Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I1 R1	141	76.7	2402765	98.4%	2364320	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,463	T→C	G57G (GGA→GGG)	mnmG ←	5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,463	0	T	C	100.0%	179.4 / NA	48	G57G (GGA→GGG)	mnmG	5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/19); total (29/19)

CTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATG  >  NC_000913/3925316‑3925601

cTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGcccc                                                                                                                                         >  1:292727/1‑151 (MQ=255)

     ggTAGAGCACCCGCCCCGCCGGGGCGGGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCtt                                                                                                                                       <  1:370300/148‑1 (MQ=255)

         gagCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAAt                                                                                                                                 >  1:1108354/1‑150 (MQ=255)

           gCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATAcc                                                                                                                              >  1:85138/1‑151 (MQ=255)

              cccgccccGCCTGACCGCGGGAGGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATAccgc                                                                                                                            <  1:253572/144‑1 (MQ=255)

              cacGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATAccg                                                                                                                             <  2:869077/149‑1 (MQ=255)

                        cTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCggg                                                                                                                 >  2:884657/1‑151 (MQ=255)

                                      cgaaccccgccgGTCCTTTCTTTGCTTTTATTATCCTAAACT‑GGATCCCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCt                                                                                                    <  1:1191808/144‑1 (MQ=255)

                                       gcgAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGc                                                                                                  >  1:695925/1‑151 (MQ=255)

                                       gcgAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGc                                                                                                  >  2:76183/1‑151 (MQ=255)

                                                 gttcttttGCTGGCTTTTATTACCCAAAGCG‑GTATCCCCGCTTGATCGATCGCTTTCGCCATCAGCCCGCCGAGTGCCTCCCTTTCTTTTCCCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAgtgt                                                                                        <  1:370495/146‑1 (MQ=255)

                                                  ggtcTTTGCTTGGCTTTAATTCCCCAAAATGGGATCCCCGCTTGATCGTGCGCTTTCGCCACCAGACCGCCGAGTGCATCCACTTCTTTTACCAGACGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAgtgt                                                                                        <  1:612995/148‑1 (MQ=255)

                                                   tCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCg                                                                                      >  1:1137616/1‑151 (MQ=255)

                                                      tttGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGat                                                                                    >  1:555263/1‑150 (MQ=255)

                                                        tgcttgcGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATAtt                                                                                 >  1:514306/1‑151 (MQ=255)

                                                           ttgcGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATtgtg                                                                              >  2:219773/1‑151 (MQ=255)

                                                           ttgcGTTAAGTACCCTAAACG‑GGATCCCCGCTTGATCGATCGCTTTCGCCATCAGCCCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATtg                                                                                <  1:656323/149‑1 (MQ=255)

                                                             gcGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATtgtgtg                                                                            >  1:221328/1‑151 (MQ=255)

                                                               gTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTc                                                                          >  1:1007097/1‑151 (MQ=255)

                                                                   aGTATCCAAACCG‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCaa                                                                        <  2:1155129/149‑1 (MQ=255)

                                                                         cTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCag                                                                  >  1:173941/1‑149 (MQ=255)

                                                                          tAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGATGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGt                                                               >  1:787636/1‑151 (MQ=255)

                                                                            aaCT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCt                                                             >  2:937251/1‑151 (MQ=255)

                                                                               t‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGtt                                                          >  1:811508/1‑151 (MQ=255)

                                                                                 ggATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTg                                                         >  2:128984/1‑151 (MQ=255)

                                                                                    tACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGAcc                                                      >  2:291572/1‑151 (MQ=255)

                                                                                    tACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGAcc                                                      >  1:463204/1‑151 (MQ=255)

                                                                                     aCCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGAccc                                                     >  1:467333/1‑151 (MQ=255)

                                                                                      cccGCCTGATCGATCGCTTTCGCCATCAGCCCGCCGAGTGCATCCACTTCTTTTACCAGATCCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGAccc                                                     <  1:666373/150‑1 (MQ=255)

                                                                                      cccGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTCCCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGAcc                                                      <  1:871064/149‑1 (MQ=255)

                                                                                      cccGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGAccc                                                     >  2:1152764/1‑150 (MQ=255)

                                                                                      cccGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGAc                                                       <  2:173943/148‑1 (MQ=255)

                                                                                          ccTGATCGATCGCTTTCGCCATCAGACCGCCGATTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATAc                                                 <  2:5544/150‑1 (MQ=255)

                                                                                                cgatcgCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGcgccgc                                          >  1:796425/1‑151 (MQ=255)

                                                                                                cgatcgCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGcgccg                                           <  1:565573/150‑1 (MQ=255)

                                                                                                 gatcgCTTTCGCCATCAGCCCGCCGAGTGCATCCACTTCTTTTACCAGAGGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGcgccg                                           <  1:1152736/149‑1 (MQ=255)

                                                                                                     gCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGcc                                      >  1:271480/1‑150 (MQ=255)

                                                                                                       tttCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATc                                   >  2:141523/1‑151 (MQ=255)

                                                                                                         tCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATcgc                                 >  2:837664/1‑151 (MQ=255)

                                                                                                              aTCAGCCCGCCGAGTGCACCCTCTTCTTTTACCAGATCCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATc                                   <  1:436384/144‑1 (MQ=255)

                                                                                                               tCAGCCCGCCGAGTGCATCCACTTCTTTAACCAGATGCCCCTTCCAAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATcgc                                 <  2:917007/145‑1 (MQ=255)

                                                                                                                ccgACGGCCGGGTGCATCCCCTTCTTTGCCGAGATGCCCCCTCCCACTCCCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACGCATACGCGCCGCGGCCATCGCGGCCTc                           <  1:540880/148‑1 (MQ=255)

                                                                                                                cAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTc                           >  1:1017108/1‑150 (MQ=255)

                                                                                                                  gACCGCCGAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGt                        >  1:603998/1‑151 (MQ=255)

                                                                                                                    ccgccgAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTg                       <  2:349276/150‑1 (MQ=255)

                                                                                                                     cgccgAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGcc                     >  1:701499/1‑151 (MQ=255)

                                                                                                                         gAGTGCATCCACTTCTTTTACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGc                  >  1:469892/1‑150 (MQ=255)

                                                                                                                                      tcttTTCCCAGATGCCCCTCCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCa     <  2:42305/150‑1 (MQ=255)

                                                                                                                                        ttttACCAGATGCCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAatg  >  1:978056/1‑151 (MQ=255)

CTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATG  >  NC_000913/3925316‑3925601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A