Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,557,344 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,557,3440AT99.3% 689.1 / NA 297intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (140/153);  minor base G (0/2);  total (142/155)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,557,3450GC100.0% 701.3 / NA 297intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (142/155);  total (142/155)
*CP0425831,557,3460CG99.7% 700.3 / NA 297intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (141/155);  total (142/155)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

TAGCGAAGCACAGGCGGGTAGTCTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCAGCGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTAGTCTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTAT  >  CP042583/1557064‑1557625
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                       
     aaGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGcaca                                                                                                                                                                                                                                                                              >  1:557366/1‑289 (MQ=255)
     aaGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGcaca                                                                                                                                                                                                                                                                              >  1:2242895/1‑289 (MQ=255)
                ggttcacTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCggg                                                                                                                                                                                                                                                                    <  1:1235852/282‑1 (MQ=255)
                  ttcacTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTAt                                                                                                                                                                                                                                                                 >  1:402639/5‑289 (MQ=255)
                         cATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTgg                                                                                                                                                                                                                                                          >  1:171387/1‑289 (MQ=255)
                           tGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCa                                                                                                                                                                                                                                                        <  2:486235/289‑1 (MQ=255)
                                 gctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCtgt                                                                                                                                                                                                                                                  >  1:1428696/1‑289 (MQ=255)
                                   tgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGTTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGTCACAATCCGTCGGGTATTTGTTGGCAGCCTGTgg                                                                                                                                                                                                                                                >  1:229122/1‑289 (MQ=255)
                                     cccctcgaccacccagcaCCGTGTTTGCTGATCCCACTGTTCTTACATGCTGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTTGGCTCTGCTTTATGCCCTTCCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATCCTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTTCGCATCATGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTTTTTGCAGCCTGTGGgc                                                                                                                                                                                                                                              <  2:1028849/276‑1 (MQ=255)
                                        cTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGgccg                                                                                                                                                                                                                                            >  2:1940754/1‑288 (MQ=255)
                                            ccACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTTATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGGACAATCCGCCGGGTATTTGTTGGCAGCCTGGGGgccgcc                                                                                                                                                                                                                                          >  2:1999924/1‑286 (MQ=255)
                                                   agcaCCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGAt                                                                                                                                                                                                                                    <  1:523389/285‑1 (MQ=255)
                                                    gcaCCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATAATGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTa                                                                                                                                                                                                                               >  1:839472/1‑289 (MQ=255)
                                                     caCCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGCCACAACCCGTCGGGTATTTGGTGGCAGCCTGTGGGCCGCCGCGGACGGGTaa                                                                                                                                                                                                                              >  2:1153711/1‑289 (MQ=255)
                                                            tttGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTgg                                                                                                                                                                                                                                                          <  2:401105/254‑1 (MQ=255)
                                                            tttGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATaca                                                                                                                                                                                                                        <  1:1003715/288‑1 (MQ=255)
                                                                    tCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTaa                                                                                                                                                                                                               >  1:906259/1‑289 (MQ=255)
                                                                          ttttCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTa                                                                                                                                                                                                          >  1:800554/1‑288 (MQ=255)
                                                                                    tcatGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTa                                                                                                                                                                                               <  1:327843/289‑1 (MQ=255)
                                                                                    tcatGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTa                                                                                                                                                                                               <  1:1300276/289‑1 (MQ=255)
                                                                                      atGTTAAGGATAAGCGGGGGATTGCAGCGTTCGTTGCTTTGATGGGCGAAGTGGGCGCGGTTGGGCTCTGCTTTAGGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTGTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTAcc                                                                                                                                                                                             <  2:1806005/289‑1 (MQ=255)
                                                                                       tgtgAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGAt                                                                                                                                                                                                                                    >  2:133286/1‑249 (MQ=255)
                                                                                                   gCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGtt                                                                                                                                                                                >  2:646237/1‑289 (MQ=255)
                                                                                                       ggTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCa                                                                                                                                                                            <  1:646237/289‑1 (MQ=255)
                                                                                                         tATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCt                                                                                                                                                                                                                 <  2:2184026/250‑1 (MQ=255)
                                                                                                         tATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCt                                                                                                                                                                                                                 >  1:2184026/1‑250 (MQ=255)
                                                                                                         tATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCAt                                                                                                                                                                           <  1:1282657/288‑1 (MQ=255)
                                                                                                          aTTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATAc                                                                                                                                                                         <  1:604191/289‑1 (MQ=255)
                                                                                                            tGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATacac                                                                                                                                                                                                                       >  1:116942/1‑241 (MQ=255)
                                                                                                              cAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAAc                                                                                                                                                                                                              <  2:1662840/248‑1 (MQ=255)
                                                                                                              cAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACtttc                                                                                                                                                                     >  1:1076491/1‑287 (MQ=255)
                                                                                                                 cgttcgttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGGTGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGCGGGCCGCCGCTGATGAGTAACATACACGAGGCTAACGGTTACTGGTCTGTACCGCTTCTGGGGGTTGCCCAACtttccct                                                                                                                                                                  >  2:75735/1‑284 (MQ=255)
                                                                                                                 cgttcgttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGt                                                                                                                                                                                                    >  2:676646/1‑255 (MQ=255)
                                                                                                                   ttcgttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCt                                                                                                                                                                                                                 <  2:1631595/240‑1 (MQ=255)
                                                                                                                   ttcgttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACtttcact                                                                                                                                                                  >  2:1535752/1‑282 (MQ=255)
                                                                                                                   ttcgttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACtttcact                                                                                                                                                                  >  2:349597/1‑282 (MQ=255)
                                                                                                                   ttcgttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGGTGCCATACtttcact                                                                                                                                                                  >  2:563943/1‑282 (MQ=255)
                                                                                                                   ttcgttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCCGACGGGGAAACTACACGATGCTAACTTTCACTGGGTTGTACCCCTTATGGGTGTTTCCATACtttcact                                                                                                                                                                  >  2:1604958/1‑282 (MQ=255)
                                                                                                                           cTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGc                                                                                                                                                         <  1:1838202/288‑1 (MQ=255)
                                                                                                                           cTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGc                                                                                                                                                         <  1:1999924/288‑1 (MQ=255)
                                                                                                                           cTTGATGGGGGCAGGGGGGGCGGTTGGGGTCTGCTTGATGCCAGCGCACGCGATCACCTGGACTCTGCTTGTCGGTTTTGGTTCCGGCGCAACAATGAGACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGc                                                                                                                                                         <  2:1283528/288‑1 (MQ=255)
                                                                                                                                 gtgCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGC‑TTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTACATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTaa                                                                                                                                                                                                         <  1:1323037/233‑1 (MQ=255)
                                                                                                                                    cgcAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCgg                                                                                                                                                >  1:257979/1‑288 (MQ=255)
                                                                                                                                         tGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCggg                                                                                                                                                                                                                                                                    >  2:203477/1‑167 (MQ=255)
                                                                                                                                         tGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCggg                                                                                                                                                                                                                                                                    <  1:203477/167‑1 (MQ=255)
                                                                                                                                                  ttGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGgag                                                                                                                                 >  1:2209516/1‑289 (MQ=255)
                                                                                                                                                   tGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATg                                                                                                                                                                                                                   >  1:1137995/1‑206 (MQ=255)
                                                                                                                                                   tGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGgaga                                                                                                                                >  2:2118649/1‑289 (MQ=255)
                                                                                                                                                     ggCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGgagag                                                                                                                               >  1:1821352/1‑288 (MQ=255)
                                                                                                                                                               tATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGTTTCCGGAGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAAtt                                                                                                                    <  2:96300/289‑1 (MQ=255)
                                                                                                                                                               tATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAAtt                                                                                                                    <  2:2209516/289‑1 (MQ=255)
                                                                                                                                                                 tGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACTAAGAAATTc                                                                                                                   >  1:2074901/1‑288 (MQ=255)
                                                                                                                                                                       cgcACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAata                                                                                                            >  2:1144930/1‑289 (MQ=255)
                                                                                                                                                                          gacagagccccTGGACTCTGCTTGTCGGTTTTGGTTCCGGCGAAACAATGATACGTGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATc                                                                                                          <  2:1727823/279‑1 (MQ=255)
                                                                                                                                                                          ccggatgcACCTGTACGTTGTTTTTGGTTTTTGGTTCGCGAGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTGGTTATGCGCATCAGGCGGCGGAACTCTCGGGGATGGCACAATACGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATc                                                                                                          <  2:1269028/281‑1 (MQ=255)
                                                                                                                                                                          aCGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATc                                                                                                          <  1:2118649/288‑1 (MQ=255)
                                                                                                                                                                          aCGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATc                                                                                                          <  2:2056543/288‑1 (MQ=255)
                                                                                                                                                                            gcgATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCgg                                                                                                       <  1:1535752/289‑1 (MQ=255)
                                                                                                                                                                                 cACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGc                                                                                                                                                                                                                                                         >  1:438763/1‑138 (MQ=255)
                                                                                                                                                                                      ggACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTg                                                                                             <  1:626005/289‑1 (MQ=255)
                                                                                                                                                                                            tgttttgCGGTTTTCGTTCCGGCGCAACAATGATACGGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCTCATCAGGCGGCGGCACGCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTaa                                                                                       <  2:1844766/286‑1 (MQ=255)
                                                                                                                                                                                            tGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTa                                                                                                                                                                                               >  1:1980175/1‑185 (MQ=255)
                                                                                                                                                                                            tGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATc                                                                                                          <  1:1622412/270‑1 (MQ=255)
                                                                                                                                                                                            tGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATc                                                                                                          >  2:1373957/1‑270 (MQ=255)
                                                                                                                                                                                            tGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATc                                                                                                          <  1:1373957/270‑1 (MQ=255)
                                                                                                                                                                                            tGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCtat                                                                                                 >  1:741999/1‑279 (MQ=255)
                                                                                                                                                                                            tGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTa                                                                                        >  1:1639473/1‑288 (MQ=255)
                                                                                                                                                                                            tGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCGAATATCCGGTGCGATTGTGCCGTaa                                                                                       >  2:806439/1‑289 (MQ=255)
                                                                                                                                                                                              cTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGTGGCGGCACTCTCGGGGATGGCACAATCAGTCGGGCAGTTGTTGGCAGCCTGTGGGCTGCCGCTGAGGGGTTAAATACACGATGCTAAGGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATAACTTCACTACGGATGACCATTTTCGGGCTTCCAGCGCGGATGGACCAAGAAATTCGCCAATAAGCGGTGGTAGAGTGACgataca                                                                                     >  2:55087/1‑284 (MQ=255)
                                                                                                                                                                                              cTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACa                                                                                     >  1:1804959/1‑289 (MQ=255)
                                                                                                                                                                                                             tCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAg                                                                      <  1:1940754/289‑1 (MQ=255)
                                                                                                                                                                                                                ggCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGga                                                                   >  2:1985095/1‑289 (MQ=255)
                                                                                                                                                                                                                  cgcAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGgag                                                                  <  1:578116/288‑1 (MQ=255)
                                                                                                                                                                                                                  cgcAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGgag                                                                  <  2:1749154/288‑1 (MQ=255)
                                                                                                                                                                                                                     aacatggttACTGGGGTTGACGTTCAGTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCACTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGaaca                                                              <  2:256892/281‑1 (MQ=255)
                                                                                                                                                                                                                       caaTGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGaacaaa                                                            <  1:352513/289‑1 (MQ=255)
                                                                                                                                                                                                                             tACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGa                                                       <  2:363132/288‑1 (MQ=255)
                                                                                                                                                                                                                               cTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGAGTGAAAGGGAGAACAAACACGATGa                                                    >  1:2141444/1‑289 (MQ=255)
                                                                                                                                                                                                                                    gTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGaacaaacccaaagcaaatt                                               >  2:603434/1‑277 (MQ=255)
                                                                                                                                                                                                                                     ttGACGTTCCTTGGGCTGCGGGCTAGTTCTGGGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATAtt                                               <  2:1302358/288‑1 (MQ=255)
                                                                                                                                                                                                                                       gACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGc                                                                                                                                                                                                                                                         <  1:1031453/84‑1 (MQ=255)
                                                                                                                                                                                                                                       gACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGccgccg                                                                                                                                                                                                                                         >  1:1603262/1‑100 (MQ=255)
                                                                                                                                                                                                                                       gACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTg                                                                                                                                                                                                 <  2:787309/140‑1 (MQ=255)
                                                                                                                                                                                                                                       gACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTg                                                                                                                                                                                                 >  1:787309/1‑140 (MQ=255)
                                                                                                                                                                                                                                           ttCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCa                                        <  2:1076491/289‑1 (MQ=255)
                                                                                                                                                                                                                                             cATTTGTTTGCGGGCTAGTTCTGGGCATCAGGGGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCaaa                                      <  2:1195753/289‑1 (MQ=255)
                                                                                                                                                                                                                                             cATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGCGACAGGGAGAACAAACACGATGAATATTGATTCCaaa                                      >  2:1107216/1‑289 (MQ=255)
                                                                                                                                                                                                                                              aTTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAAc                                     >  1:708152/1‑289 (MQ=255)
                                                                                                                                                                                                                                               ttGGTCTGCGGGCTAGTTTGGGGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAAcg                                    <  2:2250292/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   tCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATCTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCAt                                                                               <  2:1388915/242‑1 (MQ=255)
                                                                                                                                                                                                                                                   tCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATCTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCAt                                                                               >  1:1388915/1‑242 (MQ=255)
                                                                                                                                                                                                                                                                  tgcgagtcggggggcggcACGCTCGGGGATGGAACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGaa                 <  2:1407166/277‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGaa                 <  2:319789/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    cgcATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACa               >  1:947067/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         cAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTAGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAacac                                                         <  2:431835/242‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ggcggcACTCTCGGGGTGGGCACAAGCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATgg      <  2:838443/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ggcggcACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATgg      <  2:715809/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ggcggcACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATgg      <  2:734338/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ggcggcACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATgg      <  1:1746939/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 ggcACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTAt  >  2:1450632/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                       
TAGCGAAGCACAGGCGGGTAGTCTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCAGCGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTAGTCTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTAT  >  CP042583/1557064‑1557625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A