Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,534,872 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,534,8720GC99.4% 745.0 / NA 314intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base C (163/148);  total (164/150)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,534,8730CG99.7% 739.6 / NA 314intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (164/147);  total (164/150)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
*CP0425831,534,8740TA99.7% 750.1 / NA 314intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (164/149);  total (164/150)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 314 total aligned reads displayed.

TGGCCTACAATTCTGTACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAAGCTCACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATGCTCCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCGCTACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCGCTCGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATACTATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATACTTTTATC  >  CP042583/1534593‑1535150
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                     
tGGCCTACAATTCTGTACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACcgcg                                                                                                                                                                                                                                                                                 <  1:1134878/288‑1 (MQ=255)
tGGACTACAATTCTGTACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑GCCCGAACCgcgc                                                                                                                                                                                                                                                                                <  1:537087/289‑1 (MQ=255)
  gCCTACAATTCTGTACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAATACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAg                                                                                                                                                                                                                                                                              >  2:271888/1‑289 (MQ=255)
  gCCTACAATTCTGTACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAg                                                                                                                                                                                                                                                                              >  1:1769085/1‑289 (MQ=255)
          ttCTGTACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACcgcg                                                                                                                                                                                                                                                                                 >  1:143653/1‑278 (MQ=255)
            cTGTACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGc                                                                                                                                                                                                                                                                    >  2:528579/1‑289 (MQ=255)
               tACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGtt                                                                                                                                                                                                                                                                 >  2:1999584/1‑289 (MQ=255)
               tACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGtt                                                                                                                                                                                                                                                                 >  1:2072264/1‑289 (MQ=255)
                  tGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTTGCAATTGATGCCATCAACGAAAGATTTAATTCCCCTCAGAAATCTCTTCCACCGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACACATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCgcgc                                                                                                                                                                                                                                                                                <  1:1095327/271‑1 (MQ=255)
                    gCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑CCCCGAACCGCGCAGGCGAAAATGCGTTGGccc                                                                                                                                                                                                                                                            >  2:2117136/1‑288 (MQ=255)
                    gCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCa                                                                                                                                                                                                                                                            >  1:871865/1‑289 (MQ=255)
                         gTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGc                                                                                                                                                                                                                                                        >  1:1124177/1‑288 (MQ=255)
                         gTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCt                                                                                                                                                                                                                                                       <  1:1006235/289‑1 (MQ=255)
                             gCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCgg                                                                                                                                                                                                                                                   >  1:693960/1‑289 (MQ=255)
                              ccAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCggg                                                                                                                                                                                                                                                  >  2:824130/1‑289 (MQ=255)
                                  aTAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCgcgc                                                                                                                                                                                                                                                                                >  2:236780/1‑255 (MQ=255)
                                              aGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACcgccgac                                                                                                                                                                                                                                   >  1:581735/1‑284 (MQ=255)
                                                 ggCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACcgccgacgcg                                                                                                                                                                                                                                <  2:871865/288‑8 (MQ=255)
                                                    acaTCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAg                                                                                                                                                                                                                             <  2:927663/288‑1 (MQ=255)
                                                       tCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCaa                                                                                                                                                                                                                          >  1:1857476/1‑288 (MQ=255)
                                                                tGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAcaatacatag                                                                                                                                                                                                                >  2:1419684/1‑287 (MQ=255)
                                                                  aTGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGtat                                                                                                                                                                                                              >  2:565766/1‑289 (MQ=255)
                                                                    gCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑aa                                                                                                                                                                                                           >  2:516824/1‑289 (MQ=255)
                                                                        tCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAaag                                                                                                                                                                                                       <  1:271888/289‑1 (MQ=255)
                                                                                  aTTTAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGatcatc                                                                                                                                                                                              >  1:198968/1‑288 (MQ=255)
                                                                                   tttAATTCACCTCAGAAATCTCTTCCAACGACACCGTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCAc                                                                                                                                                                                            <  1:2201780/289‑1 (MQ=255)
                                                                                    ttAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCAcc                                                                                                                                                                                           >  1:208982/1‑289 (MQ=255)
                                                                                    ttAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCAcc                                                                                                                                                                                           >  1:2152700/1‑289 (MQ=255)
                                                                                    ttAATTCACCTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACAGACAGGTAT‑AAAAAAAAGAcaatcaac                                                                                                                                                                                           >  2:2201780/1‑287 (MQ=255)
                                                                                             cTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAg                                                                                                                                                                                  >  2:1581215/1‑289 (MQ=255)
                                                                                             cTCAGAAATCTCTTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAg                                                                                                                                                                                  >  1:976229/1‑289 (MQ=255)
                                                                                                       tctTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCTCCGACGCGAAGCAAACATACATGGAT‑AAAAAGAAGAgcatca                                                                                                                                                                                             >  1:2226265/1‑268 (MQ=255)
                                                                                                       tctTCCAACGACACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGAAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATc                                                                                                                                                                         >  2:889871/1‑288 (MQ=255)
                                                                                                         ttccaacgacACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGc                                                                                                                                                                       >  1:2182757/10‑288 (MQ=255)
                                                                                                          tccaacgacACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGccc                                                                                                                                                                     <  1:361340/281‑1 (MQ=255)
                                                                                                           ccaacgacACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCa                                                                                                                                                                    <  1:815389/282‑1 (MQ=255)
                                                                                                           ccaacgacACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCa                                                                                                                                                                    <  1:444446/282‑1 (MQ=255)
                                                                                                           ccaacgacACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCa                                                                                                                                                                    <  2:1172684/282‑1 (MQ=255)
                                                                                                           ccaacgacACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCa                                                                                                                                                                    <  1:724291/282‑1 (MQ=255)
                                                                                                              acgacACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGccc                                                                                                                                                                     >  2:1018023/5‑285 (MQ=255)
                                                                                                                          gcgTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGccc                                                                                                                                                                     <  2:447308/273‑1 (MQ=255)
                                                                                                                               tCGCTGCCAAAAATGGAAAGAACCAGCGCACAAAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAAttgaat                                                                                                                                                <  2:635755/289‑6 (MQ=255)
                                                                                                                               tCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGaa                                                                                                                                                                                                                              <  2:2075011/212‑1 (MQ=255)
                                                                                                                               tCGATGCCAAAAATGGAGAGAACCAGCGAACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAAttgaa                                                                                                                                                 <  1:2130166/289‑5 (MQ=255)
                                                                                                                               tCGAAGCCAAAAATGGAGAGACCCAGCCAAAAGAACAATAACATAACACCAAATACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAAAGCCATAGGGCGTGAGGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAAttgaat                                                                                                                                                <  2:1097770/289‑6 (MQ=255)
                                                                                                                                     ccAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGata                                                                                                                                           <  1:1581215/288‑1 (MQ=255)
                                                                                                                                                gagaACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGc                                                                                                                                                                       <  1:316542/249‑1 (MQ=255)
                                                                                                                                                          gcACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCaataata                                                                                                                      <  1:1479847/288‑1 (MQ=255)
                                                                                                                                                               gagCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCa                                                                                                                                                                                     <  2:1489648/220‑1 (MQ=255)
                                                                                                                                                                agCAATAACATAACACCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCAct                                                                                                                                                                  <  2:1886803/238‑1 (MQ=255)
                                                                                                                                                                          taacaCCAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGcc                                                                                                     >  1:461953/1‑289 (MQ=255)
                                                                                                                                                                               ccAAGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATa                                                                                                >  1:1602893/1‑289 (MQ=255)
                                                                                                                                                                                  aGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACcgccgacg                                                                                                                                                                                                                                  <  1:1898710/157‑6 (MQ=255)
                                                                                                                                                                                  aGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCa                                                                                                                                                                    <  1:1692686/218‑1 (MQ=255)
                                                                                                                                                                                  aGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAAcc                                                                                             >  2:326009/1‑289 (MQ=255)
                                                                                                                                                                                  aGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAAcc                                                                                             >  2:1222736/1‑289 (MQ=255)
                                                                                                                                                                                  aGTACCATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCATGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGaa                                                                                                                                                      <  2:253011/232‑1 (MQ=255)
                                                                                                                                                                                      ccATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACcgacg                                                                                         <  1:1222736/289‑1 (MQ=255)
                                                                                                                                                                                       cATAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACcgacga                                                                                        >  1:1179084/1‑289 (MQ=255)
                                                                                                                                                                                        aTAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCACTAATAATAAGTAAGGTGGAGCCAAATAACCGACGAg                                                                                       >  2:1608588/1‑289 (MQ=255)
                                                                                                                                                                                         tAAACACCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGCAAGGTGGAGCAAAATAACCCAAGAgg                                                                                      >  2:1854343/1‑289 (MQ=255)
                                                                                                                                                                                            acacCGTGATCGACCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAAAACGGCAATAATAATAAGTAAGGTGGAGCAAAATAACCGACGAAGaaa                                                                                   >  2:1675298/1‑289 (MQ=255)
                                                                                                                                                                                                cgtgatcgacccATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTaa                                                                                                                                   <  1:1737553/228‑1 (MQ=255)
                                                                                                                                                                                                cgtgatcgacccATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGa                                                                               >  2:1990711/10‑289 (MQ=255)
                                                                                                                                                                                                cgtgatcgacccATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGa                                                                               >  1:1801389/10‑289 (MQ=255)
                                                                                                                                                                                                cgtgatcgacccATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGa                                                                               >  2:1345499/10‑289 (MQ=255)
                                                                                                                                                                                                 gtgatcgacccATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCa                                                                                                                                                                                          >  2:1541298/9‑181 (MQ=255)
                                                                                                                                                                                                 gtgatcgacccATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGa                                                                               >  1:522509/9‑288 (MQ=255)
                                                                                                                                                                                                 gtgatcgacccATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGAt                                                                              >  2:2175519/9‑289 (MQ=255)
                                                                                                                                                                                                        acccATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGccc                                                                                                                                                                     <  2:172489/194‑1 (MQ=255)
                                                                                                                                                                                                        acccATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGccc                                                                                                                                                                     >  1:172489/2‑195 (MQ=255)
                                                                                                                                                                                                             tAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATg                                                                  <  1:1999216/289‑1 (MQ=255)
                                                                                                                                                                                                                   gtTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCa                                                                                                                                                                                     >  2:1524164/1‑168 (MQ=255)
                                                                                                                                                                                                                   gtTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCa                                                             <  1:1536090/288‑1 (MQ=255)
                                                                                                                                                                                                                    ttAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCaat                                                           >  2:496620/1‑289 (MQ=255)
                                                                                                                                                                                                                      aaataggacagaaCGCCATAGGGCGGGAAGACTGCCACGATCCGTCCGACGGCATTAACGAA‑GCCCAAACCCGGCAGGCGTAACTGCGTTGGCCAAAGCGCCGGGATATAAGCCTCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCAGCACCAGGCCATAGATTAGAATCGCCAACTCGGTAGTCTGAATTGAATAGATATAGCCTACCACGGCAATAATAATAAGTAAGGTGGAGCCAAATAAACGACGAGGAAAATGATCAATAATTAATGCCGCcataa                                                         <  2:2144902/278‑1 (MQ=255)
                                                                                                                                                                                                                       ataatGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCaataataat                                                                                                                    >  2:1783036/1‑229 (MQ=255)
                                                                                                                                                                                                                        taatGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGCAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCaataaat                                                       <  2:461953/289‑1 (MQ=255)
                                                                                                                                                                                                                           tGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAatat                                                     >  2:410395/1‑288 (MQ=255)
                                                                                                                                                                                                                            gCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATAtt                                                    >  1:1542270/1‑288 (MQ=255)
                                                                                                                                                                                                                                cAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTa                                                >  1:1256189/1‑288 (MQ=255)
                                                                                                                                                                                                                                    gCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGa                                           <  1:79652/289‑1 (MQ=255)
                                                                                                                                                                                                                                            gCGTGAAGACTGCGACGATCCGTCCGCCGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCa                                     <  1:492633/287‑1 (MQ=255)
                                                                                                                                                                                                                                             cGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCAc                                                                                                                                                                                            >  2:2040405/1‑135 (MQ=255)
                                                                                                                                                                                                                                             cGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCAc                                                                                                                                                                                            <  1:2040405/135‑1 (MQ=255)
                                                                                                                                                                                                                                             cGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAAt                                   <  1:1649758/288‑1 (MQ=255)
                                                                                                                                                                                                                                             cGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATc                                  >  1:419115/1‑289 (MQ=255)
                                                                                                                                                                                                                                             cGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATc                                  >  2:706793/1‑289 (MQ=255)
                                                                                                                                                                                                                                               tGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATc                                                                                                                                                                         >  2:76701/1‑152 (MQ=255)
                                                                                                                                                                                                                                               tGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCat                                >  2:191064/1‑289 (MQ=255)
                                                                                                                                                                                                                                                     cTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCa                                                                            <  2:1458673/239‑1 (MQ=255)
                                                                                                                                                                                                                                                      tGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGccc                                                                                                                                                                     >  1:248389/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      tGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACa                         >  2:112640/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      tGCGACGATCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACa                         >  1:1247080/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          acgaTCCGTCCGACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGAAATAAATATTCCTACAGGAGCGCCAAACATAATAACAGCgg                     >  2:173706/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          acaaaccgaccgaCGGCATTAACGAACACCG‑AACCGTGAAGGAGAAAATCAGTGGGCCAAAGCTGCGGGATATAAACCGCCAACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCTGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCg                      <  2:1958842/283‑1 (MQ=255)
                                                                                                                                                                                                                                                              cccgtccgACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCa                  <  1:1376044/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ccgACGGCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAata            <  1:735407/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        ggCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAatattgat       <  2:733929/289‑5 (MQ=255)
                                                                                                                                                                                                                                                                         gCATTAACGAA‑ACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAatattgat       <  2:1256189/288‑5 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                     
TGGCCTACAATTCTGTACTGGCATTGTAGGCCAAATAAAACACGTCAGTGGCACATCTGGCAATTGATGCCATCAACGAAAGATTTAATTCACCTCAGAAATCTCTTCCAAGCTCACCTTCCGCGTTTCGATGCCAAAAATGGAGAGAACCAGCGCACAGAGCAATAACATAACACCAAGTACCATAAACACCGTGATGCTCCCATAATGTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAA‑ACCGCTACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCGCTCGCGAAGCAAACATACATGTAT‑AAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATACTATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATACTTTTATC  >  CP042583/1534593‑1535150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A