Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,229,213	C→T	L495L (CTG→TTG)	yjbB →	putative Na+/Pi‑cotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,229,213	0	C	T	100.0%	162.0 / NA	42	L495L (CTG→TTG)	yjbB	putative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/21); total (21/21)

TCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCCTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACG  >  NC_000913/4229070‑4229354

tctATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATc                                                                                                                                         >  2:361264/1‑150 (MQ=255)

     gAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCaa                                                                                                                                    <  1:361271/150‑1 (MQ=255)

             gCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCa                                                                                                                            <  1:207957/150‑1 (MQ=255)

                 aGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGc                                                                                                                        >  1:114595/1‑150 (MQ=255)

                      tttAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGa                                                                                                                   >  2:100437/1‑150 (MQ=255)

                         aaaGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCTCGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGaaa                                                                                                                 <  1:159019/149‑1 (MQ=255)

                         aaaGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAAc                                                                                                                <  1:329652/150‑1 (MQ=255)

                          aaGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAAcc                                                                                                               >  2:163490/1‑150 (MQ=255)

                             cTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGtt                                                                                                           >  1:281429/1‑151 (MQ=255)

                               ggCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGtt                                                                                                           <  2:187783/149‑1 (MQ=255)

                                gCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGc                                                                                                        <  2:12641/151‑1 (MQ=255)

                                   aTGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTAc                                                                                                     <  2:213650/151‑1 (MQ=255)

                                     gTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACAt                                                                                                   <  2:176321/151‑1 (MQ=255)

                                           gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAgg                                                                                              <  1:398306/150‑1 (MQ=255)

                                           gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGc                                                                                             >  1:215756/1‑151 (MQ=255)

                                                     ggCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTgg                                                                                      <  1:18960/148‑1 (MQ=255)

                                                         aTGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGatat                                                                               <  2:628386/151‑1 (MQ=255)

                                                             cACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGc                                                                             >  1:582608/1‑149 (MQ=255)

                                                             cACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGc                                                                             >  1:381490/1‑149 (MQ=255)

                                                                    gcTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGa                                                                    <  2:412352/151‑1 (MQ=255)

                                                                           gtTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCt                                                             <  2:413761/151‑1 (MQ=255)

                                                                             ttGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCtt                                                            <  2:41272/150‑2 (MQ=255)

                                                                                cGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGtttt                                                        >  2:182363/1‑151 (MQ=255)

                                                                                   cGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGtt                                                     >  1:381752/1‑151 (MQ=255)

                                                                                   cGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGtt                                                     >  2:260453/1‑151 (MQ=255)

                                                                                           aCATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGc                                              <  2:559006/150‑1 (MQ=255)

                                                                                              tCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTAc                                          >  1:339752/1‑151 (MQ=255)

                                                                                              tCGTTTTCGCATTCTTAATCGCCGCTATCCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTAc                                          >  1:116989/1‑151 (MQ=255)

                                                                                                 ttttCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAgt                                       >  1:129298/1‑151 (MQ=255)

                                                                                                       cATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTg                                 >  1:376683/1‑151 (MQ=255)

                                                                                                          tCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGa                               <  2:150562/150‑1 (MQ=255)

                                                                                                                tCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCg                        >  2:632891/1‑151 (MQ=255)

                                                                                                                   ccgcTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgat                     <  2:509947/151‑1 (MQ=255)

                                                                                                                   ccgcTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgat                     >  2:119698/1‑151 (MQ=255)

                                                                                                                           cccacgcccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgatgaagatg              <  2:582593/150‑1 (MQ=255)

                                                                                                                           cccacgcccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgatga                   >  1:170072/1‑145 (MQ=255)

                                                                                                                            ccacgcccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgatgaagatgac            <  2:503918/151‑2 (MQ=255)

                                                                                                                             cacgcccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgatgaagatga             >  2:82687/1‑149 (MQ=255)

                                                                                                                              acgcccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAgg          <  1:565964/151‑1 (MQ=255)

                                                                                                                                 cccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAggacg       >  2:192812/1‑151 (MQ=255)

                                                                                                                                  ccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAggacgg      >  1:529511/1‑151 (MQ=255)

                                                                                                                                      gTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAggacgggacg  <  1:397238/151‑1 (MQ=255)

TCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCCTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACG  >  NC_000913/4229070‑4229354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A