Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I2 R1	121	82.0	2593086	97.7%	2533445	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,651,365	T→C	Q308R (CAA→CGA)	yfhM ←	bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,651,365	0	T	C	98.7%	272.2 / ‑4.2	75	Q308R (CAA→CGA)	yfhM	bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (35/39); total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

TTACGCGCCAACAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTTGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCCTGCTGAAGCGGTT  >  NC_000913/2651218‑2651509

ttACGCGCCAACAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCgg                                                                                                                                                <  2:1153699/150‑1 (MQ=255)

  aCGCGCCAACAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGt                                                                                                                                              <  2:49959/150‑1 (MQ=255)

    gcgcCAACAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTaaa                                                                                                                                           <  1:383413/151‑1 (MQ=255)

     cgcCAACAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAg                                                                                                                                          <  2:282470/151‑1 (MQ=255)

      gcCAACAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTTGGGTAAAGa                                                                                                                                         <  2:7574/151‑1 (MQ=255)

      gcCAACAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTaa                                                                                                                                            >  2:766106/1‑148 (MQ=255)

        cAACAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGat                                                                                                                                        >  2:845960/1‑150 (MQ=255)

        cAACAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGat                                                                                                                                        >  2:943747/1‑150 (MQ=255)

         aaCAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGatat                                                                                                                                      <  1:1128654/151‑1 (MQ=255)

          aCAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATc                                                                                                                                     <  2:1098663/151‑1 (MQ=255)

          aCAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATc                                                                                                                                     >  1:1094749/1‑151 (MQ=255)

            aGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATcc                                                                                                                                    >  2:285109/1‑150 (MQ=255)

               aaTGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGa                                                                                                                                 <  2:1037592/150‑1 (MQ=255)

                       gTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGa                                                                                                                        <  1:379618/151‑1 (MQ=255)

                          tttATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATa                                                                                                                      <  2:871422/150‑1 (MQ=255)

                            tATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACg                                                                                                                   >  2:20687/1‑151 (MQ=255)

                             aTCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACgg                                                                                                                  >  2:431936/1‑151 (MQ=255)

                               cATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTg                                                                                                                >  1:28741/1‑151 (MQ=255)

                                  tttCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGc                                                                                                             >  2:1241311/1‑151 (MQ=255)

                                   ttCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCt                                                                                                            >  2:326097/1‑151 (MQ=255)

                                       aGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGaa                                                                                                         >  1:1104438/1‑150 (MQ=255)

                                        gCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAAc                                                                                                       <  1:600192/151‑1 (MQ=255)

                                         cTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACg                                                                                                      >  1:499732/1‑151 (MQ=255)

                                             acaTGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCg                                                                                                   >  1:1236839/1‑150 (MQ=255)

                                                    ccTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACt                                                                                           <  2:60573/151‑1 (MQ=255)

                                                       gAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACtt                                                                                          <  1:845922/149‑1 (MQ=255)

                                                       gAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTa                                                                                         <  2:827065/150‑1 (MQ=255)

                                                       gAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTa                                                                                         <  2:425584/150‑1 (MQ=255)

                                                       gAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTa                                                                                         >  2:1141257/1‑150 (MQ=255)

                                                            tCACTGGTTGCCTGAGTCAGAGTCTGCTCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTa                                                                                    <  2:785387/150‑1 (MQ=255)

                                                             cACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTaa                                                                                   <  2:644092/150‑1 (MQ=255)

                                                                 ggTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTACGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACa                                                                                <  1:766070/149‑1 (MQ=255)

                                                                  gTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCg                                                                             <  2:388281/151‑1 (MQ=255)

                                                                     gCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTcg                                                                          <  2:1145006/151‑1 (MQ=255)

                                                                         gAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCggg                                                                      <  1:374008/151‑1 (MQ=255)

                                                                          aGTCAGAGTCTGCCCTTTCTCATTTAATAACGAGACTTCAATTCCTTGCTGGGACGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCggg                                                                      >  1:340460/1‑150 (MQ=255)

                                                                             cAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATAGCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGAtt                                                                   <  1:662665/150‑1 (MQ=255)

                                                                               gagTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACt                                                                >  2:544804/1‑151 (MQ=255)

                                                                                     gCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAAt                                                           <  1:719216/150‑1 (MQ=255)

                                                                                     gCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATc                                                          <  1:105672/151‑1 (MQ=255)

                                                                                       ccTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGt                                                        <  1:1166328/151‑1 (MQ=255)

                                                                                          ttCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTaa                                                      <  2:1122934/150‑1 (MQ=255)

                                                                                              cATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGt                                                   <  2:905183/149‑1 (MQ=255)

                                                                                              cATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGt                                                   <  1:824145/149‑1 (MQ=255)

                                                                                              cATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTcc                                                 >  2:1022844/1‑151 (MQ=255)

                                                                                              cATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTc                                                  <  2:758360/150‑1 (MQ=255)

                                                                                                tttAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTcc                                                 >  2:275567/1‑149 (MQ=255)

                                                                                                tttAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTcc                                                 >  2:64221/1‑149 (MQ=255)

                                                                                                  taataaAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTCACGTcc                                                 >  1:980532/1‑147 (MQ=255)

                                                                                                   aataaAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGcc                                             <  1:273675/150‑1 (MQ=255)

                                                                                                    ataaAGCGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTg                                           >  1:912058/1‑151 (MQ=255)

                                                                                                        agagACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATtc                                       >  2:951468/1‑151 (MQ=255)

                                                                                                          agaCTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATtcat                                     >  2:395446/1‑151 (MQ=255)

                                                                                                               tCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATcaca                                 <  2:705922/150‑1 (MQ=255)

                                                                                                               tCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATcac                                  >  2:1091577/1‑149 (MQ=255)

                                                                                                                cAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGc                               >  1:690429/1‑151 (MQ=255)

                                                                                                                 aaTTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGcc                              <  2:306326/151‑1 (MQ=255)

                                                                                                                  aTTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCa                             <  2:373825/151‑1 (MQ=255)

                                                                                                                     ccTTGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGa                           <  2:940575/150‑1 (MQ=255)

                                                                                                                       ttGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATac                        >  2:288486/1‑151 (MQ=255)

                                                                                                                       ttGCTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATac                        >  2:733740/1‑151 (MQ=255)

                                                                                                                          cTGGGCCGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACAcg                     >  1:550190/1‑151 (MQ=255)

                                                                                                                               ccGCGCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAAGGTTGTGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACAcgcccgc                <  1:1009075/151‑1 (MQ=255)

                                                                                                                                cgcgCCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACAcgcccgcc               <  2:348366/151‑1 (MQ=255)

                                                                                                                                 gcgcCGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCct              >  2:434162/1‑151 (MQ=255)

                                                                                                                                    ccGTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCctgct           >  1:248092/1‑151 (MQ=255)

                                                                                                                                      gTTTTCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCctgctg          >  1:1206810/1‑150 (MQ=255)

                                                                                                                                       ttttCCAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGCATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCctgct           >  2:1253449/1‑148 (MQ=255)

                                                                                                                                           ccAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCCTGCTGAAGCgg    <  1:1022806/151‑1 (MQ=255)

                                                                                                                                           ccAGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCCTGCTGAAGCg     <  2:624240/150‑1 (MQ=255)

                                                                                                                                             aGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCCTGCTGAAGCGGtt  >  1:545520/1‑151 (MQ=255)

                                                                                                                                             aGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCCTGCTGAAGCGGtt  >  1:187543/1‑151 (MQ=255)

                                                                                                                                             aGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCCTGCTGAAGCGGtt  >  1:1268300/1‑151 (MQ=255)

                                                                                                                                             aGACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCCTGCTGAAGCGGtt  <  1:1141221/151‑1 (MQ=255)

                                                                                                                                              gACTTCGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCCTGCTGAAGCGGtt  <  2:968708/150‑1 (MQ=255)

TTACGCGCCAACAGTAATGCCGCGTTTTTATCATTTTCCAGCTGCACATGCCCCTGAGCGTCACTGGTTGCCTGAGTCAGAGTCTGCCCTTTCTCATTTAATAAAGAGACTTCAATTCCTTGCTGGGCCGCGCCGTTTTCCAGACTTTGGGTAAAGATATCCAGACGATTGTGATAACGGTGAGCTGAAACGCCGATATCACTTAACGTAAACAGCGTCGCGGGATTACTGTAATCGTAACGTCCAGCCTGATTCATCACAGCCAGATACACGCCCGCCTGCTGAAGCGGTT  >  NC_000913/2651218‑2651509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A