Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,005,381	A→G	S174G (AGC→GGC)	pldA →	outer membrane phospholipase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,005,381	0	A	G	100.0%	119.3 / NA	34	S174G (AGC→GGC)	pldA	outer membrane phospholipase A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/21); total (13/21)

CTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATC  >  NC_000913/4005238‑4005525

cTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGa                                                                                                                                            >  2:362939/1‑150 (MQ=255)

 ttCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGaa                                                                                                                                           >  2:223313/1‑150 (MQ=255)

   cACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCg                                                                                                                                        <  2:586068/151‑1 (MQ=255)

           cGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCCCCGATTCCCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATACCCACGCCTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTtat                                                                                                                                 <  2:592536/150‑1 (MQ=255)

           cGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTtat                                                                                                                                 >  1:404717/1‑150 (MQ=255)

                  ccAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCt                                                                                                                         >  1:256070/1‑151 (MQ=255)

                                  aTTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGt                                                                                                         >  2:226632/1‑151 (MQ=255)

                                      ttCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGCCGCTGCGCGATGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTCCCGACCCGACCTCCCGCGCCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTaa                                                                                                       <  2:305389/149‑1 (MQ=255)

                                        ccTCGGTTTTGCCACCGATTACCGTTTTGCGGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAAct                                                                                                     <  1:102663/149‑1 (MQ=255)

                                               tttGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGt                                                                                              >  1:594276/1‑149 (MQ=255)

                                               tttGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTa                                                                                             <  1:443334/150‑1 (MQ=255)

                                               tttGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTa                                                                                             <  1:347995/150‑1 (MQ=255)

                                                  gCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAg                                                                                         <  2:578298/151‑1 (MQ=255)

                                                        gATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGc                                                                                   >  2:358928/1‑151 (MQ=255)

                                                               gTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGTCGTTCCGACCCGACTTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTa                                                                            <  2:200557/151‑1 (MQ=255)

                                                                      aGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATgtggtg                                                                     >  1:199693/1‑151 (MQ=255)

                                                                      aGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATgtggtg                                                                     >  1:18139/1‑151 (MQ=255)

                                                                             aCGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAAt                                                               >  1:440043/1‑150 (MQ=255)

                                                                               ggtGCGCGATGTGGAGATGGGGTATACCCACGACTCTAACGGGCGTTCCGACCCGCCCCCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACt                                                            <  1:498631/149‑1 (MQ=255)

                                                                               gCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACt                                                            >  2:599961/1‑151 (MQ=255)

                                                                                     cgATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGAt                                                      <  1:131261/151‑1 (MQ=255)

                                                                                        tgtgGAGATGGGGTATAACCACGATTTTAACGGGCGTTCCGACCGGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATaa                                                    <  1:53718/150‑1 (MQ=255)

                                                                                                  gggTATAACCACGACTCTAACGGGCGTCCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCa                                         <  2:440035/151‑1 (MQ=255)

                                                                                                     tataACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCAcc                                       <  1:385081/150‑1 (MQ=255)

                                                                                                        aaCCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAat                                   >  2:130558/1‑151 (MQ=255)

                                                                                                                    aaCGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTta                         <  2:219950/149‑1 (MQ=255)

                                                                                                                         gCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAg                  <  2:124789/151‑1 (MQ=255)

                                                                                                                          cGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAg                  <  2:275688/150‑1 (MQ=255)

                                                                                                                              ccgacccgacCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCtt               <  2:184624/149‑1 (MQ=255)

                                                                                                                              ccgacccgacCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTa              <  2:3181/150‑1 (MQ=255)

                                                                                                                               cgacccgaccTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTaaa            <  1:318647/151‑1 (MQ=255)

                                                                                                                                gacccgaccTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCtt               <  2:57928/147‑1 (MQ=255)

                                                                                                                                  cccgaccTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAAt          <  2:288263/150‑1 (MQ=255)

                                                                                                                                         tCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATc  >  2:263262/1‑151 (MQ=255)

CTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATC  >  NC_000913/4005238‑4005525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A