Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,844,470	A→G	E169E (GAA→GAG)	adeD →	cryptic adenine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,844,470	0	A	G	100.0%	126.6 / NA	34	E169E (GAA→GAG)	adeD	cryptic adenine deaminase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/20); total (14/20)

CAACGTGATGGGCGAAGCCGGATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCAC  >  NC_000913/3844332‑3844605

cAACGTGATGGGCGAAGCCGGATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGcc                                                                                                                             >  1:195888/1‑151 (MQ=255)

 aaCGTGATGGGCGAAGCCGGATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCt                                                                                                                            <  2:541355/151‑1 (MQ=255)

   cGTGATGGGCGAAGCCGGATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTgg                                                                                                                          <  1:143927/151‑1 (MQ=255)

   cGTGATGGGCGAAGCCGGATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTgg                                                                                                                          >  1:554066/1‑151 (MQ=255)

           gCGAAGCCGGATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCa                                                                                                                  <  1:469269/151‑1 (MQ=255)

                  cGGATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGc                                                                                                             >  1:225185/1‑149 (MQ=255)

                   ggATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGGGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGc                                                                                                             >  2:391841/1‑148 (MQ=255)

                     aTTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGtt                                                                                                        >  2:77585/1‑151 (MQ=255)

                      ttCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGtt                                                                                                        >  1:330179/1‑150 (MQ=255)

                      ttCGCCTGGTTTGCCCGCTGTGCCGAACAGGAAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGtt                                                                                                        <  2:502102/150‑1 (MQ=255)

                        cGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTAcc                                                                                                     <  1:107380/151‑1 (MQ=255)

                              gTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCtt                                                                                               >  1:300958/1‑151 (MQ=255)

                                ttGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTg                                                                                              <  1:30982/150‑1 (MQ=255)

                                     cGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGaaa                                                                                        <  2:534928/151‑1 (MQ=255)

                                         gtgCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAatga                                                                                     <  1:244372/150‑1 (MQ=255)

                                          tgCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAatgatg                                                                                   >  1:405787/1‑151 (MQ=255)

                                           gCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATgg                                                                                  <  2:37771/151‑1 (MQ=255)

                                             cGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGAc                                                                                >  1:116877/1‑151 (MQ=255)

                                                aCAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTAc                                                                             <  2:625412/151‑1 (MQ=255)

                                                   ggcaaggcaaAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCt                                                                          <  1:443061/151‑1 (MQ=255)

                                                          caaAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTa                                                                    >  1:87830/1‑150 (MQ=255)

                                                                 aGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGGTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTATCgg                                                            <  2:165494/151‑1 (MQ=255)

                                                                    aCTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGc                                                          >  1:478357/1‑150 (MQ=255)

                                                                         cAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATg                                                    <  2:347721/151‑1 (MQ=255)

                                                                              cAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCgct                                                <  1:214793/150‑1 (MQ=255)

                                                                               aGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACTATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCgctgc                                              <  2:39164/151‑1 (MQ=255)

                                                                                    ttGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGAt                                         >  1:323328/1‑151 (MQ=255)

                                                                                             cGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGAt                                <  1:162620/151‑1 (MQ=255)

                                                                                              gCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATg                               >  2:399333/1‑151 (MQ=255)

                                                                                                   ggAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCAttt                          >  1:124909/1‑151 (MQ=255)

                                                                                                           gCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCAcc                   <  1:384253/150‑1 (MQ=255)

                                                                                                           gCGATGTTAACGGTGCCAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCAc                    <  1:281245/149‑1 (MQ=255)

                                                                                                                           cAGTTTTACCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCAc  <  1:83435/151‑1 (MQ=255)

                                                                                                                            aGTTTTGCCCTTGAGCAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCAc  <  1:344991/150‑1 (MQ=255)

CAACGTGATGGGCGAAGCCGGATTCGCCTGGTTTGCCCGCTGTGCCGAACAGGCAAGGCAAAACCAGTACTTACAGGTCAGCTCTTGCGTACCCGCCCTGGAAGGCTGCGATGTTAACGGTGCCAGTTTTACCCTTGAACAGATGCTCGCCTGGCGGGACCATCCGCAGGTTACCGGCCTTGCAGAAATGATGGACTACCCTGGCGTAATTAGCGGGCAGAATGCGCTGCTCGATAAACTGGATGCATTTCGCCACCTGACGCTGGACGGTCAC  >  NC_000913/3844332‑3844605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A