Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F50 I2 R1 139 64.5 2139856 92.7% 1983646 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,297,484 G→A R219C (CGC→TGC)  yraQ ← putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,297,4840GA98.3% 208.2 / ‑4.3 58R219C (CGC→TGC) yraQputative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (32/25);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

CATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCC  >  NC_000913/3297338‑3297630
                                                                                                                                                  |                                                                                                                                                  
cATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTa                                                                                                                                                <  2:364151/151‑1 (MQ=255)
 aTCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTa                                                                                                                                                >  1:719404/1‑150 (MQ=255)
  tCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTaa                                                                                                                                               >  1:850712/1‑150 (MQ=255)
   cGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTaaa                                                                                                                                              <  1:118891/150‑1 (MQ=255)
     ccaccaCCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTaaaaa                                                                                                                                            >  1:10173/1‑150 (MQ=255)
       accaccCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAAcc                                                                                                                                         >  1:127136/1‑151 (MQ=255)
        ccacccacATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAAc                                                                                                                                          <  2:578065/149‑1 (MQ=255)
          acccacATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGc                                                                                                                                      >  1:20014/1‑151 (MQ=255)
          acccacATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGc                                                                                                                                      >  2:53374/1‑151 (MQ=255)
              acaTCACGCTGTTACCGACAGGACCATCGGCATGGGGGAATAATCAGACGCGAGCGGCACCCAACACCAGAACTGCAAGGAAGTAAACCGGGAGCGCTTTCCATAAAAGCGCCCATAGCGCCCTGCCCTCGCAGCTAAAAAACCCGcc                                                                                                                                     >  2:935015/1‑148 (MQ=255)
                        gTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACCGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGt                                                                                                                        >  1:74980/1‑151 (MQ=255)
                          tATCGACAGCACCATCGGCATGGGGGAATAAACAGACGCGAACGGCACACAACACCAGTACTGCAAGGATGGAAACCGGGATCGTACTCCAGAAAAGCGTCAATAGAGCCCTGCCCCAGCAGCTAAAAAACCCGCCGTGTGCTTCCGGTa                                                                                                                       >  2:406145/1‑150 (MQ=255)
                           aTCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTAt                                                                                                                      <  1:967964/150‑1 (MQ=255)
                           aTCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGCACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATg                                                                                                                     >  1:435147/1‑151 (MQ=255)
                               aCAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCa                                                                                                                  <  1:301627/150‑1 (MQ=255)
                                 aGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGc                                                                                                                               >  2:512568/1‑135 (MQ=255)
                                 aGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGc                                                                                                                               <  1:512595/135‑1 (MQ=255)
                                   cagcATCGGCATGGGGGATGATCCAGACGCGAGAGACACCCAACACCAGGACTGAAAGGATGGAAATCGTGATTGTAATCCAGAATAGCGTCCATAGCGGCAAGCCCCAGAAGAGAAAAAATCCGCCCTGTGCTTCCGGTATGTaaa                                                                                                                 <  2:7363/144‑1 (MQ=255)
                                   cACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTc                                                                                                             <  1:8182/151‑1 (MQ=255)
                                    aCCATCGGCATGGGGGAATAACCAGACGCGAGCGGCAACCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTc                                                                                                             >  2:153810/1‑150 (MQ=255)
                                     ccATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGa                                                                                                           <  1:608996/151‑1 (MQ=255)
                                         cGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCgg                                                                                                       <  1:1069430/151‑1 (MQ=255)
                                         cGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCgg                                                                                                       <  1:1008541/151‑1 (MQ=255)
                                              tGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCt                                                                                                  >  2:980635/1‑151 (MQ=255)
                                               gggggAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTg                                                                                                 <  2:1040958/151‑1 (MQ=255)
                                                ggggAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGc                                                                                                <  1:991551/151‑1 (MQ=255)
                                                  ggAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGt                                                                                              >  1:229669/1‑151 (MQ=255)
                                                   gAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGtt                                                                                             <  1:642318/151‑1 (MQ=255)
                                                      tAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGc                                                                                          >  2:145557/1‑151 (MQ=255)
                                                       aaCCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCg                                                                                         <  1:898841/151‑1 (MQ=255)
                                                           aGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGgtgt                                                                                     >  1:800553/1‑151 (MQ=255)
                                                            gACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGtt                                                                                    >  2:707455/1‑151 (MQ=255)
                                                             aCGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGttt                                                                                   <  1:515468/151‑1 (MQ=255)
                                                                              cacCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGc                                                                  >  1:592253/1‑151 (MQ=255)
                                                                                ccAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCAc                                                                >  2:502834/1‑151 (MQ=255)
                                                                                  aGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCAcc                                                               >  2:690886/1‑150 (MQ=255)
                                                                                  aGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCa                                                              <  1:115303/151‑1 (MQ=255)
                                                                                  aGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCa                                                              <  1:506249/151‑1 (MQ=255)
                                                                                   gTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCAcc                                                               >  2:552860/1‑149 (MQ=255)
                                                                                     aCTGCAAGGATGTAATCCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTATAACACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGc                                                                  <  2:401368/144‑1 (MQ=255)
                                                                                     aCTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCg                                                           <  1:372792/151‑1 (MQ=255)
                                                                                      cTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGt                                                          >  2:999291/1‑151 (MQ=255)
                                                                                        gCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTc                                                         <  1:23629/150‑1 (MQ=255)
                                                                                         cAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGc                                                       >  1:11880/1‑151 (MQ=255)
                                                                                              aTGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGATTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATc                                                   >  1:984653/1‑150 (MQ=255)
                                                                                                  aaaCCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCg                                                           >  2:445309/1‑138 (MQ=255)
                                                                                                  aaaCCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCg                                                           <  1:445335/138‑1 (MQ=255)
                                                                                                          aTCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATc                                       >  2:680823/1‑150 (MQ=255)
                                                                                                          aTCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCa                                      <  1:106231/151‑1 (MQ=255)
                                                                                                                        aaGCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGccacc                        >  1:571106/1‑151 (MQ=255)
                                                                                                                          gCGTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGccacc                        >  2:31203/1‑149 (MQ=255)
                                                                                                                            gTCCATAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGAc                    >  1:858031/1‑151 (MQ=255)
                                                                                                                                aTAGCGCCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAAt                >  1:504368/1‑151 (MQ=255)
                                                                                                                                    cgcCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATcgcc            >  1:149753/1‑151 (MQ=255)
                                                                                                                                     gcCCTGCCCCAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATcgccg           <  2:471897/151‑1 (MQ=255)
                                                                                                                                            cccAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAAcc    >  1:812591/1‑151 (MQ=255)
                                                                                                                                             ccAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAAccc   <  1:210524/151‑1 (MQ=255)
                                                                                                                                             ccAGCAGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAAccc   >  1:1000490/1‑151 (MQ=255)
                                                                                                                                               agcagcTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAAcccc  >  1:261294/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CATCGCCACCACCCACATCAGGCTGTTATCGACAGCACCATCGGCATGGGGGAATAACCAGACGCGAGCGGCACCCAACACCAGTACTGCAAGGATGTAAACCGGGATCGTACTCCAGAAAAGCGTCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCC  >  NC_000913/3297338‑3297630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A