Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,018,435	A→G	E792E (GAA→GAG)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,018,435	0	A	G	100.0%	146.4 / NA	39	E792E (GAA→GAG)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/18); total (21/18)

GATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACC  >  NC_000913/3018289‑3018576

gATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGccc                                                                                                                                           >  1:70554/1‑151 (MQ=255)

         ggCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCg                                                                                                                                   <  1:50369/150‑1 (MQ=255)

          gCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGAt                                                                                                                                 <  2:58240/151‑1 (MQ=255)

                  aCCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGc                                                                                                                         >  2:80788/1‑151 (MQ=255)

                              aTGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACaaaa                                                                                                             <  2:486552/151‑1 (MQ=255)

                                  cGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACgg                                                                                                         >  1:51015/1‑151 (MQ=255)

                                      tGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTg                                                                                                      <  2:415170/150‑1 (MQ=255)

                                        gCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGcc                                                                                                   <  1:620064/151‑1 (MQ=255)

                                           aaCCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGcc                                                                                                   >  1:123163/1‑148 (MQ=255)

                                              cGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGt                                                                                             <  2:70554/151‑1 (MQ=255)

                                               ggATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTc                                                                                            <  2:55593/151‑1 (MQ=255)

                                                  tGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGAc                                                                                         >  2:610902/1‑151 (MQ=255)

                                                   gagaAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGAc                                                                                         >  2:265764/1‑150 (MQ=255)

                                                      aaaGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATa                                                                                     <  1:332889/151‑1 (MQ=255)

                                                        aGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAAt                                                                                   >  1:417741/1‑151 (MQ=255)

                                                               cgtcgtcCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGaa                                                                             <  1:166644/150‑1 (MQ=255)

                                                                   gtcCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCg                                                                        <  2:315418/151‑1 (MQ=255)

                                                                    tcCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCGTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGt                                                                       >  2:356576/1‑151 (MQ=255)

                                                                     ccGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTc                                                                      >  2:534146/1‑151 (MQ=255)

                                                                      cGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGAGAAACTCGTc                                                                      >  2:444144/1‑150 (MQ=255)

                                                                       ggTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTctg                                                                    >  1:328675/1‑151 (MQ=255)

                                                                       ggTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTctg                                                                    >  1:195524/1‑151 (MQ=255)

                                                                            aaaCCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTctgactg                                                                >  2:541690/1‑150 (MQ=255)

                                                                               ccAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACg                                                             <  2:185993/150‑1 (MQ=255)

                                                                                     aaaCAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTAt                                                      <  2:394619/151‑1 (MQ=255)

                                                                                          gTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCg                                                 <  1:224399/151‑1 (MQ=255)

                                                                                           tAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCg                                                 >  2:179333/1‑150 (MQ=255)

                                                                                              cacTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCg                                              >  1:415537/1‑150 (MQ=255)

                                                                                               acTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGAc                                            >  1:145866/1‑151 (MQ=255)

                                                                                                    ttGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTAc                                        >  2:96145/1‑150 (MQ=255)

                                                                                                      gTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAgc                                     <  1:594854/151‑1 (MQ=255)

                                                                                                      gTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAg                                      <  1:10614/150‑1 (MQ=255)

                                                                                                                        gCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTg                   >  2:578538/1‑151 (MQ=255)

                                                                                                                         ccATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGt                  <  1:270863/151‑1 (MQ=255)

                                                                                                                              ggTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCgctg             <  1:96145/151‑1 (MQ=255)

                                                                                                                                tGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCgctgct           >  2:214523/1‑151 (MQ=255)

                                                                                                                                tGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCgctgct           >  1:359255/1‑151 (MQ=255)

                                                                                                                                    cagcagGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCg       <  1:308569/151‑1 (MQ=255)

                                                                                                                                         ggATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAAcc  >  1:102309/1‑151 (MQ=255)

GATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACC  >  NC_000913/3018289‑3018576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A