Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,542,564	C→T	H720Y (CAT→TAT)	feoB →	ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,542,564	0	C	T	98.6%	272.9 / NA	71	H720Y (CAT→TAT)	feoB	ferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (36/33); minor base G (0/1); total (37/34)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTGCGCAGCCAGCACCA  >  NC_000913/3542416‑3542711

cGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTAt                                                                                                                                                   >  1:466751/1‑151 (MQ=255)

 gTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATc                                                                                                                                                  <  2:198116/151‑1 (MQ=255)

     tgctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAAc                                                                                                                                              >  2:727842/1‑151 (MQ=255)

         gtatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTa                                                                                                                                           <  2:451944/150‑1 (MQ=255)

          tatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTAGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTAc                                                                                                                                          >  2:72479/1‑150 (MQ=255)

           atgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTAc                                                                                                                                          >  2:469372/1‑149 (MQ=255)

           atgtaCCATGTATCTCGGAGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCATAATCCAACTTa                                                                                                                                           >  2:717753/1‑148 (MQ=255)

            tgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGc                                                                                                                                       >  1:817653/1‑151 (MQ=255)

              taCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCt                                                                                                                                     >  2:874172/1‑151 (MQ=255)

              taCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCt                                                                                                                                     >  2:283305/1‑151 (MQ=255)

                ccATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTgg                                                                                                                                   >  1:549568/1‑151 (MQ=255)

                 cATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGgt                                                                                                                                  <  2:966882/151‑1 (MQ=255)

                  aTGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGgtg                                                                                                                                 >  1:678351/1‑151 (MQ=255)

                     tATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGc                                                                                                                              >  2:35985/1‑151 (MQ=255)

                        ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCAtt                                                                                                                           >  2:820690/1‑151 (MQ=255)

                         tcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTc                                                                                                                          >  2:470199/1‑151 (MQ=255)

                          cGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCAtt                                                                                                                           <  2:1053773/149‑1 (MQ=255)

                          cGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCAtt                                                                                                                           <  1:1067329/149‑1 (MQ=255)

                          cGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTc                                                                                                                          <  2:136826/150‑1 (MQ=255)

                          cGGTGATAGCGGCTATCGTACGTGACTCAAGCCGCGGCTGGATGGGCTTCTCCCTCCTGAGGGGGCTGGATGACCCTTACTCACTGGCAACAATGGTCAATCAGGTCGCCAGCTACAGTCAGTATCCACATTACAGCCTGGTGTGCATTCt                                                                                                                         >  2:197097/1‑151 (MQ=255)

                           ggTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTg                                                                                                                        >  1:420142/1‑151 (MQ=255)

                           ggTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTg                                                                                                                        >  1:934272/1‑151 (MQ=255)

                              gATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCg                                                                                                                     >  1:894867/1‑151 (MQ=255)

                                        aTCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGt                                                                                                           <  1:551639/151‑1 (MQ=255)

                                        aTCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGt                                                                                                           <  2:131010/151‑1 (MQ=255)

                                            cccGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTaa                                                                                                       <  2:604354/151‑1 (MQ=255)

                                              cGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAAc                                                                                                      >  2:167700/1‑150 (MQ=255)

                                               gTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACAt                                                                                                    >  1:5416/1‑151 (MQ=255)

                                                 gAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATc                                                                                                   <  1:500951/150‑1 (MQ=255)

                                                  aaTCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGt                                                                                                 <  1:33412/151‑1 (MQ=255)

                                                      aaGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGtt                                                                                             >  1:770568/1‑151 (MQ=255)

                                                       aGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGtt                                                                                             >  2:823957/1‑150 (MQ=255)

                                                        gacGTGGCTGGATGGGCTTCTCCATCCTGGGGGGGCTGAATATCGATTAGTAACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTGATCCCGTTTAACAACGTgg                                                                                               >  2:283046/3‑147 (MQ=255)

                                                        gCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTAt                                                                                           <  1:60605/151‑1 (MQ=255)

                                                          cGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCg                                                                                         <  2:529772/151‑1 (MQ=255)

                                                           gTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCgg                                                                                        >  2:12476/1‑151 (MQ=255)

                                                              gCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTct                                                                                     <  1:175313/151‑1 (MQ=255)

                                                              gCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTct                                                                                     <  1:504585/151‑1 (MQ=255)

                                                               cTGGATGGGCTTCTCCATCCCGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTct                                                                                     >  2:785991/1‑150 (MQ=255)

                                                                 ggATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTTCAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTctgct                                                                                  <  2:933469/151‑1 (MQ=255)

                                                                 ggATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTctgct                                                                                  >  1:1007633/1‑151 (MQ=255)

                                                                  gATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTctgctg                                                                                 <  2:336696/151‑1 (MQ=255)

                                                                  gATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTctgctg                                                                                 <  1:177222/151‑1 (MQ=255)

                                                                     gggCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTgcgc                                                                              <  1:235984/151‑1 (MQ=255)

                                                                             ccATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCcgcgc                                                                         <  1:134407/148‑1 (MQ=255)

                                                                              cATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGt                                                                     >  1:419401/1‑151 (MQ=255)

                                                                                 ccTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAg                                                                   <  1:161779/150‑1 (MQ=255)

                                                                                           cTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGat                                                         >  2:536287/1‑150 (MQ=255)

                                                                                              aaTATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGGGGATATCg                                                     >  2:365429/1‑151 (MQ=255)

                                                                                                tatCGCTTACTCTCTGGCGACGTTGTTCTATCAGGTCGCCCGCTACAGTCAGTATCCACCTTACAGCCTGGTGTGCATTCCGGCGGTTAACTTTTTTAACATCGAGGTTATTGGTCTGCTGCGCCGCGAGCCTGGCCGGGTGGGTATCGa                                                    >  2:341411/1‑150 (MQ=255)

                                                                                                   cGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAAct                                                 <  2:768472/150‑1 (MQ=255)

                                                                                                           cACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAAc                                        >  1:885358/1‑151 (MQ=255)

                                                                                                               ggCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAAccc                                      <  2:256169/149‑1 (MQ=255)

                                                                                                               ggCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCg                                     <  1:798483/150‑1 (MQ=255)

                                                                                                                 cAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCa                                   >  1:552340/1‑150 (MQ=255)

                                                                                                                  aaCATTGTTCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAg                                 <  1:710241/151‑1 (MQ=255)

                                                                                                                          tCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTaa                          <  1:951592/150‑1 (MQ=255)

                                                                                                                          tCTATCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTa                           >  2:839402/1‑149 (MQ=255)

                                                                                                                             aTCAGGTCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAg                         <  2:1807/148‑1 (MQ=255)

                                                                                                                                   tCGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTtgctg                >  2:975632/1‑151 (MQ=255)

                                                                                                                                    cGCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTtgctg                <  2:25013/150‑1 (MQ=255)

                                                                                                                                     gCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTgcg              <  2:540639/151‑1 (MQ=255)

                                                                                                                                     gCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTgcg              >  1:136835/1‑151 (MQ=255)

                                                                                                                                     gCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTgcg              >  1:619023/1‑151 (MQ=255)

                                                                                                                                     gCCAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCGGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGTGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTtgctgc               <  2:681388/150‑1 (MQ=255)

                                                                                                                                       cAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGGTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTgcgc             >  2:3093/1‑150 (MQ=255)

                                                                                                                                       cAGCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTATTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTGCGca            <  1:255125/151‑1 (MQ=255)

                                                                                                                                         gCTACAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTGCGcagc          <  2:38127/151‑1 (MQ=255)

                                                                                                                                            aCAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTGCGcagccag       <  2:766258/151‑1 (MQ=255)

                                                                                                                                            aCAGTCAGTATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTGCGcagcc         >  1:853075/1‑149 (MQ=255)

                                                                                                                                                 cAGGATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTGCGCAGCCAGcacca  <  2:976250/151‑1 (MQ=255)

CGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTTATCCTGTTTAACATCGTGGTTATCGGTCTGCTGCGCCGCGCGCGTAGCCGGGTGGATATCGAACTGCTGGCAACCCGCAAGTCGGTAAGCAGTTGCTGCGCAGCCAGCACCA  >  NC_000913/3542416‑3542711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A