Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F50 I1 R1 141 76.7 2402765 98.4% 2364320 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,084,257 T→C Y552C (TAC→TGC)  speA ← biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,084,2570TC100.0% 206.0 / NA 55Y552C (TAC→TGC) speAbiosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

GTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACT  >  NC_000913/3084109‑3084404
                                                                                                                                                    |                                                                                                                                                   
gTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCAc                                                                                                                                                   <  1:360482/151‑1 (MQ=255)
 tCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCAc                                                                                                                                                   >  1:236147/1‑150 (MQ=255)
   aGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTcc                                                                                                                                                >  2:1163313/1‑151 (MQ=255)
               ttctacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCggcattggcatt                                                                                                                                    >  2:841982/1‑151 (MQ=255)
                tctacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCggcattggcat                                                                                                                                     <  2:743669/149‑1 (MQ=255)
                  tacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAGACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTc                                                                                                                                 >  1:682263/1‑151 (MQ=255)
                  tacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTc                                                                                                                                 >  1:562416/1‑151 (MQ=255)
                   acGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGgcattggcattg                                                                                                                                   <  1:976121/148‑1 (MQ=255)
                    cGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGt                                                                                                                               <  2:984411/151‑1 (MQ=255)
                      cTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTgg                                                                                                                             >  2:610070/1‑151 (MQ=255)
                        aCCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGc                                                                                                                            >  2:124181/1‑150 (MQ=255)
                             cAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATAc                                                                                                                       <  1:1176525/150‑1 (MQ=255)
                                         acacGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAat                                                                                                          <  2:268054/151‑1 (MQ=255)
                                              tCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGt                                                                                                     >  1:434944/1‑151 (MQ=255)
                                                 aCCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGt                                                                                                  >  1:45632/1‑151 (MQ=255)
                                                    gCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGa                                                                                               <  1:482173/151‑1 (MQ=255)
                                                     cTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGa                                                                                               >  1:497144/1‑150 (MQ=255)
                                                        cGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAg                                                                                            <  1:519343/150‑1 (MQ=255)
                                                                 cGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCaga                                                                                  <  1:1106576/151‑1 (MQ=255)
                                                                      aGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTcac                                                                             >  2:51621/1‑151 (MQ=255)
                                                                        gTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAg                                                                           <  1:488140/151‑1 (MQ=255)
                                                                          tgtgCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAgg                                                                          >  1:991387/1‑150 (MQ=255)
                                                                             gCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAat                                                                      <  2:618794/151‑1 (MQ=255)
                                                                              cATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAata                                                                     <  1:802213/151‑1 (MQ=255)
                                                                               aTGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAatat                                                                    <  2:338365/151‑1 (MQ=255)
                                                                               aTGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAatat                                                                    >  2:879014/1‑151 (MQ=255)
                                                                                 gTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATc                                                                   <  2:1031214/150‑1 (MQ=255)
                                                                                      cGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCgagcag                                                             <  1:1156822/151‑1 (MQ=255)
                                                                                      cGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCcagcag                                                             <  1:841968/151‑1 (MQ=255)
                                                                                      cGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCcagcag                                                             <  1:550382/151‑1 (MQ=255)
                                                                                       gAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCagcagc                                                            <  1:200679/151‑1 (MQ=255)
                                                                                                cTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGcgcg                                                    >  2:768282/1‑150 (MQ=255)
                                                                                                  gATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGa                                                   <  1:518/149‑1 (MQ=255)
                                                                                                  gATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACg                                                 >  2:966359/1‑151 (MQ=255)
                                                                                                  gATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGAGTCTCTGGATCGCACTCCGGCAGTGGCATTGTCGTGGCAATACCGCCGCCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACg                                                 <  1:308430/151‑1 (MQ=255)
                                                                                                     atGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTc                                              <  2:972279/151‑1 (MQ=255)
                                                                                                       gcgcCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCg                                            >  2:832962/1‑151 (MQ=255)
                                                                                                            gACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACt                                       >  1:564/1‑151 (MQ=255)
                                                                                                             aCCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACtt                                      <  2:650060/151‑1 (MQ=255)
                                                                                                               cATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTg                                     >  2:637295/1‑150 (MQ=255)
                                                                                                                             gCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTcc                        >  2:731910/1‑149 (MQ=255)
                                                                                                                               agcggcggATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGc                     <  1:1158076/148‑1 (MQ=255)
                                                                                                                               aTCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCg                    <  1:1059043/151‑1 (MQ=255)
                                                                                                                                 cggcggATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACCGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGc                  >  1:989758/1‑151 (MQ=255)
                                                                                                                                 cggcggATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGc                  <  2:1139650/151‑1 (MQ=255)
                                                                                                                                   gcggATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGc                  >  1:1055522/1‑149 (MQ=255)
                                                                                                                                      gATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAAc             >  1:1077715/1‑151 (MQ=255)
                                                                                                                                       aTTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAAcc            >  1:204558/1‑151 (MQ=255)
                                                                                                                                       aTTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAAcc            <  1:525994/151‑1 (MQ=255)
                                                                                                                                       aTTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAAcc            <  2:1123612/151‑1 (MQ=255)
                                                                                                                                          ctctGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCggg         >  2:550821/1‑151 (MQ=255)
                                                                                                                                          ctctGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCggg         >  2:326852/1‑151 (MQ=255)
                                                                                                                                           tctGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCggg         >  2:565602/1‑150 (MQ=255)
                                                                                                                                                aTCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGaaca     >  1:498370/1‑149 (MQ=255)
                                                                                                                                                 tCGCACTCCGGCATTGGCATTGTCGGGGCAATACCGTCACCATCAATATAGTGGTCGATAGAACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACt  >  1:396587/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACT  >  NC_000913/3084109‑3084404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A