Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,398	T→C	V372A (GTA→GCA)	galP →	D‑galactose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,398	0	T	C	100.0%	134.5 / NA	35	V372A (GTA→GCA)	galP	D‑galactose transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/17); total (18/17)

CCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGT  >  NC_000913/3089255‑3089544

ccTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACtgtg                                                                                                                                              <  2:429805/150‑1 (MQ=255)

                ggCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGc                                                                                                                             <  1:196672/151‑1 (MQ=255)

                   aTGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTg                                                                                                                           <  2:436609/150‑1 (MQ=255)

                         gTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGcc                                                                                                                    >  2:105690/1‑151 (MQ=255)

                               ggTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGAtt                                                                                                              <  2:144290/151‑1 (MQ=255)

                                  aCAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTg                                                                                                           >  2:585954/1‑151 (MQ=255)

                                  aCAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTg                                                                                                           <  1:263625/151‑1 (MQ=255)

                                       gacgCATATCGGTATTCCCTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCa                                                                                                        <  2:73253/146‑1 (MQ=255)

                                                cGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTc                                                                                              <  1:529262/150‑1 (MQ=255)

                                                          tctcCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGccacc                                                                                    >  1:580539/1‑150 (MQ=255)

                                                             ccGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACt                                                                                <  1:69125/151‑1 (MQ=255)

                                                              cGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTg                                                                               >  2:522548/1‑151 (MQ=255)

                                                                    cgcAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGc                                                                         >  1:198270/1‑151 (MQ=255)

                                                                      cAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCa                                                                       <  1:462424/151‑1 (MQ=255)

                                                                       aGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCa                                                                       >  2:445633/1‑150 (MQ=255)

                                                                          aTTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACa                                                                    <  1:413340/150‑1 (MQ=255)

                                                                                   tcgccatGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTgg                                                          >  1:354269/1‑151 (MQ=255)

                                                                                    cgccatGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTgg                                                          <  2:256393/150‑1 (MQ=255)

                                                                                             gctgATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTc                                                >  1:398569/1‑151 (MQ=255)

                                                                                                gATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTg                                             >  2:622458/1‑151 (MQ=255)

                                                                                                 aTGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTg                                             >  2:567256/1‑150 (MQ=255)

                                                                                                 aTGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTg                                             >  1:217694/1‑150 (MQ=255)

                                                                                                        ttGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGc                                      >  2:276267/1‑150 (MQ=255)

                                                                                                         tGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTc                                    >  1:238843/1‑151 (MQ=255)

                                                                                                          gTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTc                                    >  2:65341/1‑150 (MQ=255)

                                                                                                          gTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCAAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCa                                   <  2:435839/151‑1 (MQ=255)

                                                                                                                tttGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGc                             >  1:372593/1‑151 (MQ=255)

                                                                                                                 ttGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGc                             <  1:137896/150‑1 (MQ=255)

                                                                                                                         aGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACg                    <  2:390892/151‑1 (MQ=255)

                                                                                                                              cGGTCCGCTGATTTGGGCACTGTGCTCCGAACTTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAac               >  1:238925/1‑151 (MQ=255)

                                                                                                                                gTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCGCCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAacac             <  2:10010/151‑1 (MQ=255)

                                                                                                                                    gCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTc         >  2:560998/1‑151 (MQ=255)

                                                                                                                                       gATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCt        <  1:218729/149‑1 (MQ=255)

                                                                                                                                           tGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGgtgt  >  2:412411/1‑151 (MQ=255)

                                                                                                                                           tGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGgtg   <  1:257850/150‑1 (MQ=255)

CCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGT  >  NC_000913/3089255‑3089544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A