Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,507,100	T→C	L79L (TTA→TTG)	yhfT ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,507,100	0	T	C	100.0%	104.2 / NA	29	L79L (TTA→TTG)	yhfT	inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/12); total (17/12)

CTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATG  >  NC_000913/3506955‑3507244

cTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAAcc                                                                                                                                              >  1:590436/1‑150 (MQ=255)

      cATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGtt                                                                                                                                       <  2:348668/151‑1 (MQ=255)

       aTCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTg                                                                                                                                      <  2:590421/151‑1 (MQ=255)

       aTCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTg                                                                                                                                      >  1:403695/1‑151 (MQ=255)

              ggCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGcc                                                                                                                               >  2:462086/1‑151 (MQ=255)

                             agcTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTa                                                                                                                 >  2:495983/1‑150 (MQ=255)

                                    ttACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAag                                                                                                             >  2:47453/1‑147 (MQ=255)

                                        tGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATcccc                                                                                                     >  2:438248/1‑151 (MQ=255)

                                         ggCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCTAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCa                                                                                                    <  1:542302/151‑1 (MQ=255)

                                         ggCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCa                                                                                                    <  1:619833/151‑1 (MQ=255)

                                                 gCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGcc                                                                                            >  2:80397/1‑151 (MQ=255)

                                                   aaGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCcac                                                                                          >  1:491653/1‑151 (MQ=255)

                                                        aGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAAc                                                                                     >  1:139510/1‑151 (MQ=255)

                                                         ggATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAAcc                                                                                    <  2:82177/151‑1 (MQ=255)

                                                              aaCCCGCCCCAAATAGCGCCAAGACAAAACGCCATCAGGCTG‑TTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATg                                                                                <  1:334731/149‑1 (MQ=255)

                                                               acacGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGc                                                                               <  2:162694/150‑1 (MQ=255)

                                                                               gcCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGAt                                                               >  1:591939/1‑150 (MQ=255)

                                                                                 cAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACt                                                            >  1:115392/1‑151 (MQ=255)

                                                                                     aCCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTcccc                                                        >  2:612399/1‑151 (MQ=255)

                                                                                     aCCAAACGACATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTcccc                                                        <  2:371554/151‑1 (MQ=255)

                                                                                                    gCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTc                                         >  2:139733/1‑151 (MQ=255)

                                                                                                                 aGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCa                             >  1:442193/1‑150 (MQ=255)

                                                                                                                 aGGACGCCGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCa                             >  1:229109/1‑150 (MQ=255)

                                                                                                                       ccGAGGATATCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGt                       <  2:84105/150‑1 (MQ=255)

                                                                                                                             atatCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGc                 >  1:348129/1‑150 (MQ=255)

                                                                                                                               atCGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAg               <  1:566244/150‑1 (MQ=255)

                                                                                                                                 cGGTAGGAAGAAAGAGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGa             <  2:538635/150‑1 (MQ=255)

                                                                                                                                           aaagaGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGAt   <  2:115392/150‑1 (MQ=255)

                                                                                                                                           aaagaGCAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATg  >  2:369062/1‑151 (MQ=255)

CTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATG  >  NC_000913/3506955‑3507244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A