Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,631,960	C→T	P410S (CCG→TCG)	slt →	lytic murein transglycosylase, soluble

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,631,960	0	C	T	100.0%	154.6 / NA	39	P410S (CCG→TCG)	slt	lytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/16); total (23/16)

GAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAAT  >  NC_000913/4631816‑4632095

gaagctgaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGgaga                                                                                                                                   <  2:26136/151‑1 (MQ=255)

   gctgaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATgg                                                                                                                                >  2:144288/1‑151 (MQ=255)

           aaaaGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTg                                                                                                                        >  2:614225/1‑151 (MQ=255)

               gagaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGcgcg                                                                                                                    >  1:244582/1‑151 (MQ=255)

                agaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGcgcg                                                                                                                    >  1:380525/1‑150 (MQ=255)

                 gaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGcgcg                                                                                                                    >  2:2964/1‑149 (MQ=255)

                    tttGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTg                                                                                                               >  1:619681/1‑151 (MQ=255)

                                   gCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAAtctc                                                                                                <  1:235504/151‑1 (MQ=255)

                                        aGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGata                                                                                            >  2:134428/1‑150 (MQ=255)

                                           gTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATAc                                                                                        <  2:173603/151‑1 (MQ=255)

                                             ggTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcg                                                                                      >  1:473281/1‑151 (MQ=255)

                                                ttCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgcgc                                                                                   >  2:467775/1‑151 (MQ=255)

                                                ttCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgcgc                                                                                   >  2:385210/1‑151 (MQ=255)

                                                ttCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgcgc                                                                                   >  2:76535/1‑151 (MQ=255)

                                                   tACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGt                                                                                 >  1:376285/1‑150 (MQ=255)

                                                      ccGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCg                                                                             <  2:498898/151‑1 (MQ=255)

                                                        gATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAg                                                                           <  1:611517/151‑1 (MQ=255)

                                                           ggTTGCAGCACAACGCATCGGCGAAGAGTAGGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTg                                                                         <  2:348053/150‑1 (MQ=255)

                                                                   cacaACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCt                                                                <  2:376278/151‑1 (MQ=255)

                                                                      aaCGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGTCAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGGGTGCGCGAGTTGATGTACTGGAATCTCGATAATGCCGCGCGTAGCGAGTGGGCCCATCTGGt                                                             <  2:228056/151‑1 (MQ=255)

                                                                       aCGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTg                                                            >  1:527172/1‑151 (MQ=255)

                                                                       aCGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTg                                                            <  1:109443/151‑1 (MQ=255)

                                                                                cGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAg                                                   <  1:137060/151‑1 (MQ=255)

                                                                                      gTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCaaa                                              >  2:137159/1‑150 (MQ=255)

                                                                                        aTGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAAc                                           >  1:609340/1‑151 (MQ=255)

                                                                                          gAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACag                                         <  1:449887/151‑1 (MQ=255)

                                                                                             cTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCGAGTCAAAAACAGAGc                                      >  2:218021/1‑151 (MQ=255)

                                                                                             cTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGc                                      >  2:85203/1‑151 (MQ=255)

                                                                                              tGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGc                                      >  1:592212/1‑150 (MQ=255)

                                                                                                aaGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAgg                                   <  1:132665/151‑1 (MQ=255)

                                                                                                 aGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGc                                  >  1:583367/1‑151 (MQ=255)

                                                                                                     tGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCa                               >  2:17971/1‑150 (MQ=255)

                                                                                                     tGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCa                               <  2:609593/150‑1 (MQ=255)

                                                                                                          aaGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGgc                         >  2:625179/1‑151 (MQ=255)

                                                                                                           aGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGgcg                        <  2:468832/151‑1 (MQ=255)

                                                                                                                cgcAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGt                    >  2:212920/1‑150 (MQ=255)

                                                                                                                     aaTGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCtt              >  1:83708/1‑151 (MQ=255)

                                                                                                                                cgcCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCaa   <  2:256694/151‑1 (MQ=255)

                                                                                                                                 gcCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAAt  <  1:371038/151‑1 (MQ=255)

GAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAAT  >  NC_000913/4631816‑4632095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A