACCCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTc                                                                                                             <  1:877157/150‑1 (MQ=255)

                                        aCCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTAt                                                                                                          >  2:538955/1‑151 (MQ=255)

                                          cGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCt                                                                                                        <  2:1326272/151‑1 (MQ=255)

                                              aaCCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAtt                                                                                                    >  2:1177758/1‑151 (MQ=255)

                                              aaCCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAtt                                                                                                    >  2:96167/1‑151 (MQ=255)

                                              aaCCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAtt                                                                                                    >  1:342705/1‑151 (MQ=255)

                                               aCCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGTCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAtt                                                                                                    <  1:670405/150‑1 (MQ=255)

                                                ccagccagTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTa                                                                                                   <  1:14302/150‑1 (MQ=255)

                                                 cagccagTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATc                                                                                                 <  2:959769/151‑1 (MQ=255)

                                                   gccagTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAg                                                                                               <  1:296425/151‑1 (MQ=255)

                                                    ccagTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGc                                                                                              >  2:1246043/1‑151 (MQ=255)

                                                     cagTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGcc                                                                                             >  1:1607408/1‑151 (MQ=255)

                                                       gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTg                                                                                           <  1:207124/151‑1 (MQ=255)

                                                         aTCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGAc                                                                                         >  2:1592017/1‑151 (MQ=255)

                                                          tctcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACt                                                                                        >  2:844617/1‑151 (MQ=255)

                                                            tcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAt                                                                                      >  1:1304586/1‑151 (MQ=255)

                                                            tcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAt                                                                                      >  1:1128771/1‑151 (MQ=255)

                                                              aaCATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                                                                                    <  2:1685750/151‑1 (MQ=255)

                                                               aCATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtctc                                                                                   >  2:1406274/1‑151 (MQ=255)

                                                                cATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtctc                                                                                   <  1:611739/150‑1 (MQ=255)

                                                                 aTCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgc                                                                                 >  1:281571/1‑151 (MQ=255)

                                                                    gTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctgc                                                                              >  2:603154/1‑151 (MQ=255)

                                                                     tCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctgct                                                                             >  2:915267/1‑151 (MQ=255)

                                                                     tCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctgct                                                                             >  1:1573042/1‑151 (MQ=255)

                                                                       aaaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGctgctg                                                                            >  1:117007/1‑150 (MQ=255)

                                                                       aaaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGctgctg                                                                            >  1:1271398/1‑150 (MQ=255)

                                                                           aCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgatga                                                                        >  2:91291/1‑150 (MQ=255)

                                                                           aCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGaa                                                                       <  2:850512/151‑1 (MQ=255)

                                                                             tcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAat                                                                      >  1:739490/1‑150 (MQ=255)

                                                                              cctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAatat                                                                    >  1:1495642/1‑151 (MQ=255)

                                                                               ctctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAatat                                                                    >  1:975533/1‑150 (MQ=255)

                                                                                   ctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCt                                                                  <  2:1454344/148‑1 (MQ=255)

                                                                                     tGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtata                                                               <  2:14430/149‑1 (MQ=255)

                                                                                     tGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtat                                                                <  1:1702880/148‑1 (MQ=255)

                                                                                     tGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtat                                                                <  1:1066585/148‑1 (MQ=255)

                                                                                       ccgccgCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAccg                                                           >  1:1579033/1‑151 (MQ=255)

                                                                                       ccgccgCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAccg                                                           <  1:391607/151‑1 (MQ=255)

                                                                                          ccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAccgccg                                                        >  1:313052/1‑151 (MQ=255)

                                                                                             cTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCAt                                                     >  2:18757/1‑151 (MQ=255)

                                                                                                    cTACCCCGATTTGTTTTCGTTGTTTGCCGCCCCCGTGCTAAATCAGACGGGGAAACCCATCGAGACGATAGCCATGACCATGTCGTTCTATCTGTTTACCAGCCTGACTATCTCGCTGCTGATAAATATCTATAACCGCCGCATCGCGAt                                               <  2:852388/150‑1 (MQ=255)

                                                                                                      aTCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGt                                            >  1:293464/1‑151 (MQ=255)

                                                                                                      aTCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGt                                            >  1:1100194/1‑151 (MQ=255)

                                                                                                        cccGATATGGTTTCGTTGTTTGCCGCCCCGGTGCTGAATCAGACGGGGCACGCCATGGAGACGATGGCCATGACCATGCCGTTCTTTCTGATTATCAGCCTGACTCTCTCGCTGCTGATAAATATCTATAACCGCCGCATCGCGATCGt                                            <  1:1513317/149‑1 (MQ=255)

                                                                                                        cccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTg                                          >  2:1418809/1‑151 (MQ=255)

                                                                                                         cggATTTGGTTCCGTTTTTTGCGGCCCCCGGGCTAAACCAGACAGGCAAACCCACGGAAACGATGCCAATGCCCATGTCGTTTTATTTGATAACCACCCTGCCTTTCCCGCTGCGGATAAATTTTTATACCCGCCGCATCGCGATCGTTGa                                         <  1:1436336/149‑1 (MQ=255)

                                                                                                                  tttCGCTTTTTGCCGCCCCCGTGCTAAATCAGACAGGGAAACCCATCGAGACGATAGCCATGCCCATGTCGTTTTTTCTGATAATCAGCCTGCTTATCTCGCTGTTGATAAATATTTATAACCGCCGCATCGCGATCGTTGAACGCTa                                   <  1:53926/148‑1 (MQ=255)

                                                                                                                  tttCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAgg                                >  1:1565224/1‑151 (MQ=255)

                                                                                                                    tCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAgg                                >  1:242735/1‑149 (MQ=255)

                                                                                                                    tCGCTGTTTGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAgg                                >  1:836625/1‑149 (MQ=255)

                                                                                                                        tGTTGGCCGCCCCCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTTTATCTAATTATCACCCTGACTTTCTCGCTGCTGAAAAATATTTAAAACCGCCGCATCGCGATCGt                                            <  1:598508/133‑1 (MQ=255)

                                                                                                                          tttCCCGCCCCCGTGTTGAACCAAACGGGCCAACCCCGCGAGACGATAGCCATGCCCATGTCGGTCTATCTATTTATCACCCTGACTTTCTCCCGGCTGATAAATATCTAAAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCatg                         <  1:416802/150‑1 (MQ=255)

                                                                                                                            tGCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCatgat                       >  1:630907/1‑150 (MQ=255)

                                                                                                                             gCCGGCACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCatgatg                      >  2:1641806/1‑150 (MQ=255)

                                                                                                                                  cACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACaaa                 >  1:1008854/1‑150 (MQ=255)

                                                                                                                                  cACCGTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACaaa                 >  1:664093/1‑150 (MQ=255)

                                                                                                                                      gTGCTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTAt            >  1:1022173/1‑151 (MQ=255)

                                                                                                                                        gCTAAACCAGACGGGGCAACCAACCGAGACGATAGCCATGCCCTTGTCGTTCTATCTGATTACCACCCTGACTTTCTCGCTGTTGATAAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTAtt           <  2:1128857/150‑1 (MQ=255)

                                                                                                                                         cTGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGc         >  2:1692934/1‑151 (MQ=255)

                                                                                                                                          tGAATCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCt        >  1:1392060/1‑151 (MQ=255)

                                                                                                                                            aaTCAGACGGGGAACGCCATGGAGCCAATACCAATGACCTGCTGGGTCTATTTAATTACCACCCTCCCTATCTCGCTGCTAATAAATATCTAAAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTg       <  2:1247093/150‑1 (MQ=255)

                                                                                                                                             ataaGACAGGGCAAGCCTTCGAGACGATAGCCATGACCATGTCGGTTTATCTAATTATCAGCCTGACTATCTCGCTGCTGATAAATATTTAAAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGtc     <  2:1611808/148‑1 (MQ=255)

                                                                                                                                             accAGACGGGAAAACCCATGGAGACGATAGCCATGCCCATGTGGTTCTTTTTGATTACCAGCCTGATTATCTCGCTGCTGATAAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGtc     <  2:117015/149‑1 (MQ=255)

                                                                                                                                              tCAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGtct    >  2:173398/1‑151 (MQ=255)

                                                                                                                                               cAGACAGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGtctc   >  1:280321/1‑151 (MQ=255)

                                                                                                                                                aGACCGGGAAAGCCATCTAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGtctc   >  1:1359881/1‑150 (MQ=255)

                                                                                                                                                 gACAGGGCAAGCCAGCGAGACGATAGCCAGGACCATGTCGTTTTTTCTGTTTATCACCCTGACTATCTCGCTGCTAATAAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCa  <  2:989189/150‑1 (MQ=255)

GCCAGGTCATTATTCCCCAGGCATTGCGGGTGATTATTCCACCGTTAACCAGCCAGTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATCGCGATCGTTGAACGCTAAGGAGTCATGATGACAAAAGTATTGCTGTCTCA  >  NC_000913/3421053‑3421347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A