Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,530,050 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,530,0500GC99.6% 649.7 / NA 274intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (133/140);  total (134/140)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
*CP0425831,530,0510CG99.6% 643.7 / ‑5.4 274intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (133/140);  minor base A (1/0);  total (134/140)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
*CP0425831,530,0520TA99.3% 645.4 / NA 274intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (133/138);  minor base C (0/2);  total (134/140)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

TTTGCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATGCTGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATGCTCGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGC  >  CP042583/1529771‑1530330
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
        cgctCGCGAAGAGATAGACGATGAGGTTAGTCGCCCCAAGGCGGACAAAAGCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGATGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGa                                                                                                                                                                                                                                                                          <  2:2080096/286‑1 (MQ=255)
          aTCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACACACTCCGTGGCTGCATTGGGTCAAACGAATCGAGTTCTCGCTCATCGACa                                                                                                                                                                                                                                                                        >  2:354533/1‑289 (MQ=255)
           tCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACAt                                                                                                                                                                                                                                                                       <  1:454376/289‑1 (MQ=255)
                             tGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAGTTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCCACCGGTAATGCAAttc                                                                                                                                                                                                                                                     >  2:1839541/1‑287 (MQ=255)
                             tGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATcc                                                                                                                                                                                                                                                     >  1:564879/1‑289 (MQ=255)
                                 gTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACGAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCggg                                                                                                                                                                                                                                                  <  2:749778/288‑1 (MQ=255)
                                       cgccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGt                                                                                                                                                                                                                                            <  1:2120476/288‑1 (MQ=255)
                                       cgccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGt                                                                                                                                                                                                                                            <  2:564879/288‑1 (MQ=255)
                                        gacgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTaa                                                                                                                                                                                                                                          <  1:471760/287‑1 (MQ=255)
                                         ccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAAc                                                                                                                                                                                                                                         <  1:624060/289‑1 (MQ=255)
                                               tGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCAt                                                                                                                                                                                                                                   >  1:1564106/1‑289 (MQ=255)
                                                     cAAAATCGAATGGATTATAAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACCCCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCAATATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGt                                                                                                                                                                                                                              <  1:1164851/288‑1 (MQ=255)
                                                      aaaaTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGa                                                                                                                                                                                                                            <  1:405355/289‑1 (MQ=255)
                                                        aaTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAg                                                                                                                                                                                                                          <  1:685824/289‑1 (MQ=255)
                                                          tCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTg                                                                                                                                                                                                                        <  1:2186550/289‑1 (MQ=255)
                                                          cagAAAGTATTATAAAGGGTCGGCATAAAGAAGGCGGTGGTACTGATACCGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTg                                                                                                                                                                                                                        <  2:540043/287‑1 (MQ=255)
                                                            gAAAGGATTATCCACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTCGTGCCATTGCCCAGATCGTACACCGCCGCATCGTAGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTg                                                                                                                                                                                                                        <  2:1816709/287‑1 (MQ=255)
                                                            gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGAt                                                                                                                                                                                                                      <  1:1502564/289‑1 (MQ=255)
                                                             aaaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGt                                                                                                                                                                                                                                            >  2:1401889/1‑266 (MQ=255)
                                                             aaaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATa                                                                                                                                                                                                                     >  1:1842616/1‑289 (MQ=255)
                                                                   ttATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTga                                                                                                                                                                                                                <  1:1583011/288‑1 (MQ=255)
                                                                                           tCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCggg                                                                                                                                                                                                                                                  >  2:84310/1‑230 (MQ=255)
                                                                                           tCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCttt                                                                                                                                                                                        >  1:1384130/1‑288 (MQ=255)
                                                                                           tCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCttt                                                                                                                                                                                        >  1:2256778/1‑288 (MQ=255)
                                                                                           tCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCttt                                                                                                                                                                                        >  1:790394/1‑288 (MQ=255)
                                                                                            cGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCttt                                                                                                                                                                                        <  1:1848288/287‑1 (MQ=255)
                                                                                             ggtggtACAGATAACGCTTGGGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCt                                                                                                                                                                                     <  2:2236090/289‑1 (MQ=255)
                                                                                                  tACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGa                                                                                                                                                                                                                                                                          <  2:378927/199‑1 (MQ=255)
                                                                                                  tACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGa                                                                                                                                                                                                                                                                          >  1:378927/1‑199 (MQ=255)
                                                                                                   aCTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAAt                                                                                                                                                                               <  2:1537161/289‑1 (MQ=255)
                                                                                                            gCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTCGCTGTATTGGGTCAAACGAATCGAGTTCTCTCTCATGGACATCCCCTGTCAATGCAACCCGGGTAGGGTAACCCTCATTCCGTGAAGTGATCAGTGAAGGTGTCTGAATCCCTGCGCCTTTGCTCACACTCCAGACAGt                                                                                                                                                                      >  2:1853701/1‑289 (MQ=255)
                                                                                                               ggTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGt                                                                                                                                                                                                                                            <  1:1459842/216‑1 (MQ=255)
                                                                                                               ggTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGt                                                                                                                                                                                                                                            >  2:1459842/1‑216 (MQ=255)
                                                                                                               ggTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTGGCTCACAATCCAGACAGTTT‑c                                                                                                                                                                  >  1:131112/1‑289 (MQ=255)
                                                                                                                 tGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCTGCTATCATTACCCACAAGCAAATTCGGATCAACCAACTTCGCCTGATCACTATGCCGGATGGTTGCCAACAATTTTGAGGAAATGTTACAGCCGCAACCGGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGGGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑cg                                                                                                                                                                 <  2:888089/288‑1 (MQ=255)
                                                                                                                 tGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑cgc                                                                                                                                                                >  2:2017184/1‑289 (MQ=255)
                                                                                                                    cATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTca                                                                                                                                                                                   <  1:601203/268‑1 (MQ=255)
                                                                                                                      ttGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAAt                                                                                                                                                                                                                                                       <  1:133434/198‑1 (MQ=255)
                                                                                                                      ttGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAAt                                                                                                                                                                                                                                                       >  2:133434/1‑198 (MQ=255)
                                                                                                                                 gTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAAt                                                                                                                                                <  1:1593644/289‑1 (MQ=255)
                                                                                                                                   acacCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTg                                                                                                                                                                                                                        <  2:1218770/216‑1 (MQ=255)
                                                                                                                                        gccgcATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGa                                                                                                                                                                                                      >  2:402173/1‑229 (MQ=255)
                                                                                                                                        gccgcATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTg                                                                                                                                                                                               >  1:1651522/1‑236 (MQ=255)
                                                                                                                                        gccgcATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTAc                                                                                                                                         >  1:272421/1‑289 (MQ=255)
                                                                                                                                        gccgcATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTAc                                                                                                                                         >  1:1811946/1‑289 (MQ=255)
                                                                                                                                         ccgcATCGTCGCGGGTTTCATTACCCATAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACAtctc                                                                                                                                                                                                                                                                    <  1:1191565/166‑1 (MQ=255)
                                                                                                                                         ccgcATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTc                                                                                                                                                                                                                                                                               <  1:842252/155‑1 (MQ=255)
                                                                                                                                         ccgcATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACa                                                                                                                                        >  1:537322/1‑289 (MQ=255)
                                                                                                                                         ccgcATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACa                                                                                                                                        >  1:2225834/1‑289 (MQ=255)
                                                                                                                                         ccgcATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACa                                                                                                                                        >  1:137026/1‑289 (MQ=255)
                                                                                                                                         ccgcATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAAAGAATCGAGTTCTCGCTCATGGAAATCTCCTGTCAATGCAATCCGGGGATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTTACGC‑ACAATTCTCGGAAATCAGTTACa                                                                                                                                        >  2:657950/1‑289 (MQ=255)
                                                                                                                                            cATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTg                                                                                                                                                                                                                        <  2:1095088/207‑1 (MQ=255)
                                                                                                                                                 tcgCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTg                                                                                                                                                                                                                        >  1:988000/1‑202 (MQ=255)
                                                                                                                                                  cgcgGGTTTCATTACCCACAAGCCAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTc                                                                                                                               <  2:454760/289‑1 (MQ=255)
                                                                                                                                                     gggTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCggg                                                                                                                            <  1:186617/289‑1 (MQ=255)
                                                                                                                                                           cATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCggg                                                                                                                      <  2:790394/289‑1 (MQ=255)
                                                                                                                                                           cATTACCCACAAGAAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCggg                                                                                                                      <  2:1169524/289‑1 (MQ=255)
                                                                                                                                                                     aaGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTAt                                                                                                                                                       <  2:1436021/246‑1 (MQ=255)
                                                                                                                                                                           aTTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAAtcgacgtag                                                                                                      <  1:354533/289‑9 (MQ=255)
                                                                                                                                                                           aTTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAAtcgacgtag                                                                                                      <  1:1421656/289‑9 (MQ=255)
                                                                                                                                                                                  tCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCtt                                                                                                >  1:1489786/1‑288 (MQ=255)
                                                                                                                                                                                       aaaCTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACG‑TAATCGACGTAGATGCTTTCAGCTg                                                                                         <  2:302954/289‑1 (MQ=255)
                                                                                                                                                                                         aCTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTgc                                                                                        >  1:1634427/1‑289 (MQ=255)
                                                                                                                                                                                             cGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTgcgggg                                                                                    >  1:2083956/1‑287 (MQ=255)
                                                                                                                                                                                                cTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTAc                                                                                 <  2:1997530/289‑1 (MQ=255)
                                                                                                                                                                                                cTGCTACCTTTGAAGGTTGGTTTCCAACACTTTTGGGGAAATTTTCCAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTAc                                                                                 <  2:811660/289‑1 (MQ=255)
                                                                                                                                                                                                   cTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTg                                                                                                                                                                                                                        >  1:1623638/1‑152 (MQ=255)
                                                                                                                                                                                                   cTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTg                                                                                                                                                                                                                        <  2:1623638/152‑1 (MQ=255)
                                                                                                                                                                                                             aGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGaaa                                                                    >  2:495345/1‑289 (MQ=255)
                                                                                                                                                                                                               gATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCCGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGaaaa                                                                   >  1:127341/1‑288 (MQ=255)
                                                                                                                                                                                                                  ggTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAAt                                                                                                                                                >  1:1035426/1‑208 (MQ=255)
                                                                                                                                                                                                                  ggTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAAt                                                                                                                                                <  2:1035426/208‑1 (MQ=255)
                                                                                                                                                                                                                  ggTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCg                                                                >  2:837006/1‑288 (MQ=255)
                                                                                                                                                                                                                    tttCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGa                                                                                                                                                                                                                            >  2:1378422/1‑131 (MQ=255)
                                                                                                                                                                                                                    tttCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGa                                                                                                                                                                          <  1:909874/181‑1 (MQ=255)
                                                                                                                                                                                                                    tttCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGa                                                                                                                                                                          >  2:2148256/1‑181 (MQ=255)
                                                                                                                                                                                                                    tttCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGa                                                                                                                                                                          <  1:2148256/181‑1 (MQ=255)
                                                                                                                                                                                                                    tttCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCAGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACa                                                             >  1:1198718/1‑289 (MQ=255)
                                                                                                                                                                                                                      tCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAAt                                                           >  2:1296746/1‑289 (MQ=255)
                                                                                                                                                                                                                         aaCACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGt                                                                                                                                >  2:1764671/1‑217 (MQ=255)
                                                                                                                                                                                                                         aaCACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGt                                                                                                                                <  1:1764671/217‑1 (MQ=255)
                                                                                                                                                                                                                              ttttGGGGAAATTTTACAGCCGCCACCAGCCCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCGCCGGTTAATGCAATCCGGGTATGGTAACCCCCAGTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTACGGCGCCTTTGCTCACAATCCAGACAGTTT‑CTCGACAATGATCAGAAATAAGTTACAAACGGCGCCGGCTCCGGACCGTTAATGGACGTAGAGTCTTTCTGCTTCGCCGTATCGAGTTAGAGCAAACCGTCAATTTTAt                                                      >  2:428697/1‑286 (MQ=255)
                                                                                                                                                                                                                                     gAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGCACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACg                                            >  2:165353/1‑289 (MQ=255)
                                                                                                                                                                                                                                             ccAGCCGCAACCAGTGCCTGGGCTGTATGTGGTCAAACGAATCGAGTTCTCTCTCATGGACACGTCCGCTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGa                                    <  2:60732/288‑1 (MQ=255)
                                                                                                                                                                                                                                             aCAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGa                                    <  1:1052582/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaCCATCTCCGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCa                            <  1:441092/289‑1 (MQ=255)
                                                                                                                                                                                                                                                       cgggCTCCGTGGCTGTTTTGGGTCAACCGAATCGATTTCTCGCTCATGGACATCGCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACg                          <  2:1907318/286‑1 (MQ=255)
                                                                                                                                                                                                                                                             ccGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTAc                    <  1:1727155/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              cGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTAcc                   >  1:2227509/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              cGTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTAc                    >  2:228634/1‑288 (MQ=255)
                                                                                                                                                                                                                                                               gTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGTTCCGGGACGTAAATCAACGAAATTGCTTTCACCTGCGGCGTACCCAGGGAGAGACAACCGAAAATTTTATCCTCATCACGCCAACCGATTCCTTCACGC‑CTAcccc                 >  2:1325898/1‑287 (MQ=255)
                                                                                                                                                                                                                                                               gTGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCg                  <  1:837006/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                tGGCTGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATATTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCgg                 >  2:157598/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    tGTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACt              <  1:1952820/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gTATTGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGa                >  1:1907318/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                         tGGGTCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGt        <  2:1489786/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACg                          >  1:20066/1‑267 (MQ=255)
                                                                                                                                                                                                                                                                             tCAAACGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGGACCGAGGTAGAGAAAACCGACAATTTTATACTGCTCACGGCAACCGAATGCTGCACGCACTACCGGAATTTCATGTAAt    >  2:170452/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                aaCGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACg                          >  2:2209980/1‑264 (MQ=255)
                                                                                                                                                                                                                                                                                aaCGAATCGAGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATCGACGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATgc  >  1:2027064/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
TTTGCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAGGATGGTTTCCAACACTTTTGGGGAAATTTTACAGCCGCAACCAGCTCCGTGGCTGTATTGGGTCAAACGAATGCTGTTCTCGCTCATGGACATCTCCTGTCAATGCAATCCGGGTATGGTAACCCTCATTCCGTGAAGTGATAAGTGAGAGTGTCTGAATTCCTGCGCCTTTGCTCACAATCCAGACAGTTT‑CGCGACAATTATCAGAAATAAGTTACAAACGGCGTCGGGTCCGGGACGTTAATGCTCGTAGATGCTTTCAGCTGCGGCGTACCGAGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGC  >  CP042583/1529771‑1530330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A