Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F50 I2 R1 139 64.5 2139856 92.7% 1983646 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,066,324 A→G Q247R (CAG→CGG)  agp → glucose‑1‑phosphatase/inositol phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,066,3240AG98.1% 187.2 / NA 52Q247R (CAG→CGG) agpglucose‑1‑phosphatase/inositol phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (25/26);  minor base T (0/1);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTTATATCGACAAAGCTCTGGT  >  NC_000913/1066177‑1066471
                                                                                                                                                   |                                                                                                                                                   
agaAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCggg                                                                                                                                                   >  1:997586/1‑150 (MQ=255)
  aaaCAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTgg                                                                                                                                                >  1:407242/1‑151 (MQ=255)
  aaaCAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTg                                                                                                                                                 <  1:991115/150‑1 (MQ=255)
           tgtTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTgggg                                                                                                                                         >  1:676923/1‑149 (MQ=255)
              tCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGaa                                                                                                                                     <  2:816992/150‑1 (MQ=255)
                   tgtggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGaaatcaa                                                                                                                                >  1:604545/2‑150 (MQ=255)
                   ggtggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGaaatcaa                                                                                                                                >  1:196160/1‑150 (MQ=255)
                    gtggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGaaatcaa                                                                                                                                <  2:956716/149‑1 (MQ=255)
                     tggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGaaatcaaatc                                                                                                                             >  2:507953/1‑151 (MQ=255)
                      ggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCt                                                                                                                            <  1:531441/151‑1 (MQ=255)
                       gATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTg                                                                                                                           >  1:554530/1‑151 (MQ=255)
                       gATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTg                                                                                                                           >  2:395900/1‑151 (MQ=255)
                        aTGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTg                                                                                                                           >  2:586811/1‑150 (MQ=255)
                              aaaaTACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACcagc                                                                                                                     >  1:612702/1‑150 (MQ=255)
                                   aCCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTgg                                                                                                                <  2:1013153/150‑1 (MQ=255)
                                        tAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGgtg                                                                                                          >  2:59263/1‑151 (MQ=255)
                                                   aTCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGc                                                                                                <  2:309209/150‑1 (MQ=255)
                                                      aacaaGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTaa                                                                                            <  1:818611/151‑1 (MQ=255)
                                                      aacaaGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTa                                                                                             >  2:501864/1‑150 (MQ=255)
                                                      aacaaGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTa                                                                                             >  2:469049/1‑150 (MQ=255)
                                                           gAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTaaaa                                                                                          <  2:380811/148‑1 (MQ=255)
                                                              ccAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCt                                                                                    <  2:631526/151‑1 (MQ=255)
                                                                aGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTAc                                                                                  <  1:1010297/151‑1 (MQ=255)
                                                                   tgtTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTAcc                                                                                 >  1:344131/1‑149 (MQ=255)
                                                                      ttCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGa                                                                             <  2:997844/150‑1 (MQ=255)
                                                                      ttCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGAc                                                                            >  2:719234/1‑151 (MQ=255)
                                                                        ccGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAg                                                                          <  2:837468/151‑1 (MQ=255)
                                                                           ggCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCt                                                                       <  1:970075/151‑1 (MQ=255)
                                                                               gCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGttt                                                                   <  1:32934/151‑1 (MQ=255)
                                                                                            aaCTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAg                                                      <  2:336948/151‑1 (MQ=255)
                                                                                                cGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTg                                                    >  2:522981/1‑149 (MQ=255)
                                                                                                    ggTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGgcgcg                                               <  2:363021/150‑1 (MQ=255)
                                                                                                          tGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGtt                                        >  1:862683/1‑151 (MQ=255)
                                                                                                          tGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGtt                                        >  1:761937/1‑151 (MQ=255)
                                                                                                          tGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGtt                                        >  2:686055/1‑151 (MQ=255)
                                                                                                           gCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTg                                       <  1:140502/151‑1 (MQ=255)
                                                                                                            cGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGc                                      >  2:892677/1‑151 (MQ=255)
                                                                                                            cGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGc                                      <  2:214376/151‑1 (MQ=255)
                                                                                                              tttACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCg                                     >  1:595969/1‑150 (MQ=255)
                                                                                                               ttACTTTGCAATATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGa                                    <  2:1013395/150‑1 (MQ=255)
                                                                                                                        aaTATTACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTgg                           >  1:645778/1‑150 (MQ=255)
                                                                                                                           atTACGACGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTc                         >  2:771416/1‑149 (MQ=255)
                                                                                                                             tACGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGtt                     >  2:936140/1‑151 (MQ=255)
                                                                                                                              aCGAAGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGtt                     <  1:862427/150‑1 (MQ=255)
                                                                                                                                  aGGTTTTGCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCGGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTtata                  <  2:969585/149‑1 (MQ=255)
                                                                                                                                  aGGTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTTATATc                <  1:359285/151‑1 (MQ=255)
                                                                                                                                   ggTTTTCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTTATATCg               <  1:748337/151‑1 (MQ=255)
                                                                                                                                     ttttCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTTATATCGAc             >  2:137473/1‑151 (MQ=255)
                                                                                                                                      tttCCGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTTATATCGAc             >  2:226807/1‑150 (MQ=255)
                                                                                                                                         ccGATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTTATATCGACAAAg         <  1:685211/151‑1 (MQ=255)
                                                                                                                                           gATGGATCTGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCTAAGCTTAAAGACGGTTACCAGGACAGCCTGGTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTTATATCGACAAAg         <  2:681817/149‑1 (MQ=255)
                                                                                                                                           gATGGATCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTTATATCGACAAAGct       <  2:960351/151‑1 (MQ=255)
                                                                                                                                                aTCGGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTTATATCGACAAAGCTCTGGt  <  1:116119/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCGCGCAATGTTGCGAAACCGCTGGTCAGTTATATCGACAAAGCTCTGGT  >  NC_000913/1066177‑1066471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A