Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,539,529 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,539,5290AT99.6% 635.9 / NA 265intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (120/144);  total (121/144)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,539,5300CG99.6% 631.7 / ‑6.2 265intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (120/144);  minor base T (1/0);  total (121/144)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
*CP0425831,539,5310TA99.2% 632.6 / NA 265intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (120/143);  minor base C (1/1);  total (121/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

TGACGACCTCAAAGTTCAATTGTGGCTCACTCGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAAACTATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAAACTATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATACTTACCATCATGTGAGAGAAATCTTCAAAACTGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAATTGCATAATGTTTATTTGTGGCATCGTTTTCCCAA  >  CP042583/1539249‑1539805
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                  
      ccTCAAAGTTCAATTGTGGCTCTGACGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCAt                                                                                                                                                                                                                                                                         <  1:1330333/288‑1 (MQ=255)
      ccTCAAAGTTCAATTGTGGCTCTGACGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCAt                                                                                                                                                                                                                                                                         <  1:251212/288‑1 (MQ=255)
      ccTCAAAGTTCAATTGTGGCTCTGACGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCAt                                                                                                                                                                                                                                                                         <  1:1419657/288‑1 (MQ=255)
       cTCAAAGTTCAATTGTGGCTCTGACGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGa                                                                                                                                                                                                                                                                       >  1:539269/1‑289 (MQ=255)
               tCAATTGTGGCTCTGACGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAg                                                                                                                                                                                                                                                                 <  2:1443731/287‑1 (MQ=255)
               tCAATTGTGGCTCTGACGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAg                                                                                                                                                                                                                                                                 <  1:210433/287‑1 (MQ=255)
                  aTTGTGGCTCTGACGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGtt                                                                                                                                                                                                                                                            <  2:943815/289‑1 (MQ=255)
                        gctctgacGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTTATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCTTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGTTCACACGAATATTTTCTTCGAAATCAAACGCGGATGCATCACGCATTAATGCATTCATACCATGAGTCCACGAGTTATGaat                                                                                                                                                                                                                                                      >  2:44654/8‑289 (MQ=255)
                          tctgacGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATGCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGaataa                                                                                                                                                                                                                                                    <  2:1775925/284‑1 (MQ=255)
                             gacGTATAAACCCCATGCTGGTTGCCGGTGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGaataa                                                                                                                                                                                                                                                    <  2:1564458/284‑1 (MQ=255)
                               cGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTcc                                                                                                                                                                                                                                               <  2:1008448/289‑1 (MQ=255)
                                gTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCa                                                                                                                                                                                                                                              <  1:1546130/289‑1 (MQ=255)
                                    aaaCCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACt                                                                                                                                                                                                                                          <  2:2126553/289‑1 (MQ=255)
                                    aaaCCCCATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACt                                                                                                                                                                                                                                          <  1:2256556/289‑1 (MQ=255)
                                        cccATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCAt                                                                                                                                                                                                                                                                         <  2:197056/254‑1 (MQ=255)
                                        cccATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCAt                                                                                                                                                                                                                                                                         >  1:197056/1‑254 (MQ=255)
                                        cccATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCg                                                                                                                                                                                                                                       >  1:1563495/1‑288 (MQ=255)
                                          cATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACACATTCAGCCGCCTGGTCAGGATCGGCATAGTGATAGCCCGCAAGCGCTTCCTCATAAACGGTTTCATTCCCGAAATGCCCTAATTCCGCCTCTACCGGAATCCCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAAAGAATATTTTCTCCGAAATCAAACGCGGAAGCCTCACGCATTAATGACTTCATACCATGAGTCAAGGCGTTATGAATAATCTCCATACTccacc                                                                                                                                                                                                                                     >  2:1277680/1‑285 (MQ=255)
                                          cATGCTGGTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCAt                                                                                                                                                                                                                                                                         >  1:2041290/1‑252 (MQ=255)
                                                 gTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATc                                                                                                                                                                                                                              >  1:145993/1‑288 (MQ=255)
                                                 gTTGCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATc                                                                                                                                                                                                                              >  1:797190/1‑288 (MQ=255)
                                                    gCCGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCAt                                                                                                                                                                                                                                                                         <  1:1368028/242‑1 (MQ=255)
                                                      ggacgccGACATCCAATGAATCACTGCCCGTACGTTCAACAAATTCAGCCGCCTGCTCCGGATCGGTATAGTGCTAGACCGCCAGCGCATCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACTCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCaag                                                                                                                                                                                                                         <  2:864824/282‑2 (MQ=255)
                                                      cGATGGCGACAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAAt                                                                                                                                                                                                                         <  2:1456617/288‑1 (MQ=255)
                                                         cgcgggcAGCCAATGAATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAg                                                                                                                                                                                                                      <  2:1591304/282‑1 (MQ=255)
                                                                         aTCACAGCCCGTTCGTTCCACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGtt                                                                                                                                                                                                     <  2:121105/289‑1 (MQ=255)
                                                                            acaGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCt                                                                                                                                                                                                  >  1:1236606/1‑289 (MQ=255)
                                                                                cccGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGaataa                                                                                                                                                                                                                                                    <  2:894803/235‑1 (MQ=255)
                                                                                cccGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCa                                                                                                                                                                                                                                              >  2:672347/1‑241 (MQ=255)
                                                                                cccGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCa                                                                                                                                                                                                                                              <  1:672347/241‑1 (MQ=255)
                                                                                cccGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGTCACCGTTGCttttt                                                                                                                                                                                              >  2:303381/1‑289 (MQ=255)
                                                                                cccGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCttttt                                                                                                                                                                                              >  1:2072226/1‑289 (MQ=255)
                                                                                 ccGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCttt                                                                                                                                                                                                <  1:1277680/286‑1 (MQ=255)
                                                                                  cGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTtgt                                                                                                                                                                                            <  2:527793/289‑1 (MQ=255)
                                                                                   gTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTtgtg                                                                                                                                                                                           <  2:2156799/289‑1 (MQ=255)
                                                                                    tACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTtgtg                                                                                                                                                                                           >  1:391103/1‑288 (MQ=255)
                                                                                          caacaaATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTAt                                                                                                                                                                                                                                                          >  1:1813079/1‑219 (MQ=255)
                                                                                          caacaaATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCAttga                                                                                                                                                                                    <  1:1603332/289‑4 (MQ=255)
                                                                                          caacaaATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATAATTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTGATTACCGGCACCGTTGCTTTTTGTGCCAttga                                                                                                                                                                                    >  2:1831445/1‑286 (MQ=255)
                                                                                          caacaaATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCCCCAGCGCTTCCTCATAAACGGTTTCTTTACCGACATGCCCCAATTTCGCCTCCACCGGAATACCCACCGGATGGGAGAAAACCACAACCCCTTTGGGTAAACGAAAATTTTTTTCGAAAACAAAAGCGGAAGCATCACGCATTAATTAATTCATACTATAAGTCCAGGCGGTATGAATAATCTCCTAAATCCGCCCATGAACCCAATAAAGTATTACCGGTACCTCTGTTTTTTTTGCCAttta                                                                                                                                                                                    >  2:1515401/1‑286 (MQ=255)
                                                                                                     gccgccTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCTGTGCTTTTTGTTCCATTGATACCATCAtgt                                                                                                                                                                         >  2:1657487/1‑289 (MQ=255)
                                                                                                                       gTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTgagag                                                                                                                                                                    >  1:1644049/1‑276 (MQ=255)
                                                                                                                       gTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCaaatg                                                                                                                                                       >  1:1916983/1‑287 (MQ=255)
                                                                                                                            gTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCAt                                                                                                                                                                                                                                                                         >  2:1868135/1‑170 (MQ=255)
                                                                                                                             tGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGaataa                                                                                                                                                                                                                                                    >  1:742901/1‑190 (MQ=255)
                                                                                                                               aTAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGTTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCa                                                                                                                                                                                                          <  2:2052705/230‑1 (MQ=255)
                                                                                                                                tAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTa                                                                                                                                               <  1:728975/288‑1 (MQ=255)
                                                                                                                                 aGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGcc                                                                                                                                                                                         >  2:746083/1‑245 (MQ=255)
                                                                                                                                  gCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGcc                                                                                                                                                                                         >  1:806613/1‑244 (MQ=255)
                                                                                                                                  gCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCaaatgag                                                                                                                                                     >  2:1166091/1‑276 (MQ=255)
                                                                                                                                  gCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTAcc                                                                                                                                             >  1:166541/1‑288 (MQ=255)
                                                                                                                                  gCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCg                                                                                                                                            >  2:472974/1‑289 (MQ=255)
                                                                                                                                  gCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCg                                                                                                                                            >  1:157021/1‑289 (MQ=255)
                                                                                                                                  gCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCg                                                                                                                                            >  2:1558308/1‑289 (MQ=255)
                                                                                                                                  gCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTCTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCg                                                                                                                                            >  2:339576/1‑289 (MQ=255)
                                                                                                                                    ccGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGAc                                                                                                                                          <  1:1162617/289‑1 (MQ=255)
                                                                                                                                       ccAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACaaa                                                                                                                                       >  2:1726502/1‑289 (MQ=255)
                                                                                                                                                     tAAACGGTTTCATTACCGAAATGCCCTAATTCCGCCTCTACCTGAAAACCCAGCGGTTGGAAGCAATCGACAGCCTCTTTTGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAg                                                                                                                         <  2:1406812/289‑1 (MQ=255)
                                                                                                                                                         cGGTTTCATTAACGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAaat                                                                                                                     <  2:1423455/289‑1 (MQ=255)
                                                                                                                                                            tttCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGtt                                                                                                                                                                                                                    >  1:609534/1‑191 (MQ=255)
                                                                                                                                                              tCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAataata                                                                                                                 >  1:1406812/1‑288 (MQ=255)
                                                                                                                                                                    ccGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCgggg                                                                                                          >  2:1253028/1‑289 (MQ=255)
                                                                                                                                                                      gACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACaa                                                                                                                                        >  1:1763326/1‑257 (MQ=255)
                                                                                                                                                                           gCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTc                                                                                                     >  2:741263/1‑287 (MQ=255)
                                                                                                                                                                            cccTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTtgt                                                                                                                                                                                            <  2:1366603/199‑1 (MQ=255)
                                                                                                                                                                            cccTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGct                                                                                                  >  2:1073613/1‑289 (MQ=255)
                                                                                                                                                                                   tCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCgg                                                                                           <  1:339436/289‑1 (MQ=255)
                                                                                                                                                                                     cGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCt                                                                                         <  2:164426/289‑1 (MQ=255)
                                                                                                                                                                                       ccTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACt                                                                                                                                                                                                                                          >  1:955108/1‑142 (MQ=255)
                                                                                                                                                                                       ccTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCAttgat                                                                                                                                                                                   <  1:1294576/197‑5 (MQ=255)
                                                                                                                                                                                       ccTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCAttgat                                                                                                                                                                                   >  2:1294576/1‑193 (MQ=255)
                                                                                                                                                                                       ccTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCaaatgagg                                                                                                                                                    >  1:1708813/1‑223 (MQ=255)
                                                                                                                                                                                       ccTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCaaatgag                                                                                                                                                     <  1:280742/227‑5 (MQ=255)
                                                                                                                                                                                       ccTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCaaatgag                                                                                                                                                     >  2:280742/1‑223 (MQ=255)
                                                                                                                                                                                       ccTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACaa                                                                                                                                        <  2:1130308/240‑1 (MQ=255)
                                                                                                                                                                                       ccTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACaa                                                                                                                                        >  1:1130308/1‑240 (MQ=255)
                                                                                                                                                                                       ccTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTg                                                                                        >  2:1063158/1‑288 (MQ=255)
                                                                                                                                                                                       ccTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGc                                                                                       >  1:1440175/1‑289 (MQ=255)
                                                                                                                                                                                           tACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTtg                                                                                                                                                                                             >  2:930593/1‑183 (MQ=255)
                                                                                                                                                                                           tACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTtg                                                                                                                                                                                             <  1:930593/183‑1 (MQ=255)
                                                                                                                                                                                           tACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCg                                                                                   >  2:568628/1‑289 (MQ=255)
                                                                                                                                                                                                  aTACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACg                                                                             <  1:979985/288‑1 (MQ=255)
                                                                                                                                                                                                   tACCCAGCGGATAGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGAAAGCATCACGCATGAATGAATTCACACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCAttg                                                                                                                                                                                     <  1:533183/183‑3 (MQ=255)
                                                                                                                                                                                                     cccAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCaaa                                                                                                                                                         <  1:1981941/209‑1 (MQ=255)
                                                                                                                                                                                                       cgtccgATGGAAGAAATGGACAGCCTCTTTGGTTAAGCGAATATTGTCTTCGAAATCAAACGCAGAAGCATCACGCATTAATGAATTCATACCATGAGTCCACGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATc                                                                       <  2:661181/284‑1 (MQ=255)
                                                                                                                                                                                                        aGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATg                                                                                                                                                                                                                                 <  2:2011639/134‑1 (MQ=255)
                                                                                                                                                                                                          cGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAAc                                                                    >  1:1007553/1‑289 (MQ=255)
                                                                                                                                                                                                             aTGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATAGTCCGACTATGATCCCACTGAGTTATTACCGGCCCCGTTGCTTTTTGTGACATGGATACcatcat                                                                                                                                                                           >  2:533183/1‑183 (MQ=255)
                                                                                                                                                                                                                       tCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTcc                                                       >  2:2213531/1‑289 (MQ=255)
                                                                                                                                                                                                                       tCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTcc                                                       <  1:485003/289‑1 (MQ=255)
                                                                                                                                                                                                                        cGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCa                                                      <  2:797190/289‑1 (MQ=255)
                                                                                                                                                                                                                           cAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGTGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACa                                                                   <  2:253855/273‑1 (MQ=255)
                                                                                                                                                                                                                                    tGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATg                                          <  1:1651473/289‑1 (MQ=255)
                                                                                                                                                                                                                                     ggTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCAT‑ACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATggg                                        <  2:1801607/289‑1 (MQ=255)
                                                                                                                                                                                                                                        tAAACGAATATTTTCTTCGAAATAAAACGCGGAAGCATCACGCATTAATGAATTCATATCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCATTACGTTAAAATGTGCa                                      >  1:2190384/1‑289 (MQ=255)
                                                                                                                                                                                                                                         aaaCGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTa                                                                                                                                >  1:525162/1‑198 (MQ=255)
                                                                                                                                                                                                                                          aaCGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAAt                                    >  1:327467/1‑289 (MQ=255)
                                                                                                                                                                                                                                              aaTATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGAGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAATTGCa                                <  2:391103/289‑1 (MQ=255)
                                                                                                                                                                                                                                               atatTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAATTGCa                                <  1:1706548/288‑1 (MQ=255)
                                                                                                                                                                                                                                                              tCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAATTGCATAATGTTTATTTGTgg                <  1:1059736/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                  
TGACGACCTCAAAGTTCAATTGTGGCTCACTCGTATAAACCCCATGCTGGTTGCCGATGGCGACAGCCAAACTATCACAGCCCGTACGTTCAACAAATTCAGCCGCCTGGTCAGGATCGGTATAGTGATAGCCCGCCAGCGCTTCCTCATAAACGGTTTCATTACCGACATGCCCTAATTCCGCCTCTACCGGAATACCCAGCGGATGGAAGAAATCGACAGCCTCTTTGGTTAAACGAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAAACTATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATACTTACCATCATGTGAGAGAAATCTTCAAAACTGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAATTGCATAATGTTTATTTGTGGCATCGTTTTCCCAA  >  CP042583/1539249‑1539805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A