Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,523	A→G	intergenic (‑392/+60)	iscR ← / ← trmJ	isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,523	0	A	G	100.0%	158.4 / NA	47	intergenic (‑392/+60)	iscR/trmJ	isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/34); total (13/34)

CGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGC  >  NC_000913/2662380‑2662669

cgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCt                                                                                                                                             <  2:441154/151‑1 (MQ=255)

      aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCa                                                                                                                                        >  1:153150/1‑150 (MQ=255)

        cAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACt                                                                                                                                      <  1:374748/150‑1 (MQ=255)

                    gTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATaa                                                                                                                         <  1:605811/151‑1 (MQ=255)

                    gTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATaa                                                                                                                         <  2:558342/151‑1 (MQ=255)

                        gATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGGTCTGTTCACTGTAGGCCTGATaa                                                                                                                         >  1:143788/1‑147 (MQ=255)

                             ggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCAtt                                                                                                                 >  1:165186/1‑150 (MQ=255)

                              gcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAc                                                                                                               <  1:123290/151‑1 (MQ=255)

                               cgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAcg                                                                                                              <  2:107507/151‑1 (MQ=255)

                                  gAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAcgcg                                                                                                            <  1:370667/150‑1 (MQ=255)

                                   aaCGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGt                                                                                                           <  1:361814/150‑1 (MQ=255)

                                       ccTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCAt                                                                                                      <  2:356279/151‑1 (MQ=255)

                                           aTCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAg                                                                                                   <  2:421142/150‑1 (MQ=18)

                                           aTCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAgg                                                                                                  <  2:446561/151‑1 (MQ=38)

                                           aTCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAg                                                                                                   <  1:207478/150‑1 (MQ=38)

                                           aTCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAg                                                                                                   <  2:42903/150‑1 (MQ=38)

                                             ccGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCa                                                                                                <  2:600326/151‑1 (MQ=18)

                                                 ccTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACgg                                                                                            >  1:106494/1‑151 (MQ=25)

                                                            gTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTAtt                                                                                    <  2:459374/148‑1 (MQ=21)

                                                            gTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCg                                                                                  <  1:361003/150‑1 (MQ=25)

                                                                 cTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGGTATTCGGCCtt                                                                             >  2:342280/1‑150 (MQ=25)

                                                                     aGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTa                                                                         <  1:177933/150‑1 (MQ=255)

                                                                           gATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCttt                                                                    <  1:437889/149‑1 (MQ=255)

                                                                            aTAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTAtt                                                                 <  2:73122/151‑1 (MQ=255)

                                                                             tAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTAtt                                                                 >  2:246726/1‑150 (MQ=255)

                                                                                 aCGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctgct                                                            <  2:143785/151‑1 (MQ=255)

                                                                                  cGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctgctg                                                           <  2:317594/151‑1 (MQ=255)

                                                                                            gTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTAGCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa                                                 <  1:566012/151‑1 (MQ=255)

                                                                                             tCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa                                                 <  1:150888/150‑1 (MQ=255)

                                                                                              cGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAg                                                <  1:482640/150‑1 (MQ=255)

                                                                                                cATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGTCCTGCGGTTCTGTTCACGGTAGGCCTGATAAAAAGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCCTGTTACCTTTATTCTGCTTGTCAATAGAAGcc                                              >  2:479537/1‑150 (MQ=17)

                                                                                                   cAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGaa                                          <  2:14768/151‑1 (MQ=255)

                                                                                                   cAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGaa                                          <  2:445862/151‑1 (MQ=255)

                                                                                                          cGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCcgc                                    >  2:631403/1‑150 (MQ=255)

                                                                                                             cTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCgcgc                                  >  1:219642/1‑149 (MQ=255)

                                                                                                                 cGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGata                            <  1:503459/151‑1 (MQ=255)

                                                                                                                  ggATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGatat                           <  2:74084/151‑1 (MQ=255)

                                                                                                                    aTGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTc                         <  2:158608/151‑1 (MQ=255)

                                                                                                                        ggcgTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACt                     <  1:73136/151‑1 (MQ=255)

                                                                                                                         gcgTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTc                    <  2:420172/151‑1 (MQ=255)

                                                                                                                             gAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTgg                <  2:3288/151‑1 (MQ=255)

                                                                                                                             gAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTgg                >  1:227850/1‑151 (MQ=255)

                                                                                                                               aCGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCt              <  1:57110/151‑1 (MQ=255)

                                                                                                                                  ccTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTc           >  2:52629/1‑151 (MQ=255)

                                                                                                                                         cGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgc    <  1:478683/151‑1 (MQ=255)

                                                                                                                                           aCCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgcgc  >  1:125409/1‑151 (MQ=255)

                                                                                                                                            ccTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgcgc  >  1:48743/1‑150 (MQ=255)

CGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGC  >  NC_000913/2662380‑2662669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A