Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,472,851 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,472,8510GC99.7% 699.8 / NA 296intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (133/161);  minor base T (1/0);  total (135/161)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,472,8520CG99.0% 676.1 / NA 296intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base G (132/161);  total (135/161)
Fisher's exact test for biased strand distribution p-value = 9.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,472,8530TA99.7% 695.5 / NA 296intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (134/161);  minor base G (1/0);  total (135/161)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 296 total aligned reads displayed.

TGACTCCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCGCTCTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGGCTATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTGCTACGTAAAACCGCCCGTCCATCACCCATTCGGCTATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGACTCATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGGCTGCTTGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAG  >  CP042583/1472576‑1473131
                                                                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                                                                      
tgtgaccAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGt                                                                                                                                                                                                                                                                               <  1:283211/282‑1 (MQ=255)
tgtgaccAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGt                                                                                                                                                                                                                                                                               <  1:2005447/282‑1 (MQ=255)
tgtgaccAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGt                                                                                                                                                                                                                                                                               <  1:1970098/282‑1 (MQ=255)
         tAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCCTCAGGCCAGCa                                                                                                                                                                                                                                                                     >  1:995906/1‑288 (MQ=255)
          aaTGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCa                                                                                                                                                                                                                                                                     <  1:932610/287‑1 (MQ=255)
                  taggCAAAGTCAGCAAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGCCCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCa                                                                                                                                                                                                                                                              <  2:772326/283‑1 (MQ=255)
                   tAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGt                                                                                                                                                                                                                                                                               <  1:1385124/268‑1 (MQ=255)
                   tAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGAt                                                                                                                                                                                                                                                           >  1:1544003/1‑288 (MQ=255)
                   tAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGAt                                                                                                                                                                                                                                                           >  1:2039437/1‑288 (MQ=255)
                   tAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCAACCTTCAGAt                                                                                                                                                                                                                                                           >  1:782518/1‑288 (MQ=255)
                   tAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCAGGAACCTTTCAGAt                                                                                                                                                                                                                                                           >  1:391098/1‑288 (MQ=255)
                   tAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGGCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCCTCCAACGTAAACCCGCCCGTCCCTCACCCATTCTCCACTAACGCGTCCGCCCCGCACCCTTCAGAt                                                                                                                                                                                                                                                           >  2:1719922/1‑288 (MQ=255)
                    cacaaagcacgttAACTACCGAACCTTTACCGCATCGCGGCGGTAGAAACAAGTTTCGAAATGCCCAAAGCGCAGATGACTTGGGGCCACCCGCATAAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTAACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGc                                                                                                                                                                                                                                                         <  2:596958/277‑1 (MQ=255)
                    aGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACACATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGc                                                                                                                                                                                                                                                         <  1:913629/289‑1 (MQ=255)
                    aGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCAAAACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGc                                                                                                                                                                                                                                                         <  2:26275/289‑1 (MQ=255)
                     aacagctCAGCTAACTGACGAACCTTGTCCGACTCGCGGCGGTCATAAAAATGTTCGAAATGACCAAAGCGCCGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCCCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGACAGCACCTTTCAGATGc                                                                                                                                                                                                                                                         <  1:704058/282‑1 (MQ=255)
                      cAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCa                                                                                                                                                                                                                                                       <  1:47452/289‑1 (MQ=255)
                         aGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTc                                                                                                                                                                                                                                                    <  1:1727556/289‑1 (MQ=255)
                         aGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTc                                                                                                                                                                                                                                                    <  1:732265/289‑1 (MQ=255)
                              gCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGc                                                                                                                                                                                                                                                         <  1:1354398/279‑1 (MQ=255)
                              gCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTg                                                                                                                                                                                                                                               >  2:1639877/1‑289 (MQ=255)
                              gCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCAACACCCATTCGCGAATAAGGCGTCAGGCCATCCACTTTCAGATGCCAGTCCATTg                                                                                                                                                                                                                                               >  2:2084175/1‑289 (MQ=255)
                                     gACGAACCTGTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCCAAGCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAAAGCCCAGGTAGTGCATCGCCTCATTGGCGAGTCTTTTTCGGATGGTCGTATTTAAAACCGCTCGTCCATCGCCCATTCGCGAATAATGCGGCAGGCCAGCACCTTTGAGGTGCCGGtaaaacttctggc                                                                                                                                                                                                                                         >  2:704058/1‑276 (MQ=255)
                                           ccTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCCGGCCAGCACCTTTCAGATGCCAGTCCATTGTAATTCCCTCagc                                                                                                                                                                                                                                  >  2:927878/1‑289 (MQ=255)
                                              tttCCCGCTCGCGGCAGAAGTAAAAAGGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCAGCAGCATCGCGCCTGGCTCCGCCGTTTCCCGTTACACTGGCGAATCGCGGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCagcaag                                                                                                                                                                                                                               <  2:47112/289‑1 (MQ=255)
                                              ttgccgagccgcgGTAGTAGAAAAAAGGTTCGCAATGACCAAAGCGCTGAAGACTTGGTGCCACACGCCACAACATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGTTAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCagcaag                                                                                                                                                                                                                               <  2:1489192/280‑1 (MQ=255)
                                              tctcggactcGCGGAGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGCTGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGTTACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCagcaag                                                                                                                                                                                                                               <  1:1453959/282‑1 (MQ=255)
                                                    actcGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCCTTCGCGAATAAGGCGTCAGGCCAGCCCCTTTCAGATTCCCGTCCATTGTAGTGCCATCCGCAAGCCgttg                                                                                                                                                                                                                         >  2:970487/2‑289 (MQ=255)
                                                          ggcggTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGGTCCGTGCTCCGCCGTTTCCCGATACCCTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGcc                                                                                                                                                                                                                   <  1:1494050/289‑1 (MQ=255)
                                                                    aaaaTGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATTGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAAt                                                                                                                                                                                                          >  2:241946/1‑288 (MQ=255)
                                                                       aTGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCcg                                                                                                                                                                                                      <  1:1596854/289‑1 (MQ=255)
                                                                         gTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCCCCTTTCAGATTCCAGTCCATTGTAGTGCCATCAGCAAGCATTTGTTCGCCGAGTAAAATGccct                                                                                                                                                                                                     >  2:1930626/1‑286 (MQ=255)
                                                                           tCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGcc                                                                                                                                                                                                  <  1:1674501/289‑1 (MQ=255)
                                                                                            cAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGgcc                                                                                                                                                                                  >  1:355719/1‑288 (MQ=255)
                                                                                                  acgtGGGGCCACACGAGTCAGCGTCGCGCCTGGCTCAGCCGTTTCCCGATACACTGGCGAATCGCTGGGTGCGATACTTAACGCGCGGGTCGGCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGgcccgccc                                                                                                                                                                             <  2:1282714/284‑1 (MQ=255)
                                                                                                       gTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTTCCCTCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCCGATGGCCCCCCCCGAccc                                                                                                                                                                       >  2:663059/1‑286 (MQ=255)
                                                                                                         gCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATCCACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCg                                                                                                                                                                     <  1:970487/288‑1 (MQ=255)
                                                                                                           cacaCGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGaa                                                                                                                                                                   <  1:1050781/288‑1 (MQ=255)
                                                                                                             cacGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACtg                                                                                                                                                                >  1:1433332/1‑289 (MQ=255)
                                                                                                              acGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATTCCTCGCCCATCACCCCGTTGGCCCGCCCAGACGCCGAAAtga                                                                                                                                                               >  2:734105/1‑289 (MQ=255)
                                                                                                               cGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACtgat                                                                                                                                                              <  1:83289/289‑1 (MQ=255)
                                                                                                                  cgcgcagtCGCGCCTGGCTCAGCCGTTGCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGAc                                                                                                                                                           <  2:1807843/282‑1 (MQ=255)
                                                                                                                  cgcAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACCCTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGAc                                                                                                                                                           <  2:222858/287‑1 (MQ=255)
                                                                                                                  aTCAGCCTCCGGCCTGGCTCCGCCGTTTCCCGTTCCACTTGGGAATCACTGGTGAAGATACTTAACGGGCGGGTCGTCGGAATGCCCAGGTAATGCAGCGCCTCACTGGCGGGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTATAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGAc                                                                                                                                                           <  2:1071150/289‑1 (MQ=255)
                                                                                                                  aTCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGc                                                                                                                                                                                                                                                         <  2:113382/195‑1 (MQ=255)
                                                                                                                  aTCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGc                                                                                                                                                                                                                                                         >  1:113382/1‑195 (MQ=255)
                                                                                                                       cATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTg                                                                                                                                                      >  1:2204639/1‑289 (MQ=255)
                                                                                                                          cgcgCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCACCACCCAGTTGGCCCCCCCCGACGCCGAACCGATGACCACTGTaa                                                                                                                                                   >  2:1979808/1‑289 (MQ=255)
                                                                                                                            cgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGAc                                                                                                                                                           <  2:2133076/279‑1 (MQ=255)
                                                                                                                            cgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGAc                                                                                                                                                           >  1:2133076/1‑279 (MQ=255)
                                                                                                                            cgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTaaa                                                                                                                                                  >  1:467701/1‑288 (MQ=255)
                                                                                                                                 ggCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCTCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGAGTTCCTCGTATCCTCGAACGTAAAACCGCCCGCCCATCACCAATTCGCGAATAAGGCGTTACGCCAGCACCTTTCAGAAGCAAGTCCATCTTACGTCCATCAGCATGCAGTTGTTCGCCTAGTAAATGGCCGCGCCCATCACCCAGGTGCACCGCCCAGCAGCCGCACTTCGGACCACCGTAAAccact                                                                                                                                             >  2:1444244/1‑285 (MQ=255)
                                                                                                                                  gCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCCCCCAGTTGGCCCGCCCAGCCGCCGAACCGATGCCCACTGTACACCcgcg                                                                                                                                            >  2:1248290/1‑288 (MQ=255)
                                                                                                                                   cTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCCTCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTgcgc                                                                                                                                           <  2:425099/288‑1 (MQ=255)
                                                                                                                                       gccgTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGCTGCCAGTCCATTGTAGTGCCATCCGCAAGCAGTTGTTCGCCGATTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATAACCACTGTAAACCTGCGCCAGTg                                                                                                                                      >  1:1194136/1‑289 (MQ=255)
                                                                                                                                          gTTTACCGAGACACGGGCGAATCGCTGGTGACGATACTTACCGCGCGGGTCGTCGGAATGCCCAGAGAATGCATCGCCTCACGGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTggtg                                                                                                                                   <  2:1678806/289‑3 (MQ=255)
                                                                                                                                                    acactggcgagtCGCTGGTGAGGATACTTAACGCGCGGGTCGGCGGAATGCCCAGATAATGCATCGCCTCACTGGCGCGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAgg                                                                                                                         <  2:2161802/278‑1 (MQ=255)
                                                                                                                                                      actggcgaaTCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACTACTGTAAACCTGTGCCAGTGGTGACATACCAGGCa                                                                                                                       >  1:1890106/9‑289 (MQ=255)
                                                                                                                                                       ctggcgaaTCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGGGACATACCAGGCAg                                                                                                                      >  1:419391/8‑289 (MQ=255)
                                                                                                                                                                 gCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTcg                                                                                                            <  1:1237961/289‑1 (MQ=255)
                                                                                                                                                                 gCTAGTGACGTTCCTGAACGCGCGGGTGGTCGGAATGCCCAGGTAAGGCAGCGCCTCACGGGCGAGACTTTCGCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAACGCGTCAGGCCAGCACCCTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTcg                                                                                                            <  2:1091476/289‑1 (MQ=255)
                                                                                                                                                                 ccTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTcg                                                                                                            <  1:663059/288‑1 (MQ=255)
                                                                                                                                                                   tGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTcgcc                                                                                                          <  1:1644604/289‑1 (MQ=255)
                                                                                                                                                                   tGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTcgc                                                                                                           <  1:1282670/288‑1 (MQ=255)
                                                                                                                                                                    ggTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTcgccg                                                                                                         <  1:1697052/289‑1 (MQ=255)
                                                                                                                                                                        aCGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGCCCACTGTAAACCTCCGCCAGCGGGGACATACCAGGCAGTAGGGCTTCGCCGccc                                                                                                      >  2:1882827/1‑288 (MQ=255)
                                                                                                                                                                         cGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCa                                                                                                    <  1:1091422/289‑1 (MQ=255)
                                                                                                                                                                            tACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCagca                                                                                                                                                                                                                                 >  2:822526/1‑161 (MQ=255)
                                                                                                                                                                             aCTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTTCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGgcccgccc                                                                                                                                                                             >  2:1385797/1‑212 (MQ=255)
                                                                                                                                                                              cTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGacac                                                                                               <  1:859049/289‑1 (MQ=255)
                                                                                                                                                                               ttAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGAc                                                                                                                                                           >  2:1197891/1‑228 (MQ=255)
                                                                                                                                                                                       cgGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCCGTAGGGCTTcgc                                                                                                           >  2:2038264/1‑268 (MQ=255)
                                                                                                                                                                                       cgGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCAt                                                                                      >  1:2154314/1‑289 (MQ=255)
                                                                                                                                                                                        gggTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCAtt                                                                                     >  2:1852778/1‑289 (MQ=255)
                                                                                                                                                                                         ggTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCAttt                                                                                    >  1:634416/1‑289 (MQ=255)
                                                                                                                                                                                         ggTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCAttt                                                                                    >  1:576700/1‑289 (MQ=255)
                                                                                                                                                                                                   aTGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAagagc                                                                           <  1:1852778/288‑2 (MQ=255)
                                                                                                                                                                                                    tGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTgg                                                                                                                                                                                    >  1:1038028/1‑182 (MQ=255)
                                                                                                                                                                                                    tGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTgg                                                                                                                                                                                    <  2:1038028/182‑1 (MQ=255)
                                                                                                                                                                                                    tGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAgcga                                                                         <  2:900547/289‑4 (MQ=255)
                                                                                                                                                                                                    agcacAGACAATGCCTCGCCTCACTTGCGAGACTTTCTCGTATCGGCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCGCCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAgcga                                                                         <  2:1170579/285‑4 (MQ=255)
                                                                                                                                                                                                      cccAAATAATGACTCGCCTCACGGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCACCCGGCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCCTCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAgcgacg                                                                       <  2:1359984/289‑6 (MQ=255)
                                                                                                                                                                                                       ccgataaaTGCATCGCCTCCCGGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAgcgacg                                                                       <  2:1433332/282‑6 (MQ=255)
                                                                                                                                                                                                                gCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACCTACCAGGCAGTAGGGCTTCGCCGCCCCAGAACCCCGCTCCATTTTCAAACAgggaagattggatcc                                                              >  2:1549386/1‑275 (MQ=255)
                                                                                                                                                                                                                     gCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTa                                                                                                                                                                                                                                             <  2:1761858/108‑1 (MQ=255)
                                                                                                                                                                                                                     gCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTa                                                                                                                                                                                                                                             >  1:1761858/1‑108 (MQ=255)
                                                                                                                                                                                                                     gCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCACTGTAGTGCCATTAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCg                                                        >  2:579375/1‑289 (MQ=255)
                                                                                                                                                                                                                       cTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCgtg                                                      >  1:1422666/1‑289 (MQ=255)
                                                                                                                                                                                                                         cACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGtt                                                    >  1:24804/1‑289 (MQ=255)
                                                                                                                                                                                                                           cTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTa                                                   >  2:372241/1‑288 (MQ=255)
                                                                                                                                                                                                                                   aCTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGt                                                                                                                     <  2:214817/214‑1 (MQ=255)
                                                                                                                                                                                                                                   aCTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGt                                                                                                                     >  1:214817/1‑214 (MQ=255)
                                                                                                                                                                                                                                         tcGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTgcgc                                                                                         <  1:744659/236‑1 (MQ=255)
                                                                                                                                                                                                                                                 tcgaaCGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCaaa                             >  1:1799289/5‑288 (MQ=255)
                                                                                                                                                                                                                                                      cctAAACCCGCCCGTCAAACCCCCATTCGCGAAGAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGGCGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGcc                       <  2:85240/287‑1 (MQ=255)
                                                                                                                                                                                                                                                      cGTCAACCCGCCCGTCCGTCAACCATTCGCGAATAAGGCGTCAGGGCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGGTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGcc                       <  2:1345551/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          aaaCCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTG‑TAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCa                  <  1:925929/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                cccGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCa                                                                                                                                                                                                                                                       >  2:532657/1‑55 (MQ=255)
                                                                                                                                                                                                                                                                cccGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCa                                                                                                                                                                                                                                                       <  1:532657/55‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cGTCCATCACCCATTCGAGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCCGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCGAAATCAGCCGGGCATTATTTa           >  1:118624/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                                                                      
TGACTCCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCGCTCTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGGCTATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTGCTACGTAAAACCGCCCGTCCATCACCCATTCGGCTATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGACTCATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGGCTGCTTGGAATACTCAGCGTGTTAGCCAGTTCGGTATTATGCCAAATCAGCCGGGCATTATTTAAAGGCGTAG  >  CP042583/1472576‑1473131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A