Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 647,640 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583647,6400AT99.2% 573.3 / NA 244intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (118/123);  minor base G (2/0);  total (121/123)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583647,6410CG100.0% 575.0 / NA 244intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (121/123);  total (121/123)
*CP042583647,6420TA99.6% 574.8 / NA 244intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (120/123);  total (121/123)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 261 total aligned reads displayed.

TATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGACTAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGACTGGAC‑‑GTGTGACTGTGACTGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATACTAGA  >  CP042583/647370‑647923
                                                                                                                                                                                                                                                                                 |||                                                                                                                                                                                                                                                                                         
       ttCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GGGGGGGAGTGTGAGTGCGCCATGACGAGTc                                                                                                                                                                                                                                                                    >  2:564031/1‑289 (MQ=255)
        tCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCa                                                                                                                                                                                                                                                                   >  1:117175/1‑289 (MQ=255)
        tCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCa                                                                                                                                                                                                                                                                   >  1:1248413/1‑289 (MQ=255)
        tCAAAAACGCCGGGGGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGAGAATGGTGGGAGGACGACGTGAAAATGGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCa                                                                                                                                                                                                                                                                   <  2:1245915/289‑1 (MQ=255)
           caAACGCCGCGGGGGTGGGCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATcc                                                                                                                                                                                                                                                                <  2:610657/289‑1 (MQ=255)
                  cgcgAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGAATTATTATCTTCTGTCAGGTGTGAGGACAGGTGTGAGTGTGA‑‑GTGCGCCATGACGAGTCATCCCTTAatt                                                                                                                                                                                                                                                         >  1:2082426/1‑289 (MQ=255)
                      aGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGCCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAattatt                                                                                                                                                                                                                                                      <  1:330825/288‑1 (MQ=255)
                             tCAGTGTTAGCCAACCGAAAGGGTGGCGAATGGGGGGAGGCGTACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGAAGGGCGGAAGTATCGGTAAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGCCTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACtt                                                                                                                                                                                                                                               <  2:112231/288‑1 (MQ=255)
                             tCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACtt                                                                                                                                                                                                                                               <  2:1533044/288‑1 (MQ=255)
                             tCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACtt                                                                                                                                                                                                                                               <  1:416882/288‑1 (MQ=255)
                                   ttAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTa                                                                                                                                                                                                                                        <  1:880684/289‑1 (MQ=255)
                                   ttAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTa                                                                                                                                                                                                                                        <  2:217601/289‑1 (MQ=255)
                                     cgCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATATTATGGCAGGTGTGAGGAC‑‑GCGTGTGAGTGTGAGTGCGCCATGACGGATCATCCCTTAATTATCATCACTTAACTTTATg                                                                                                                                                                                                                                      >  2:2120536/2‑289 (MQ=255)
                                     aGCCAACCGAAAGGGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATg                                                                                                                                                                                                                                      <  2:1255570/289‑1 (MQ=255)
                                           ccGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGCGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATTAcatca                                                                                                                                                                                                                                >  1:1928913/1‑289 (MQ=255)
                                           ccGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAattat                                                                                                                                                                                                                                                       <  1:2076097/266‑1 (MQ=255)
                                           ccGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGAcatct                                                                                                                                                                                                                                >  1:1933873/1‑288 (MQ=255)
                                           ccGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGAcatca                                                                                                                                                                                                                                >  2:831536/1‑289 (MQ=255)
                                               aaGTGGGGCGAATGGTGGGAGCACGACGTGAACATTTCACGGCGATTAGGGCGAAATGCAGGGCGGCAGTATCGGTCAACCTATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTCATTATTCTCACCTAACTTTATGACATCATACt                                                                                                                                                                                                                            <  1:1834024/289‑1 (MQ=255)
                                                  tgtgGCGAATGGTGGGAGGACGACGTGAAAATGGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGGCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGTGCCATGACGAGTCa                                                                                                                                                                                                                                                                   <  1:1675135/247‑1 (MQ=255)
                                                         aaTGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGaa                                                                                                                                                                                                                  >  1:1078466/1‑289 (MQ=255)
                                                            ggtggGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCACTTAATTACTCTCACTTAACTTTATGACCTCATACTTCTTTTAGAAGtt                                                                                                                                                                                                               >  2:1553783/1‑288 (MQ=255)
                                                                ggaggaCGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGaaa                                                                                                                                                                                                            <  2:1078466/288‑1 (MQ=255)
                                                                ggaggaCGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGaaa                                                                                                                                                                                                            <  1:1129180/288‑1 (MQ=255)
                                                                ggaggaCGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGaaa                                                                                                                                                                                                            <  1:564031/288‑1 (MQ=255)
                                                                      cgacgtgaaaaTTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTaaa                                                                                                                                                                                                     >  2:1715967/9‑289 (MQ=255)
                                                                        acgtgaaaaTTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTc                                                                                                                                                                                                                                                                    >  1:2252680/7‑224 (MQ=255)
                                                                        acgtgaaaaTTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACt                                                                                                                                                                                                                            <  1:1015400/258‑1 (MQ=255)
                                                                        acgtgaaaaTTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTACTATCTTCTGGCAGGGGTGAGGAC‑‑GTGTGGGAGTGTGAGGTCGCCATGACGTGTCATCCCTTAATTTTTCTCCCTTCACTTTATGAACTCATACTGCTTTTAGACGTTCAACATTAAAAg                                                                                                                                                                                                   >  2:2233922/7‑289 (MQ=255)
                                                                            gaaaaTTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGgag                                                                                                                                                                                               <  2:674131/287‑1 (MQ=255)
                                                                                    cACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGTGAGAGACTcc                                                                                                                                                                                        >  1:1611575/1‑288 (MQ=255)
                                                                                     aCGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCg                                                                                                                                                                                       <  1:2174622/288‑1 (MQ=255)
                                                                                     aCGGCGAGTAGGGCGAAAAGCAGGGCGGCAATATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAACGAAAACCACCAA‑CGACTTCTACCAGCAGAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGc                                                                                                                                                                                      <  2:202864/289‑1 (MQ=255)
                                                                                         cGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATa                                                                                                                                                                                                                              >  1:2018906/1‑245 (MQ=255)
                                                                                                   gAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTgg                                                                                                                                                                        <  1:1605301/289‑1 (MQ=255)
                                                                                                          aGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGTTCTCCCATTATTGGCTAtttt                                                                                                                                                                 >  1:971748/1‑289 (MQ=255)
                                                                                                                    tATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGTAGAGACTCCGACGTCCCCTTATTGGCCATTTTGCAGGGtt                                                                                                                                                         >  2:1034068/1‑287 (MQ=255)
                                                                                                                    tATCGGTAGACATATGACCCGCATCGGCCAGCAAAGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTa                                                                                                                                                        <  2:928802/288‑1 (MQ=255)
                                                                                                                     aTCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACt                                                                                                                                                      <  1:936509/289‑1 (MQ=255)
                                                                                                                           cAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTgg                                                                                                                                                >  1:458792/1‑289 (MQ=255)
                                                                                                                              cATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTaa                                                                                                                                                                                                      <  2:417276/232‑1 (MQ=255)
                                                                                                                              cATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTa                                                                                                                                              >  1:1271689/1‑288 (MQ=255)
                                                                                                                              cATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTa                                                                                                                                              >  1:1098183/1‑288 (MQ=255)
                                                                                                                                   gACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCg                                                                                                                                        >  1:1763975/1‑289 (MQ=255)
                                                                                                                                    aCCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGCCTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCt                                                                                                                                                                      <  2:1649759/258‑1 (MQ=255)
                                                                                                                                    aCCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAattatt                                                                                                                                                                                                                                                      >  1:495912/1‑178 (MQ=255)
                                                                                                                                    aCCAGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCt                                                                                                                                                                      >  1:1649759/1‑258 (MQ=255)
                                                                                                                                     cccGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGt                                                                                                                                      <  1:2211842/289‑1 (MQ=255)
                                                                                                                                        gCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTa                                                                                                                                                                                                                                        >  2:1333247/1‑188 (MQ=255)
                                                                                                                                        gCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGtttt                                                                                                                                   >  1:1056223/1‑289 (MQ=255)
                                                                                                                                         cATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTg                                                                                                                                  >  1:1038144/1‑289 (MQ=255)
                                                                                                                                         cATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTg                                                                                                                                  >  1:725605/1‑289 (MQ=255)
                                                                                                                                         cATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTg                                                                                                                                  >  1:1475556/1‑289 (MQ=255)
                                                                                                                                         cATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCCACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTg                                                                                                                                  >  1:1394454/1‑289 (MQ=255)
                                                                                                                                               ccAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAGCTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATc                                                                                                                             <  1:1500273/288‑1 (MQ=255)
                                                                                                                                               ccAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATc                                                                                                                             <  1:1889352/288‑1 (MQ=255)
                                                                                                                                                   caaatccACAGAACCAGAAAGCAACCCCCCAACCGAC‑TCTACACGCATCACCCCGTCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTTAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGAcat                                                                                                                        <  2:54451/284‑1 (MQ=255)
                                                                                                                                                    aaTGCAAGAGCACAAGAAAGACAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACGTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGAcatc                                                                                                                       <  2:1904959/289‑1 (MQ=255)
                                                                                                                                                        ccAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCatta                                                                                                                   >  2:715640/1‑289 (MQ=255)
                                                                                                                                                        ccAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCatta                                                                                                                   >  1:1985689/1‑289 (MQ=255)
                                                                                                                                                             gaACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTc                                                                                                                                         >  2:306711/1‑262 (MQ=255)
                                                                                                                                                               aCCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGc                                                                                                            <  2:458792/289‑1 (MQ=255)
                                                                                                                                                                ccAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGAcatc                                                                                                                       >  1:1327609/1‑277 (MQ=255)
                                                                                                                                                                    aaagaaaACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCa                                                                                                       <  2:1040954/289‑1 (MQ=255)
                                                                                                                                                                          aaCCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGtt                                                                                                 >  1:1867305/1‑289 (MQ=255)
                                                                                                                                                                           accaccAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGttt                                                                                                >  2:2052454/1‑289 (MQ=255)
                                                                                                                                                                              accaA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTa                                                                                             <  2:1867305/289‑1 (MQ=255)
                                                                                                                                                                                         tCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAtt                                                                                   <  1:1536233/289‑1 (MQ=255)
                                                                                                                                                                                         tCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTGCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAtt                                                                                   <  1:898581/289‑1 (MQ=255)
                                                                                                                                                                                         tCTACCAGCATAAACCCGGCAATAACGCCGAAAGCATACAACAAGCGACTGTCATTATTATCTTCTGGAAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAtt                                                                                   <  2:1611575/289‑1 (MQ=255)
                                                                                                                                                                                           tACCATCATAAACCCGGCAGTCACGCCGAAAGCATACAAAAAGCTACGTGCATTATGATCTTCTGGCATGTGTAAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGa                                                                                 <  2:479368/289‑1 (MQ=255)
                                                                                                                                                                                           tACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGa                                                                                 <  2:117175/289‑1 (MQ=255)
                                                                                                                                                                                                 cATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGATCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAtt                                                                                   >  1:1797614/1‑281 (MQ=255)
                                                                                                                                                                                                 cATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGATCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAtt                                                                                   <  2:1797614/281‑1 (MQ=255)
                                                                                                                                                                                                 cATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCg                                                                           >  1:67412/1‑289 (MQ=255)
                                                                                                                                                                                                   tAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTACTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGa                                                                         <  1:1553783/289‑1 (MQ=255)
                                                                                                                                                                                                      aCCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACa                                                                       >  1:285023/1‑288 (MQ=255)
                                                                                                                                                                                                          ggCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAc                                                                  <  2:1098183/289‑1 (MQ=255)
                                                                                                                                                                                                          ggCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAc                                                                  <  1:636425/289‑1 (MQ=255)
                                                                                                                                                                                                           gCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTTTGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCg                                                                           <  2:1364840/279‑1 (MQ=255)
                                                                                                                                                                                                           gCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTTTGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCg                                                                           >  1:1364840/1‑279 (MQ=255)
                                                                                                                                                                                                           gCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAcc                                                                 >  1:2056968/1‑289 (MQ=255)
                                                                                                                                                                                                           gCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAc                                                                  >  1:1720123/1‑288 (MQ=255)
                                                                                                                                                                                                             aGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCa                                                                <  2:725605/288‑1 (MQ=255)
                                                                                                                                                                                                               tcgagcggAAAGATTACACCATGCGCCGAGCATTATTTTCTTCTGGCAGGTGGGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGTTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATc                                                              <  2:971748/281‑1 (MQ=255)
                                                                                                                                                                                                                 aCGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCttt                                                           >  1:806589/1‑289 (MQ=255)
                                                                                                                                                                                                                  cGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCttt                                                           >  2:949451/1‑288 (MQ=255)
                                                                                                                                                                                                                      gAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGcaca                                                      <  1:1668512/289‑1 (MQ=255)
                                                                                                                                                                                                                                     gCGACGAGCATTATTATCTTCTGGTAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGct                                                                                                                                                                                     <  2:1325046/147‑1 (MQ=255)
                                                                                                                                                                                                                                          gaGCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGTACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCa                                  >  2:642280/1‑289 (MQ=255)
                                                                                                                                                                                                                                            gCATTATTATCTTCTGGCAGGTGTGAGGAC‑‑GGGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAACATCTTTGCACATGGTGTTCTTGTGCTCTTCa                                  >  1:618741/1‑287 (MQ=255)
                                                                                                                                                                                                                                                        tctGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAtt                                                                                   <  2:1747657/226‑1 (MQ=255)
                                                                                                                                                                                                                                                          tGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAtt                                                                                   >  2:1406223/1‑224 (MQ=255)
                                                                                                                                                                                                                                                          tGGCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGcaca                                                      <  1:950391/253‑1 (MQ=255)
                                                                                                                                                                                                                                                            gCAGGTGTGAGGAC‑‑GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGca                                                        >  2:1605946/1‑249 (MQ=255)
                                                                                                                                                                                                                                                                  gtgaggacgtgtgtgagtgtga‑‑GTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACGGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGtt           <  1:1422887/278‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tgaggacgtgtgtgagtgtga‑‑GTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGcaca                                                      <  2:232174/235‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tgaggacgtgtgtgagtgtga‑‑GTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGca                                                        >  1:698724/10‑242 (MQ=255)
                                                                                                                                                                                                                                                                           tgtgtgagtgtga‑‑GTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTc                     >  2:1496885/2‑269 (MQ=255)
                                                                                                                                                                                                                                                                           tgtgtgagtgtga‑‑GTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCAttgaaga  >  1:473662/2‑282 (MQ=255)
                                                                                                                                                                                                                                                                                 |||                                                                                                                                                                                                                                                                                         
TATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGACTAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAA‑CGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGACTGGAC‑‑GTGTGACTGTGACTGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATACTAGA  >  CP042583/647370‑647923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A