Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F50 I2 R1 139 64.5 2139856 92.7% 1983646 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,601,428 A→G G30G (GGT→GGC)  dnaT ← DNA biosynthesis protein (primosomal protein I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,601,4280AG100.0% 152.5 / NA 44G30G (GGT→GGC) dnaTDNA biosynthesis protein (primosomal protein I)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/29);  total (15/29)

TGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACA  >  NC_000913/4601285‑4601575
                                                                                                                                               |                                                                                                                                                   
tGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCtt                                                                                                                                              >  2:28220/1‑151 (MQ=255)
  cGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTc                                                                                                                                             >  2:595987/1‑150 (MQ=255)
   ggTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTc                                                                                                                                             >  1:375917/1‑149 (MQ=255)
               agTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGa                                                                                                                                >  1:1057032/1‑150 (MQ=255)
                  tGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACg                                                                                                                             <  2:362829/150‑1 (MQ=255)
                    aTCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGtt                                                                                                                          <  2:699843/151‑1 (MQ=255)
                      cGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTg                                                                                                                        <  2:589433/151‑1 (MQ=255)
                                     cGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCa                                                                                                          <  1:637094/150‑1 (MQ=255)
                                     cGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCa                                                                                                          <  2:381281/150‑1 (MQ=255)
                                        ttCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGc                                                                                                      >  1:265224/1‑151 (MQ=255)
                                         tCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGATCGGTTTGGTGATCGTGTTCCAGGGc                                                                                                      <  2:503756/150‑1 (MQ=255)
                                         tCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGATTGGTGATCGTGTACCAGGGc                                                                                                      >  1:809967/1‑150 (MQ=255)
                                              gACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAAt                                                                                                <  2:54578/151‑1 (MQ=255)
                                              gACGCGCCAGCTTTTCTTCCAGCGCCAGCACTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAAt                                                                                                >  1:381737/1‑151 (MQ=255)
                                                  cgcCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCa                                                                                            <  2:330947/151‑1 (MQ=255)
                                                               tcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCggg                                                                                <  2:408625/150‑1 (MQ=255)
                                                               tcCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCgg                                                                                 <  2:513386/149‑1 (MQ=255)
                                                                ccAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCgggg                                                                               <  2:146029/150‑1 (MQ=255)
                                                                   gcgcCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCTCGCCGCCTTCAGCTTGTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGGCAATCCCAACGACGACCGGGGTCaa                                                                           <  2:158643/151‑1 (MQ=255)
                                                                       cagcagTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAAct                                                                       <  2:44378/151‑1 (MQ=255)
                                                                              tCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGa                                                                  >  2:657271/1‑149 (MQ=255)
                                                                                aGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGAc                                                              >  1:881617/1‑151 (MQ=255)
                                                                                        gtgcaggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGtt                                                      <  2:273379/151‑1 (MQ=255)
                                                                                           caggcgtgACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTtc                                                    >  2:993152/1‑150 (MQ=255)
                                                                                             ggcgtgACGGCGTAAAACGCCGGGGCATTGTTAGCAAATACGGCAGCCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGTCGGCAATACCAACGACGTCCGGGGTGAAAACTCTGGAAGACATACTGTTtctc                                                  <  2:540326/150‑1 (MQ=255)
                                                                                                gtgGCGGCATAAAACGCCGGGGGATTGTTAACAAAGACGGCAACCACGGCGCCTTCAGCTTTTGCCAGAACGTTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCaa                                                <  2:520244/149‑1 (MQ=255)
                                                                                                  gACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAata                                             <  1:294265/150‑1 (MQ=255)
                                                                                                      gCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGa                                         >  1:872481/1‑150 (MQ=255)
                                                                                                       cATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAAc                                       <  2:667029/151‑1 (MQ=255)
                                                                                                               gCCGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACg                               <  2:275059/151‑1 (MQ=255)
                                                                                                                ccGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACga                              <  1:790329/151‑1 (MQ=255)
                                                                                                                 cGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACgag                             <  2:737462/151‑1 (MQ=255)
                                                                                                                 cGGGGCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACga                              <  2:729658/150‑1 (MQ=255)
                                                                                                                   gggCATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAGCTCTGGAAGACAGACTGTTTCTCAAATATGGAACGATGAACgaga                            <  2:218186/150‑1 (MQ=255)
                                                                                                                      cATTGTTAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAggg                        >  1:743330/1‑151 (MQ=255)
                                                                                                                           ttAGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCTGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGAt                    >  1:1022593/1‑150 (MQ=255)
                                                                                                                             aGCAAATACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGTGATAAc                 <  1:411989/151‑1 (MQ=255)
                                                                                                                                aaaTACGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACttt              <  2:188185/151‑1 (MQ=255)
                                                                                                                                    aCGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTtata          <  1:868606/151‑1 (MQ=255)
                                                                                                                                    aCGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTtata          <  1:892213/151‑1 (MQ=255)
                                                                                                                                    aCGGCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTtat           >  1:873587/1‑150 (MQ=255)
                                                                                                                                      ggCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGt        <  1:56101/151‑1 (MQ=255)
                                                                                                                                      ggCAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGt        <  2:786121/151‑1 (MQ=255)
                                                                                                                                        cAACCACGCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTa       >  2:65024/1‑150 (MQ=255)
                                                                                                                                            cacgCCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAacaca  <  2:207998/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACA  >  NC_000913/4601285‑4601575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A