Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,436,036	A→G	D277G (GAT→GGT)	rsmB →	16S rRNA m(5)C967 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,436,036	0	A	G	100.0%	153.8 / NA	43	D277G (GAT→GGT)	rsmB	16S rRNA m(5)C967 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/24); total (19/24)

ACCGTTCAGGATGCATCAGCACAAGGTTGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCT  >  NC_000913/3435891‑3436178

aCCGTTCAGGATGCATCAGCACAAGGTTGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATcg                                                                                                                                           <  2:622739/151‑1 (MQ=255)

   gTTCAGGATGCATCAGCACAAGGTTGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATcgacg                                                                                                                                        >  1:168144/1‑151 (MQ=255)

          aTGCATCAGCACAAGGTTGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAgcg                                                                                                                                 >  1:42355/1‑151 (MQ=255)

              aTCAGCACAAGGTTGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCctc                                                                                                                             <  1:446491/151‑1 (MQ=255)

                    acaAGGTTGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCgg                                                                                                                       <  2:147707/151‑1 (MQ=255)

                    acaAGGTTGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCgg                                                                                                                       <  1:515433/151‑1 (MQ=255)

                            gCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACg                                                                                                                <  2:553770/150‑1 (MQ=255)

                            gCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACg                                                                                                                >  2:172726/1‑150 (MQ=255)

                               tGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACa                                                                                                             >  1:623983/1‑150 (MQ=255)

                                    tGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTa                                                                                                        >  1:244519/1‑150 (MQ=255)

                                     ggCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTaaa                                                                                                      <  1:300804/151‑1 (MQ=255)

                                         tGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACg                                                                                                   >  1:23192/1‑150 (MQ=255)

                                              cacaAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTgg                                                                                             <  1:461367/151‑1 (MQ=255)

                                               acaAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGt                                                                                            <  1:15/151‑1 (MQ=255)

                                                   aaCGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATg                                                                                         >  1:588874/1‑150 (MQ=255)

                                                    aCGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGaa                                                                                       <  1:591669/151‑1 (MQ=255)

                                                       gTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGc                                                                                    <  2:40239/151‑1 (MQ=255)

                                                        tGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGCTTACGACAATTTAAAACGCCTTGGTATGAAGGc                                                                                    >  2:575384/1‑150 (MQ=255)

                                                         gAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGa                                                                                  <  1:170019/151‑1 (MQ=255)

                                                          aaCACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGAc                                                                                 >  1:73989/1‑151 (MQ=255)

                                                              caTTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTg                                                                             <  1:379799/151‑1 (MQ=255)

                                                              caTTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTg                                                                             <  1:464598/151‑1 (MQ=255)

                                                                     gATCTTTGTGCCGCCCCCGGCGGTAAACCAGCGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACa                                                                        >  1:192772/1‑149 (MQ=255)

                                                                          ttGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAgg                                                                     <  2:506892/147‑1 (MQ=255)

                                                                            gtgCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATgg                                                               >  1:306203/1‑151 (MQ=255)

                                                                                         cggTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTtct                                                  >  1:413359/1‑151 (MQ=255)

                                                                                            tAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAATAAGGTGATGGCCGTTACCCTTCTCa                                                >  2:375253/1‑150 (MQ=255)

                                                                                              aaaCAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATg                                             <  1:511929/151‑1 (MQ=255)

                                                                                                 caacGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGgtg                                          >  2:258684/1‑151 (MQ=255)

                                                                                                  aacGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGgtgt                                         >  1:385723/1‑151 (MQ=255)

                                                                                                         atCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAg                                   <  2:581660/150‑1 (MQ=255)

                                                                                                              tGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAAc                               <  1:488629/149‑1 (MQ=255)

                                                                                                              tGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAAc                               >  1:383745/1‑149 (MQ=255)

                                                                                                                             aGCGCAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTtta              <  2:23192/151‑1 (MQ=255)

                                                                                                                              gcgcAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTttat             <  1:66016/151‑1 (MQ=255)

                                                                                                                              gcgcAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTttat             <  1:254341/151‑1 (MQ=255)

                                                                                                                               cgcAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTttatt            <  1:154706/151‑1 (MQ=255)

                                                                                                                                gcAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTttatta           <  2:598827/151‑1 (MQ=255)

                                                                                                                                gcAGGTTGTTGCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTttatta           <  1:171254/151‑1 (MQ=255)

                                                                                                                                 cAGGTTGTTGCGGTTGGTATCGGCGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTttatta           <  1:113356/150‑1 (MQ=255)

                                                                                                                                      tgttgCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATgcg     >  2:496023/1‑151 (MQ=255)

                                                                                                                                       gttgCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATgcgc    >  1:46540/1‑151 (MQ=255)

                                                                                                                                         tgCGGTTGGTATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCt  >  1:149130/1‑151 (MQ=255)

ACCGTTCAGGATGCATCAGCACAAGGTTGCATGACCTGGCTTGCGCCACAAAACGGTGAACACATTTTGGATCTTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCT  >  NC_000913/3435891‑3436178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A