Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,996	A→G	G269G (GGT→GGC)	afuC ←	CP4‑6 prophage; putative ferric transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,996	0	A	G	100.0%	127.9 / NA	35	G269G (GGT→GGC)	afuC	CP4‑6 prophage; putative ferric transporter subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/19); total (16/19)

CTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGAGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCACCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTAAAGATCCTGC  >  NC_000913/277849‑278140

cTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGccc                                                                                                                                               >  1:98323/1‑151 (MQ=255)

   cAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCtgt                                                                                                                                            <  1:265317/151‑1 (MQ=255)

   cAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCtgt                                                                                                                                            >  1:333350/1‑151 (MQ=255)

       aataTCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTAc                                                                                                                                        >  2:534676/1‑151 (MQ=255)

                       gCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGgcg                                                                                                                        >  1:283822/1‑151 (MQ=255)

                       gCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGgc                                                                                                                         <  2:97286/150‑1 (MQ=255)

                            tCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGc                                                                                                                    >  2:36591/1‑150 (MQ=255)

                               aCCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGc                                                                                                                    >  1:177288/1‑147 (MQ=255)

                                      cTTCATACTGCGGCCCCATATATGCGACATGGCGGGTCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACCCCGACCATCCCATCGCCCTGTGTACCAAAGTGCAGCGGGCGCTGCAGAGGATa                                                                                                            <  2:610357/148‑1 (MQ=255)

                                        tCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCg                                                                                                        <  1:493971/150‑1 (MQ=255)

                                          aTACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAg                                                                                                     <  2:133143/151‑1 (MQ=255)

                                              tGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGatat                                                                                                 >  2:130724/1‑151 (MQ=255)

                                                  gCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAAc                                                                                             <  1:213766/151‑1 (MQ=255)

                                                        tataGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATcc                                                                                       >  2:50380/1‑151 (MQ=255)

                                                          taGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGt                                                                                     >  2:98569/1‑151 (MQ=255)

                                                              cGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTATCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCt                                                                                 <  1:226646/151‑1 (MQ=255)

                                                               gACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTg                                                                                <  2:338362/151‑1 (MQ=255)

                                                                  aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                                                             <  2:65459/151‑1 (MQ=255)

                                                                  aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                                                             <  1:176271/151‑1 (MQ=255)

                                                                  aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                                                             <  1:406005/151‑1 (MQ=255)

                                                                        gATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGcc                                                                       <  1:98569/151‑1 (MQ=255)

                                                                         aTCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCg                                                                      >  1:615280/1‑151 (MQ=255)

                                                                            aCGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATGTCAACGTATCCGTCGCTGAAGGTTGCCGGGa                                                                   <  1:572949/151‑1 (MQ=255)

                                                                              gCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAAc                                                                 <  1:8124/151‑1 (MQ=255)

                                                                                      ggCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTg                                                           <  2:572740/149‑1 (MQ=255)

                                                                                                   gcgATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATaa                                              >  1:73038/1‑149 (MQ=255)

                                                                                                                  gATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCg                              <  2:121813/150‑1 (MQ=255)

                                                                                                                    tCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCggg                            >  2:382106/1‑150 (MQ=255)

                                                                                                                         tCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCgg                      >  1:206044/1‑151 (MQ=255)

                                                                                                                          ccGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCggg                     >  2:174494/1‑151 (MQ=255)

                                                                                                                              gcgcACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTgg                 >  2:64184/1‑151 (MQ=255)

                                                                                                                                   cacCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTa            <  2:192151/151‑1 (MQ=255)

                                                                                                                                   cacCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTa            <  2:631390/151‑1 (MQ=255)

                                                                                                                                      cGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTaa           >  1:357170/1‑149 (MQ=255)

                                                                                                                                             cccTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTAAAGATCCTGc  <  1:130727/151‑1 (MQ=255)

CTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGAGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCACCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTAAAGATCCTGC  >  NC_000913/277849‑278140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A