Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I3 R1 143 109.6 3434427 98.5% 3382910 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,964,687 A→G V315A (GTT→GCT)  rhlB ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,964,6870AG97.6% 442.5 / 1.4 127V315A (GTT→GCT) rhlBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (65/59);  total (67/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

Only 100 of 127 total aligned reads displayed.

ATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTT  >  NC_000913/3964539‑3964834
                                                                                                                                                    |                                                                                                                                                   
aTACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCg                                                                                                                                                   >  2:1333673/1‑151 (MQ=255)
 tACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTTTGTCACTGCCGGATTATGCAAACCACGCGCGGCAGCGt                                                                                                                                                  >  1:953278/1‑151 (MQ=255)
  aCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGt                                                                                                                                                  <  2:1699475/150‑1 (MQ=255)
  aCTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTc                                                                                                                                                 <  1:1041672/151‑1 (MQ=255)
    tctTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCgg                                                                                                                                               >  2:825343/1‑151 (MQ=255)
     ctTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCgg                                                                                                                                               >  2:1638635/1‑150 (MQ=255)
      ttCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTg                                                                                                                                             >  1:326675/1‑151 (MQ=255)
       tCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTgg                                                                                                                                            >  1:834680/1‑151 (MQ=255)
       tCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTg                                                                                                                                             <  1:228069/150‑1 (MQ=255)
        cacaCGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTgg                                                                                                                                            <  1:1213878/150‑1 (MQ=255)
          cacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCaa                                                                                                                                         <  2:1392331/151‑1 (MQ=255)
          cacGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCa                                                                                                                                          >  1:638513/1‑150 (MQ=255)
            cGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAAcc                                                                                                                                       <  2:94174/151‑1 (MQ=255)
              ccAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAg                                                                                                                                     <  1:953253/151‑1 (MQ=255)
              ccAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAg                                                                                                                                     <  2:820816/151‑1 (MQ=255)
               cAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAAcc                                                                                                                                       >  2:446401/1‑148 (MQ=255)
               cAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGa                                                                                                                                    <  1:585102/151‑1 (MQ=255)
                aGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGaa                                                                                                                                   <  2:1282787/151‑1 (MQ=255)
                aGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGa                                                                                                                                    >  1:763484/1‑150 (MQ=255)
                  gCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAata                                                                                                                                 <  1:175522/151‑1 (MQ=255)
                        agagTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCCGCTTCGGTGGCAACCAGAATATCCAg                                                                                                                            <  1:1704773/150‑1 (MQ=255)
                         gagTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGAt                                                                                                                          <  1:1545726/151‑1 (MQ=255)
                           gTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCg                                                                                                                        <  1:168001/151‑1 (MQ=255)
                                 gCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACgg                                                                                                                  <  2:213890/151‑1 (MQ=255)
                                    tGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGt                                                                                                                <  1:743867/150‑1 (MQ=255)
                                     gcgcCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTa                                                                                                               >  1:743827/1‑150 (MQ=255)
                                          tGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATtc                                                                                                          >  2:1050170/1‑150 (MQ=255)
                                          tGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATtc                                                                                                          >  2:1306675/1‑150 (MQ=255)
                                           gcgcGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATtca                                                                                                         <  1:551694/150‑1 (MQ=255)
                                              cgACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATtcatca                                                                                                      >  1:538059/1‑150 (MQ=255)
                                                 ccTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGaa                                                                                                  <  2:309746/151‑1 (MQ=255)
                                                  cTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAAt                                                                                                 >  1:1083940/1‑151 (MQ=255)
                                                   tGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAAt                                                                                                 >  1:569470/1‑150 (MQ=255)
                                                    gTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATAc                                                                                               <  2:1297914/151‑1 (MQ=255)
                                                      aCGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGc                                                                                             >  2:1139669/1‑151 (MQ=255)
                                                       cGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCa                                                                                            <  2:1180997/151‑1 (MQ=255)
                                                           cAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACg                                                                                        <  1:1089957/151‑1 (MQ=255)
                                                              tACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGTAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGttt                                                                                     >  1:164008/1‑151 (MQ=255)
                                                              tACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGttt                                                                                     >  2:562548/1‑151 (MQ=255)
                                                              tACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGttt                                                                                     >  1:529163/1‑151 (MQ=255)
                                                               aCGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGttt                                                                                     <  2:1309142/150‑1 (MQ=255)
                                                                cGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGttttt                                                                                   >  2:1369575/1‑151 (MQ=255)
                                                                cGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGttttt                                                                                   <  2:727568/151‑1 (MQ=255)
                                                                 ggTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGtttttt                                                                                  <  1:756344/151‑1 (MQ=255)
                                                                 ggTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGtttttt                                                                                  <  1:839644/151‑1 (MQ=255)
                                                                 ggTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGttttt                                                                                   <  1:1161237/150‑1 (MQ=255)
                                                                     aaCGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTgc                                                                              <  2:137456/151‑1 (MQ=255)
                                                                      aCGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTgcg                                                                             >  1:719384/1‑151 (MQ=255)
                                                                      aCGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTgcg                                                                             >  2:902017/1‑151 (MQ=255)
                                                                       cGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTgcgc                                                                            >  1:843079/1‑151 (MQ=255)
                                                                       cGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTgcgc                                                                            >  1:1056093/1‑151 (MQ=255)
                                                                       cGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTgcgc                                                                            <  2:83871/151‑1 (MQ=255)
                                                                        gTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGcgcg                                                                           >  1:1170434/1‑151 (MQ=255)
                                                                          aaTCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGAc                                                                         <  2:464650/151‑1 (MQ=255)
                                                                               tCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACa                                                                        <  1:1497258/147‑1 (MQ=255)
                                                                               tCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACAt                                                                       <  2:326701/148‑1 (MQ=255)
                                                                               tCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATc                                                                      <  1:44975/149‑1 (MQ=255)
                                                                               tCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGc                                                                    >  1:1591391/1‑151 (MQ=255)
                                                                                cacaGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGcc                                                                   >  1:1559911/1‑151 (MQ=255)
                                                                                cacaGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGcc                                                                   >  2:1619139/1‑151 (MQ=255)
                                                                                cacaGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGcc                                                                   >  1:1139988/1‑151 (MQ=255)
                                                                                 acaGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCt                                                                  <  2:608258/151‑1 (MQ=255)
                                                                                     tCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTc                                                               >  1:1237436/1‑150 (MQ=255)
                                                                                      cATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCaa                                                             <  1:706795/151‑1 (MQ=255)
                                                                                      cATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCa                                                              >  2:1280425/1‑150 (MQ=255)
                                                                                         cGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATa                                                           <  2:1171436/150‑1 (MQ=255)
                                                                                          gggTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATaa                                                          >  1:1457846/1‑150 (MQ=255)
                                                                                           ggTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAAc                                                        >  2:1049156/1‑151 (MQ=255)
                                                                                             tAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCg                                                      >  1:655242/1‑151 (MQ=255)
                                                                                              aaaTCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCg                                                      >  2:843757/1‑150 (MQ=255)
                                                                                                   gTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACg                                                 >  1:596649/1‑150 (MQ=255)
                                                                                                    tAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGAt                                               >  2:818131/1‑151 (MQ=255)
                                                                                                      gTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGa                                             <  1:1233268/151‑1 (MQ=255)
                                                                                                      gTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGa                                             <  2:1712189/151‑1 (MQ=255)
                                                                                                         aaaGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGAcc                                           <  1:446367/150‑1 (MQ=255)
                                                                                                              cGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATctgc                                     >  1:454576/1‑151 (MQ=255)
                                                                                                              cGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATctgc                                     >  1:913573/1‑151 (MQ=255)
                                                                                                               gTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATctgct                                    <  1:1623066/151‑1 (MQ=255)
                                                                                                               gTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATctgct                                    >  1:1454790/1‑151 (MQ=255)
                                                                                                                tGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATctgctg                                   >  2:44393/1‑151 (MQ=255)
                                                                                                                     cACTGCCGGAATATGCAAACCACGCGCGGCAGCATCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAg                               <  1:1188050/150‑1 (MQ=255)
                                                                                                                      aCTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGt                             <  2:774192/151‑1 (MQ=255)
                                                                                                                       cTGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTg                            >  2:1357736/1‑151 (MQ=255)
                                                                                                                        tGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTgg                           >  1:958894/1‑151 (MQ=255)
                                                                                                                        tGCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTgg                           >  2:929462/1‑151 (MQ=255)
                                                                                                                         gCCGGAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTgg                           <  1:562497/150‑1 (MQ=255)
                                                                                                                             gAATATGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGcccc                       >  1:520124/1‑150 (MQ=255)
                                                                                                                               atatGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGcccc                       >  1:408298/1‑148 (MQ=255)
                                                                                                                                 atGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGcccc                       >  1:345138/1‑146 (MQ=255)
                                                                                                                                 atGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtc                  <  2:887352/151‑1 (MQ=255)
                                                                                                                                 atGCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtc                  <  1:1050091/151‑1 (MQ=255)
                                                                                                                                   gCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtctc                <  2:223336/151‑1 (MQ=255)
                                                                                                                                   gCAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACACAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtctc                <  2:1035695/151‑1 (MQ=255)
                                                                                                                                    cAAACCACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATctct               >  2:1574905/1‑151 (MQ=255)
                                                                                                                                        ccACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTcac           <  2:1560006/151‑1 (MQ=255)
                                                                                                                                        ccACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTcac           <  2:715116/151‑1 (MQ=255)
                                                                                                                                         cACGCGCGGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTcaca          <  2:834742/151‑1 (MQ=255)
                                                                                                                                              gcgGCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGgt     >  2:1554900/1‑151 (MQ=255)
                                                                                                                                                ggCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGAAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGgtg    >  2:1320485/1‑150 (MQ=255)
                                                                                                                                                 gCAGCGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGtt  <  2:1180567/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTT  >  NC_000913/3964539‑3964834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A