Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I2 R1	121	82.0	2593086	97.7%	2533445	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,091,611	A→G	L252L (CTT→CTC)	frvB ←	putative PTS enzyme, IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,091,611	0	A	G	98.7%	262.8 / NA	75	L252L (CTT→CTC)	frvB	putative PTS enzyme, IIB component/IIC component
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (36/38); minor base C (0/1); total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

AGGAATGGTATTCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGT  >  NC_000913/4091467‑4091758

aggaATGGTATTCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGgcgc                                                                                                                                               >  1:1239625/1‑151 (MQ=255)

aggaATGGTATTCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGgcgc                                                                                                                                               >  1:154878/1‑151 (MQ=255)

 ggaATGGTATTCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGgcgc                                                                                                                                               >  2:501677/1‑150 (MQ=255)

   aaTGGTATTCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGCTTGTAATGCTTTGCCGAGGCGCACt                                                                                                                                            >  2:283682/1‑151 (MQ=255)

      ggTATTCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACttt                                                                                                                                          <  1:573856/150‑1 (MQ=255)

      ggTATTCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACttt                                                                                                                                          <  1:376693/150‑1 (MQ=255)

         aTTCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCgg                                                                                                                                      >  1:1222379/1‑151 (MQ=255)

           tCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGa                                                                                                                                     <  2:1108499/150‑1 (MQ=255)

           tCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGa                                                                                                                                     >  2:879499/1‑150 (MQ=255)

           tCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCAATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGa                                                                                                                                     <  2:342156/150‑1 (MQ=255)

               gAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAAcc                                                                                                                                  >  2:502137/1‑149 (MQ=255)

               gAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCag                                                                                                                                <  2:642690/151‑1 (MQ=255)

                aaGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAAcc                                                                                                                                  >  2:606281/1‑148 (MQ=255)

                  gTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGGAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCCAGGCGCACTTTGCGGAACCagaag                                                                                                                             <  1:261595/151‑1 (MQ=255)

                   tGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCaga                                                                                                                               >  1:591657/1‑148 (MQ=255)

                    ggAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCagaaga                                                                                                                            <  2:1182594/150‑1 (MQ=255)

                    ggAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGAc                                                                                                                           >  1:952030/1‑151 (MQ=255)

                    ggAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGAAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGAc                                                                                                                           >  2:56991/1‑151 (MQ=255)

                         agcCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCAGTTTGCGGAACCAGAAGACGAAAt                                                                                                                      <  1:983354/151‑1 (MQ=255)

                           cccgcccATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATa                                                                                                                     <  1:831606/150‑1 (MQ=255)

                              gcccATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGcc                                                                                                                  >  1:972776/1‑150 (MQ=255)

                                     aGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCa                                                                                                          <  2:527797/151‑1 (MQ=255)

                                        tCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGcc                                                                                                       >  2:586002/1‑151 (MQ=255)

                                         cGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGccc                                                                                                      <  1:208547/151‑1 (MQ=255)

                                           gACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCa                                                                                                    <  2:585453/151‑1 (MQ=255)

                                           gACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCa                                                                                                    <  1:724605/151‑1 (MQ=255)

                                              aTCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGc                                                                                                  >  1:315557/1‑150 (MQ=255)

                                              aTCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGc                                                                                                  >  2:199979/1‑150 (MQ=255)

                                                cACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGcacc                                                                                               >  2:491584/1‑151 (MQ=255)

                                                cACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGcacc                                                                                               >  2:390861/1‑151 (MQ=255)

                                                 aCCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGcacca                                                                                              <  2:154879/151‑1 (MQ=255)

                                                     gCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACcgc                                                                                          >  1:1274516/1‑151 (MQ=255)

                                                        cGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGcc                                                                                       >  2:20970/1‑151 (MQ=255)

                                                          aTAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCg                                                                                      >  2:693255/1‑150 (MQ=255)

                                                            aaCGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAgg                                                                                   <  1:282604/151‑1 (MQ=255)

                                                               gttggaaGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGTTCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGaa                                                                                 >  1:203978/6‑150 (MQ=255)

                                                                    aaGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATgcca                                                                            <  1:258923/150‑2 (MQ=255)

                                                                          gCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGa                                                                     <  2:243154/151‑1 (MQ=255)

                                                                            aCGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGCTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGa                                                                     >  1:652291/1‑149 (MQ=255)

                                                                             cGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTg                                                                  <  2:1186275/151‑1 (MQ=255)

                                                                                  aaaaCCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGc                                                             >  1:979831/1‑151 (MQ=255)

                                                                                   aaaCCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGcc                                                            >  1:937707/1‑151 (MQ=255)

                                                                                       cagcagAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGagc                                                        <  1:789056/151‑1 (MQ=255)

                                                                                           agagTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGagaagc                                                     <  1:350148/150‑1 (MQ=255)

                                                                                             agTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCt                                                  >  2:1237509/1‑151 (MQ=255)

                                                                                               tAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCttt                                                >  1:379548/1‑151 (MQ=255)

                                                                                               tAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCttt                                                >  2:526808/1‑151 (MQ=255)

                                                                                                aacgaacgGGATCAGCATTGAACCGAGCAGCGGTTGAATTGATTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATAGCCAGTCCCAGCACCACCGCGCCGAGGAAACCCGCATCCGACTGGCTGGCGAACAGCGCttt                                                >  1:148930/1‑150 (MQ=255)

                                                                                                   gaacgGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCg                                            >  1:965508/1‑151 (MQ=255)

                                                                                                     acgGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGtt                                          >  1:1131061/1‑151 (MQ=255)

                                                                                                          aTCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCa                                     <  1:550063/151‑1 (MQ=255)

                                                                                                             aGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGAt                                  <  1:280705/151‑1 (MQ=255)

                                                                                                              gCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAGTCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTGTGTCGTTCGCCAGATa                                 <  1:388487/151‑1 (MQ=255)

                                                                                                                  tGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCa                              <  2:748914/150‑1 (MQ=255)

                                                                                                                  tGAACCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCa                              >  2:384774/1‑150 (MQ=255)

                                                                                                                    aaCCGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAAc                           >  1:200424/1‑151 (MQ=255)

                                                                                                                      ccGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAAcc                          <  2:233951/150‑1 (MQ=255)

                                                                                                                       cGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAg                        <  2:1199067/151‑1 (MQ=255)

                                                                                                                       cGAGCAGCGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAg                        <  2:1091922/151‑1 (MQ=255)

                                                                                                                          gcagcGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGaaa                     <  2:1036722/151‑1 (MQ=255)

                                                                                                                           cagcGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAAc                    >  2:408872/1‑151 (MQ=255)

                                                                                                                           cagcGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAAc                    <  1:245830/151‑1 (MQ=255)

                                                                                                                             gcGGTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGc                  >  2:874464/1‑151 (MQ=255)

                                                                                                                               ggTTGTAATGCTTTGCCGAGGCGCCCTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCCCCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCg                <  2:693831/151‑1 (MQ=255)

                                                                                                                               ggTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGcc                 <  1:191720/150‑1 (MQ=255)

                                                                                                                               ggTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCg                <  1:1216215/151‑1 (MQ=255)

                                                                                                                                gTTGTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCgg               <  2:952066/151‑1 (MQ=255)

                                                                                                                                   gTAATGCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCCCCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGc            <  2:1254582/151‑1 (MQ=255)

                                                                                                                                        gCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCGGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAACCGCCGGAGCGaaa        <  1:575514/150‑1 (MQ=255)

                                                                                                                                        gCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGaaa        <  1:504823/150‑1 (MQ=255)

                                                                                                                                        gCTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGaa         >  2:606247/1‑149 (MQ=255)

                                                                                                                                         cTTTGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGc      >  1:1199335/1‑151 (MQ=255)

                                                                                                                                            tGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCg    <  2:376002/150‑1 (MQ=255)

                                                                                                                                            tGCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCg    <  1:973311/150‑1 (MQ=255)

                                                                                                                                             gCCGAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGt  <  2:315577/151‑1 (MQ=255)

AGGAATGGTATTCAGGAAGTGGAGCAGCCCGCCCATGAGGTCGGACATCACCGGCCCGATAACGTAGTAAGTGAGCACGCCGAAAACCAGCAGAGTAACGAACGGGATCAGCATTGAACCGAGCAGCGGTTGTAATGCTTTGCCAAGGCGCACTTTGCGGAACCAGAAGACGAAATAGCCAATCGCCAGCCCCAGCACCACCGCGCCGAGGAATCCCGCACCCGACTGGGTGCCGAGCAGCGCTTTGTCGTTCGCCAGATAGCAAACCAGAAACGCCGGAGCGAAAGCCGGT  >  NC_000913/4091467‑4091758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A