Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,472,065 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,472,0650AT98.5% 639.9 / NA 276intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (127/143);  minor base G (2/2);  total (130/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,472,0660CG98.9% 641.9 / ‑6.0 276intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (128/144);  minor base T (1/2);  total (130/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,472,0670TA99.3% 645.7 / NA 276intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (129/144);  minor base C (1/1);  total (130/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 276 total aligned reads displayed.

GTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGACTCGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGGCTCAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTAT  >  CP042583/1471785‑1472345
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                      
  ttACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCcgcg                                                                                                                                                                                                                                                                                 <  1:855922/288‑1 (MQ=255)
  ttACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCcgcg                                                                                                                                                                                                                                                                                 >  2:422414/1‑288 (MQ=255)
  ttACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATGTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCCGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCcgcg                                                                                                                                                                                                                                                                                 <  1:161742/288‑1 (MQ=255)
     ccccAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCcgcg                                                                                                                                                                                                                                                                                 <  1:938828/285‑1 (MQ=255)
         attCAGTTGCACGACTGACATAGTCATCATCGCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCTTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGtgctg                                                                                                                                                                                                                                                                         <  2:1173232/287‑1 (MQ=255)
            cAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTgctgct                                                                                                                                                                                                                                                                       <  2:713499/288‑1 (MQ=255)
                tGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAACGCCGCACGAACAATAAACCCATCCCGTAGCGGTGACCCCGCCCTGTGCCGCCCggg                                                                                                                                                                                                                                                                   >  2:1633956/1‑287 (MQ=255)
                    cgacTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGa                                                                                                                                                                                                                                                               >  2:251933/1‑288 (MQ=255)
                     gacTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACCCCGCGCTGTGCTCCCCGGTCAGAc                                                                                                                                                                                                                                                              >  2:35091/1‑288 (MQ=255)
                                 catcatCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGt                                                                                                                                                                                                                                                                   >  2:634187/1‑271 (MQ=255)
                                    catcTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAATTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATCCCGCACGCTCAATAAACTCATCACGTAGCGGTGACGCCGCGCAGTTCTGCTCGGTCAGACTCAGCACGCGTGAtgt                                                                                                                                                                                                                                              >  1:1351581/1‑289 (MQ=255)
                                     atcTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAAtgtg                                                                                                                                                                                                                                             <  1:1059555/289‑1 (MQ=255)
                                     atcTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAAtgt                                                                                                                                                                                                                                              >  1:1551808/1‑288 (MQ=255)
                                          tGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCCCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCCCGCTGTGCGGCTCGGTCAGCCCCGCGATGCGGCATGGGCgggc                                                                                                                                                                                                                                        >  2:1930519/1‑288 (MQ=255)
                                                tGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAAt                                                                                                                                                                                                                                   <  2:1151013/288‑1 (MQ=255)
                                                        gttCGCAACGCCTCATGCCGGCGGTGCAATTCCGTCATATCCCCCTTTTCGGCCGCTTCACTCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGct                                                                                                                                                                                                                          <  2:1551808/288‑1 (MQ=255)
                                                            gCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTTCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTcg                                                                                                                                                                                                                      <  2:1372513/289‑1 (MQ=255)
                                                            gCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTcg                                                                                                                                                                                                                      <  2:27403/289‑1 (MQ=255)
                                                                 gCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCa                                                                                                                                                                                                                 <  1:1112105/289‑1 (MQ=255)
                                                                            gCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATccgcctcgt                                                                                                                                                                                                       >  1:782769/1‑280 (MQ=255)
                                                                            gCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGGGCGGGTATACTCGCCGCGCTCTCGCGCCATCAGACTGaat                                                                                                                                                                                                      >  2:79838/1‑289 (MQ=255)
                                                                             cGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGa                                                                                                                                                                                                        <  1:422414/286‑1 (MQ=255)
                                                                              ggTGAAATTCCGTCAGATCGCCCTTTGCTGCCGCTTCAATCGCCCGTTGCGCCAACCCGTTGCGCAAGACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCCAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGaata                                                                                                                                                                                                     <  2:1213950/288‑1 (MQ=255)
                                                                                tGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAAtgt                                                                                                                                                                                                                                              <  2:1813352/245‑1 (MQ=255)
                                                                                tGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGaataat                                                                                                                                                                                                   <  2:856614/288‑1 (MQ=255)
                                                                                 gCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACAAGTCATCAAATGCCGCACGCCCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGa                                                                                                                                                                                                            >  1:1367277/1‑278 (MQ=255)
                                                                                    aTTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattcatt                                                                                                                                                                                              <  2:2011096/289‑1 (MQ=255)
                                                                                          tCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTa                                                                                                                                                                                         >  1:1523571/1‑288 (MQ=255)
                                                                                          tCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAg                                                                                                                                                                                        >  1:1540686/1‑289 (MQ=255)
                                                                                          tCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAACACCTTCAGTa                                                                                                                                                                                         >  1:1465747/1‑288 (MQ=255)
                                                                                              atCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGcgcg                                                                                                                                                                                    <  1:668386/289‑1 (MQ=255)
                                                                                                 aCCCGTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTa                                                                                                                                                                                 <  2:1975665/289‑1 (MQ=255)
                                                                                                  cccTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTg                                                                                                                                                                                                                              <  2:88447/243‑1 (MQ=255)
                                                                                                          tGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCtttt                                                                                                                                                                        <  1:1017537/289‑1 (MQ=255)
                                                                                                               cTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCt                                                                                                                                                                    >  1:1897832/1‑288 (MQ=255)
                                                                                                                 tCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATCATCTCTGCGCTCTCCAGCCATCAGACTGAAGAATTAATTCAGTAGCGCGTTATCCTCTTTTTTCttc                                                                                                                                                                  >  2:2237535/1‑286 (MQ=255)
                                                                                                                           gTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGTG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGcc                                                                                                                                                       <  1:2237535/289‑1 (MQ=255)
                                                                                                                            ttGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCCATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACGGAATCATTCATTCAGTAGCCCGTTATCCTCTTTTTGCTCCCTCATGATGcc                                                                                                                                                       >  2:1587065/1‑288 (MQ=255)
                                                                                                                                     ccAGTTGCGCGATTCGCGAGCGGGGTTAACGCTTTGCATCAGTTGCTCACGCTCACTATCGCTAACCTCGTCTTGGTGCAAACGCCCCCGCTAACGGG‑CAAACCAGTCAACAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGc                                                                                                                                             <  1:332614/289‑1 (MQ=255)
                                                                                                                                          tGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCAt                                                                                                                                        <  1:2089828/289‑1 (MQ=255)
                                                                                                                                          tGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCAt                                                                                                                                        <  1:1930519/289‑1 (MQ=255)
                                                                                                                                              cAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTcg                                                                                                                                                                                                                      >  1:301402/1‑207 (MQ=255)
                                                                                                                                              cAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTcg                                                                                                                                                                                                                      <  2:301402/207‑1 (MQ=255)
                                                                                                                                               aaTACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGtt                                                                                                                                   <  1:2023704/289‑1 (MQ=255)
                                                                                                                                                           gggTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCg                                                                                                                                                                 >  2:971287/1‑247 (MQ=255)
                                                                                                                                                                 aCGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGa                                                                                                                                                                                                            >  1:1612943/1‑198 (MQ=255)
                                                                                                                                                                 aCGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGa                                                                                                                                                                                                            <  2:1612943/198‑1 (MQ=255)
                                                                                                                                                                 aCGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCAGTCATGAAGCACAGTTTCTGCCGCATCCGTTCTCCATAATGAGTccacc                                                                                                                 >  2:2138153/1‑288 (MQ=255)
                                                                                                                                                                     tttGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAAc                                                                                                             <  1:1700096/289‑1 (MQ=255)
                                                                                                                                                                      ttgccacAGTTGCTGACACTCACTATCGCTAACCTCGTCTTGTTGCACACGCCCCCGAGAACGGG‑CAAACCAGTCAACAAATGCCGCACGGTCAATAAAATCATCCCGTAGCGGTGCCGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGAGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCATACTGAATAATACATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAAc                                                                                                             <  2:1465747/282‑1 (MQ=255)
                                                                                                                                                                      ttGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACaaaa                                                                                                              >  1:1429643/1‑287 (MQ=255)
                                                                                                                                                                      ttGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACaaaa                                                                                                              >  1:47765/1‑287 (MQ=255)
                                                                                                                                                                       tGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCATATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCCTTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATCATGCGTcaacaaca                                                                                                              >  2:1706460/1‑284 (MQ=255)
                                                                                                                                                                              gTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATa                                                                                                    >  1:994415/1‑289 (MQ=255)
                                                                                                                                                                                tGCTGACGCTCCCTTTCGCTGACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCCGTCCTCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTTCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTTAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAg                                                                                                   <  2:699145/288‑1 (MQ=255)
                                                                                                                                                                                         tCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACctgct                                                                                                        >  1:880709/1‑274 (MQ=255)
                                                                                                                                                                                         tCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAggg                                                                                         <  1:1387296/289‑1 (MQ=255)
                                                                                                                                                                                         ccACTATCCCAAACTCGGTCTTGTT‑CAAACCCCCCCGATAACGGGCCAAACAAGCCACAAATTGCGGCAC‑AACAAATAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGc                                                                                        <  1:1580388/288‑1 (MQ=255)
                                                                                                                                                                                          cACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGc                                                                                        <  1:250101/289‑1 (MQ=255)
                                                                                                                                                                                           aCTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGcc                                                                                       <  1:1960369/289‑1 (MQ=255)
                                                                                                                                                                                           aCTACCCCTAACCTCTTCTGGTGGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGCCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGcc                                                                                       <  2:1412096/289‑1 (MQ=255)
                                                                                                                                                                                                cGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCAtt                                                                                  <  2:487675/289‑1 (MQ=255)
                                                                                                                                                                                                cGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCAtt                                                                                  <  1:1231556/289‑1 (MQ=255)
                                                                                                                                                                                                cGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCAtt                                                                                  <  2:317892/289‑1 (MQ=255)
                                                                                                                                                                                                 gCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTc                                                                                 <  2:47765/289‑1 (MQ=255)
                                                                                                                                                                                                 gCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTc                                                                                 >  1:1168399/1‑289 (MQ=255)
                                                                                                                                                                                                     aCCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCTCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTTAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAggg                                                                             <  2:135453/289‑1 (MQ=255)
                                                                                                                                                                                                     aCCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACctgct                                                                                                        >  2:759449/1‑262 (MQ=255)
                                                                                                                                                                                                     aCCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACctgct                                                                                                        <  1:759449/262‑1 (MQ=255)
                                                                                                                                                                                                     aCCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAg                                                                                                   <  2:1302998/267‑1 (MQ=255)
                                                                                                                                                                                                       cTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattcatt                                                                                                                                                                                              <  2:391506/174‑1 (MQ=255)
                                                                                                                                                                                                       cTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAattcatt                                                                                                                                                                                              >  1:391506/1‑174 (MQ=255)
                                                                                                                                                                                                       cTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAggg                                                                                         <  1:1076522/275‑1 (MQ=255)
                                                                                                                                                                                                       cTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCa                                                                           >  1:1596350/1‑289 (MQ=255)
                                                                                                                                                                                                         cGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAAc                                                                                                             <  2:2065851/253‑1 (MQ=255)
                                                                                                                                                                                                         cGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCa                                                                                            >  2:1193549/1‑270 (MQ=255)
                                                                                                                                                                                                         cGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAgg                                                                              >  1:890386/1‑284 (MQ=255)
                                                                                                                                                                                                                     aaCGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCa                                                                                            <  1:232003/258‑1 (MQ=255)
                                                                                                                                                                                                                          ccccGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCcgcg                                                                                                                                                                                                                                                                                 >  2:937209/1‑72 (MQ=255)
                                                                                                                                                                                                                                    ggg‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATGTCt                                               >  2:589145/1‑288 (MQ=255)
                                                                                                                                                                                                                                           aCCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATTCCTGCGCCaa                                        >  2:1983944/1‑288 (MQ=255)
                                                                                                                                                                                                                                                tcatcaAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGc                                                                                                  <  2:239287/226‑1 (MQ=255)
                                                                                                                                                                                                                                                tcatcaAATGCCGCACGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCt                                   <  1:601463/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           cGCACGTTCAATAAACTCATCACGTAGCGGTGACGCCGCCCTGTTCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCcac                        <  2:1099650/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              aCGATCAATAAACTCATCACGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCCGTAAATTCCACAAc                     >  2:83595/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                            catcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAAc                                                                                                             >  2:2185512/1‑187 (MQ=255)
                                                                                                                                                                                                                                                                            catcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAAc                                                                                                             <  1:2185512/187‑1 (MQ=255)
                                                                                                                                                                                                                                                                            catcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTc                                                                                 >  1:271701/1‑215 (MQ=255)
                                                                                                                                                                                                                                                                            catcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAgg                                                                              <  2:1060169/218‑1 (MQ=255)
                                                                                                                                                                                                                                                                            catcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATGTCt                                               >  1:739374/1‑249 (MQ=255)
                                                                                                                                                                                                                                                                             atcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACctgct                                                                                                        <  1:116/191‑1 (MQ=255)
                                                                                                                                                                                                                                                                             atcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGTCGACAATGTCTGCTCCAGCCGCGGTAAATTCCACAACGCGCAGGCGGGttta      >  2:2251816/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                             atcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGa      >  1:229593/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                              tcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTCTAATCGCTGCGCTCTCGCGCCAGCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATTAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGCCCTCATTCAGGGCATCTACGTCAACAAATGGCGACAATGTCTGCCACAGACGCTGTAAATTCCCCAACGACACgg              >  2:2203333/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                              tcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCt                                                                                                                                                                    >  2:157247/1‑130 (MQ=255)
                                                                                                                                                                                                                                                                              tcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAg                                                                               <  2:1161337/215‑1 (MQ=255)
                                                                                                                                                                                                                                                                              tcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCa                                                                           >  1:45536/1‑219 (MQ=255)
                                                                                                                                                                                                                                                                              tcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGAt     >  1:2097502/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                              tcaCGTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGAt     >  1:1749038/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                  gTAGCGGTGACGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAg                                                                                                   >  2:1537443/1‑191 (MQ=255)
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                      
GTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCCTTTTCTGCCGCTTCAATCGCCCGTTGCGCCAACCAGTTGCGCAATACCAGAGCGGGGTTAACGCTTTGCATCAGTTGCTGACGCTCACTATCGCTAACCTCGTCTTGTTGCAAACGCCCCCGATAACGGG‑CAAACCAGTCATCAAATGCCGCACGATCAATAAACTCATCACGTAGCGGACTCGCCGCGCTGTGCTGCTCGGTCAGACTCAGCATGCGGAATGTGCGGGTATAATCGCTGCGCTCTCGCGCCATCAGACTGAATAATTCATTCAGTAGCGCGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGGCTCAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTAT  >  CP042583/1471785‑1472345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A