Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,540,736	A→G	E110E (GAA→GAG)	feoB →	ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,540,736	0	A	G	100.0%	137.5 / NA	37	E110E (GAA→GAG)	feoB	ferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/21); total (16/21)

CGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGC  >  NC_000913/3540609‑3540879

cGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTgg                                                                                                                           <  1:314619/150‑1 (MQ=255)

     aCCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGa                                                                                                                     <  2:268789/151‑1 (MQ=255)

         cgctcgATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACa                                                                                                                  <  2:77895/150‑1 (MQ=255)

           ctcgATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGc                                                                                                               >  1:112877/1‑151 (MQ=255)

             cgATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTc                                                                                                             <  1:216719/151‑1 (MQ=255)

                      aaTCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGcc                                                                                                    <  1:603891/151‑1 (MQ=255)

                         cGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCgag                                                                                                 <  1:229249/151‑1 (MQ=255)

                          gCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCg                                                                                                   >  2:211131/1‑148 (MQ=255)

                               tCACTACATTTTGAGTGGCGACGCCGACCTTCTGATTAACGTGGTGGATGCGTCTACCCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGc                                                                                             <  2:223808/149‑1 (MQ=255)

                                 aCTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCaaa                                                                                          >  1:460594/1‑150 (MQ=255)

                                    aCATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAat                                                                                        <  1:226075/149‑1 (MQ=255)

                                     cATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATAtt                                                                                     >  2:378792/1‑151 (MQ=255)

                                       ttttGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCg                                                                                   >  2:512834/1‑151 (MQ=255)

                                        tttGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGt                                                                                  >  2:380325/1‑151 (MQ=255)

                                           gAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTAt                                                                                <  1:23286/150‑1 (MQ=255)

                                           gAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTAt                                                                                <  1:221321/150‑1 (MQ=255)

                                             gTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTg                                                                              >  1:109323/1‑150 (MQ=255)

                                               ggCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGaa                                                                            <  1:555361/150‑1 (MQ=255)

                                               ggCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGaa                                                                            <  2:611478/150‑1 (MQ=255)

                                               ggCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGaa                                                                            <  2:184108/150‑1 (MQ=255)

                                               ggCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGaa                                                                            <  1:277065/150‑1 (MQ=255)

                                                      ccGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGc                                                                    <  1:519450/151‑1 (MQ=255)

                                                            tgctgATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTc                                                              >  2:78963/1‑151 (MQ=255)

                                                             gctgATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTc                                                              <  1:380332/150‑1 (MQ=255)

                                                                       gtggtgGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTgg                                                   >  2:231212/1‑151 (MQ=255)

                                                                         ggtggATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGc                                                 >  2:339119/1‑151 (MQ=255)

                                                                          gtggATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCt                                                <  1:23568/151‑1 (MQ=255)

                                                                            ggATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTg                                               <  1:3708/150‑1 (MQ=255)

                                                                                   tCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTg                                        >  2:93616/1‑150 (MQ=255)

                                                                                      aaCCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTTGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATcc                                    >  1:598073/1‑151 (MQ=255)

                                                                                      aaCCTGGAGCGTAGCCTGTACCTGACGGTACAGCGGCTGGAGCTCGGCATGCCCTGCATTGTGGCACTGAACATGCTCGACAGTGCCGAGAAGCAAAATATGCGTATTGAAAGTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGa                                       <  2:329555/148‑1 (MQ=255)

                                                                                       aCCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATccc                                   >  2:504758/1‑151 (MQ=255)

                                                                                                gTAACCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTc                          >  1:583799/1‑151 (MQ=255)

                                                                                                   aCCTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAAc                       >  2:70442/1‑151 (MQ=255)

                                                                                                    ccTGTACCTGACGCTACAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAAcc                      >  2:419286/1‑151 (MQ=255)

                                                                                                                    cAACTGCTGGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTAtt       <  1:419293/150‑1 (MQ=255)

                                                                                                                         gctgGAGCTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGaatc  <  1:483826/150‑3 (MQ=255)

CGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGC  >  NC_000913/3540609‑3540879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A