Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,376,403	T→C	D125G (GAC→GGC)	sspB ←	ClpXP protease specificity enhancing factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,376,403	0	T	C	100.0%	115.1 / NA	33	D125G (GAC→GGC)	sspB	ClpXP protease specificity enhancing factor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/15); total (18/15)

CAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGACGCGGAATGCCACC  >  NC_000913/3376255‑3376548

cAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCt                                                                                                                                                 >  2:582096/1‑151 (MQ=255)

     cgccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGa                                                                                                                                            <  1:105887/151‑1 (MQ=255)

           ggCCTGTTTTGTATTACTTCACACCGCGTAAGGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTAATCGTCAGGATGGGTGTCATCATCGTGACCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCctc                                                                                                                                      <  2:627623/151‑1 (MQ=255)

            gCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCctct                                                                                                                                     >  1:164210/1‑151 (MQ=255)

                      tATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCAtcattc                                                                                                                            <  2:515000/150‑1 (MQ=255)

                          aCTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCatgat                                                                                                                       >  2:396001/1‑151 (MQ=255)

                                   cgcgTAATGCCGGTCGACCGCCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTAt                                                                                                               <  2:7291/150‑1 (MQ=255)

                                    gcgTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCt                                                                                                             >  1:489386/1‑151 (MQ=255)

                                     cgTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCtt                                                                                                            <  1:143725/151‑1 (MQ=255)

                                         aTGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATc                                                                                                        <  1:406578/151‑1 (MQ=255)

                                             cGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAg                                                                                                    <  1:609982/151‑1 (MQ=255)

                                                      accGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTc                                                                                            >  1:610592/1‑150 (MQ=255)

                                                           gtggtgGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTc                                                                                      <  2:26370/151‑1 (MQ=255)

                                                              gtggCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCaa                                                                                    >  1:147844/1‑150 (MQ=255)

                                                                                   cAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTc                                                              >  2:105343/1‑151 (MQ=255)

                                                                                       aTGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACgg                                                          >  2:341663/1‑151 (MQ=255)

                                                                                         gAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGc                                                        <  2:507662/151‑1 (MQ=255)

                                                                                               catcatCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGa                                                   <  1:556163/150‑1 (MQ=255)

                                                                                               catcatCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGa                                                   <  1:582111/150‑1 (MQ=255)

                                                                                                    tcGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGcc                                              >  1:234584/1‑150 (MQ=255)

                                                                                                               cTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGcagcc                                  >  2:219627/1‑151 (MQ=255)

                                                                                                                       cATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGcac                          <  2:325861/151‑1 (MQ=255)

                                                                                                                           aaTAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGaa                      <  2:553473/151‑1 (MQ=255)

                                                                                                                           aaTAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGa                       >  1:400264/1‑150 (MQ=255)

                                                                                                                            aTAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGaaa                     <  1:428347/151‑1 (MQ=255)

                                                                                                                            aTAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGaa                      >  1:499917/1‑150 (MQ=255)

                                                                                                                                ccGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTg                 >  2:141402/1‑151 (MQ=255)

                                                                                                                                ccGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTg                 >  2:174316/1‑151 (MQ=255)

                                                                                                                                ccGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTg                 >  1:249641/1‑151 (MQ=255)

                                                                                                                                  gACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGAc               <  2:36079/151‑1 (MQ=255)

                                                                                                                                   aCATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGAcg              >  1:291948/1‑151 (MQ=255)

                                                                                                                                       aaCGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGACGCgg           >  2:97383/1‑150 (MQ=255)

                                                                                                                                                gTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGACGCGGAATGccacc  >  2:263252/1‑150 (MQ=255)

CAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGACGCGGAATGCCACC  >  NC_000913/3376255‑3376548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A