Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,978,727	T→C	N58S (AAT→AGT)	lysA ←	diaminopimelate decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,978,727	0	T	C	100.0%	144.2 / NA	38	N58S (AAT→AGT)	lysA	diaminopimelate decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/16); total (22/16)

CCTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATATTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGATCG  >  NC_000913/2978581‑2978870

ccTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGa                                                                                                                                             <  2:350743/151‑1 (MQ=255)

     tCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAgg                                                                                                                                        >  1:76474/1‑151 (MQ=255)

      cGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGc                                                                                                                                       >  1:155987/1‑151 (MQ=255)

         tAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCttt                                                                                                                                    >  2:343986/1‑151 (MQ=255)

              tCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCtgtg                                                                                                                               >  2:223112/1‑151 (MQ=255)

                      aaaaaCAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAgcg                                                                                                                        <  1:267032/150‑1 (MQ=255)

                       aaaaCAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAgcgc                                                                                                                       >  2:26442/1‑150 (MQ=255)

                        aaaCAATGTCATCGGGGTGCTTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTATCGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATCTGAATACTGTAACAGGCTTTCTGTGCAAAgcg                                                                                                                        <  2:459343/148‑1 (MQ=255)

                                  atcGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAAc                                                                                                           >  1:58320/1‑151 (MQ=255)

                                    cGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTg                                                                                                         >  2:399986/1‑151 (MQ=255)

                                           cGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAgc                                                                                                  <  1:110369/151‑1 (MQ=255)

                                               tGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCt                                                                                               <  2:178177/150‑1 (MQ=255)

                                                 cGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCa                                                                                            <  2:433770/151‑1 (MQ=255)

                                                  ggATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCa                                                                                            >  1:531932/1‑150 (MQ=255)

                                                       gTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTg                                                                                       <  1:223117/150‑1 (MQ=255)

                                                           cccGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCcgac                                                                                  >  2:568195/1‑151 (MQ=255)

                                                            ccgccgCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCcgacg                                                                                 >  1:463134/1‑151 (MQ=255)

                                                                        cacgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTgcgc                                                                     >  2:361636/1‑151 (MQ=255)

                                                                         acgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTgcgc                                                                     >  1:166732/1‑150 (MQ=255)

                                                                          cgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCAt                                                                   <  1:263728/151‑1 (MQ=255)

                                                                                     gCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCcac                                                        >  1:509324/1‑151 (MQ=255)

                                                                                         aaCGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCacac                                                      <  1:623641/149‑1 (MQ=255)

                                                                                          aCGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCGCCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCgg                                                   >  1:602532/1‑151 (MQ=255)

                                                                                          aCGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCgg                                                   <  2:28124/151‑1 (MQ=255)

                                                                                                cGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGcc                                             <  1:454280/151‑1 (MQ=255)

                                                                                                  gAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCa                                            >  1:559473/1‑150 (MQ=255)

                                                                                                            tCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCggg                                 >  1:352627/1‑151 (MQ=255)

                                                                                                             cACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCggg                                 <  2:536755/150‑1 (MQ=255)

                                                                                                                gCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCaaa                             <  2:506097/151‑1 (MQ=255)

                                                                                                                          cGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGa                    <  2:419452/150‑1 (MQ=255)

                                                                                                                            cATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGAttt                 >  2:302932/1‑151 (MQ=255)

                                                                                                                              ttAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTc               >  1:76448/1‑151 (MQ=255)

                                                                                                                                     cAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg        <  2:306525/151‑1 (MQ=255)

                                                                                                                                        aaTATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg        >  1:453540/1‑148 (MQ=255)

                                                                                                                                        aaTATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg        >  1:462910/1‑148 (MQ=255)

                                                                                                                                          tatGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg        >  1:181364/1‑146 (MQ=255)

                                                                                                                                           atGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGatcg  <  2:446526/151‑1 (MQ=255)

                                                                                                                                            tGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg        >  1:201928/1‑144 (MQ=255)

CCTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATATTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGATCG  >  NC_000913/2978581‑2978870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A