Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,557,710 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,557,7100AT99.2% 581.1 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (142/102);  minor base C (2/0);  total (146/102)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,557,7110GC99.6% 579.3 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (145/102);  minor base A (1/0);  total (146/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,557,7120TA99.2% 588.4 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (144/102);  minor base C (2/0);  total (146/102)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GTACCACTTATGGGTGTTGCCATACTTAGTCTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGAGTACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACAGTGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAA  >  CP042583/1557434‑1557990
                                                                                                                                                                                                                                                                                    |||                                                                                                                                                                                                                                                                                      
      cTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCaaa                                                                                                                                                                                                                                                                         >  2:349184/1‑288 (MQ=255)
      cTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTAGCAAAAAAACCGATTTAGCCCTTGATGAGTGGGCTAACGAAATCACGCAGTCAACGGAACTGCGCaaa                                                                                                                                                                                                                                                                         >  2:1452204/1‑288 (MQ=255)
              tgtTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCa                                                                                                                                                                                                                                                                 >  2:2141872/1‑288 (MQ=255)
                ttGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACg                                                                                                                                                                                                                                                               >  1:377745/1‑288 (MQ=255)
                ttGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACg                                                                                                                                                                                                                                                               >  1:4512/1‑288 (MQ=255)
                  gCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCg                                                                                                                                                                                                                                                            <  1:2141872/289‑1 (MQ=255)
                  gCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCg                                                                                                                                                                                                                                                            <  1:1148838/289‑1 (MQ=255)
                        ctttcacTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCg                                                                                                                                                                                                                                                            >  2:987245/7‑283 (MQ=255)
                        ctttcacTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCg                                                                                                                                                                                                                                                            <  1:987245/277‑1 (MQ=255)
                           tcacTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGGCGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGCTGGTTATCGCATACTGGTCGACCGCCTCTGTCCTCCCGGTATCAAAAAAACCGATTTAGCCCTTGCTGAGTGGGGTCATGAAAGCACGCCGTCAACGGAACTGGGCAGAGCCTTTCGCGGccccgctgtcg                                                                                                                                                                                                                                                   >  2:31916/4‑279 (MQ=255)
                             acTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGATCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGGTCAAAGCCTTTCACTGCGacagcggcaat                                                                                                                                                                                                                                                 >  2:1309912/2‑279 (MQ=255)
                                            tttCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTcgc                                                                                                                                                                                                                                  <  1:239726/289‑1 (MQ=255)
                                            tttCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTcgc                                                                                                                                                                                                                                  <  1:721902/289‑1 (MQ=255)
                                                    tttGGGCCAGGAGAGAAAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGGGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAata                                                                                                                                                                                                                          <  2:28107/289‑1 (MQ=255)
                                                                gagaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAAc                                                                                                                                                                                                               <  1:319565/288‑1 (MQ=255)
                                                                 agaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACt                                                                                                                                                                                                              <  2:529163/288‑1 (MQ=255)
                                                                    cAAAGAAATTCGCTAATATCCGGTGCTATAGTGAAGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGgcg                                                                                                                                                                                                          <  1:2001120/289‑1 (MQ=255)
                                                                       aGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCaa                                                                                                                                                                                                       >  2:1503279/1‑289 (MQ=255)
                                                                       aGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCa                                                                                                                                                                                                        >  1:1542231/1‑288 (MQ=255)
                                                                              cGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGc                                                                                                                                                                                                <  2:1072486/289‑1 (MQ=255)
                                                                                           tGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGGTAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGGGAAGTCGTGGATTATGCAACCTTTCCCGAACAATATCTTTGCTAAATGGCCCAAACCGGAAAAGAAGGAAAGCg                                                                                                                                                                                   >  2:847677/1‑289 (MQ=255)
                                                                                           tGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGaa                                                                                                                                                                                                                <  1:1572954/260‑1 (MQ=255)
                                                                                            gCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCgg                                                                                                                                                                                  <  2:1542231/289‑1 (MQ=255)
                                                                                                taGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACCCGAGCAAGAAGGAAAGCGGCTg                                                                                                                                                                               >  1:2201946/1‑288 (MQ=255)
                                                                                                     aCGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGaa                                                                                                                                                                                                                <  2:715663/250‑1 (MQ=255)
                                                                                                     aCGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAc                                                                                                                                                                         >  2:1054557/1‑289 (MQ=255)
                                                                                                     aCGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAc                                                                                                                                                                         >  2:483079/1‑289 (MQ=255)
                                                                                                     aCGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAAAACGAGCAAGAAGGAAAGCGGCTGGGGGaa                                                                                                                                                                         >  2:1020542/1‑288 (MQ=255)
                                                                                                        tAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATc                                                                                                                                                                      >  2:764619/1‑289 (MQ=255)
                                                                                                                  tGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAAc                                                                                                                                                            >  1:1508492/1‑289 (MQ=255)
                                                                                                                  tGCGGGAAAGAGAGAACAAACACGATGAATATTCAGTGCACACGCCTTTCTGAACCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAAc                                                                                                                                                            <  2:861716/289‑1 (MQ=255)
                                                                                                                        aaaGGGAGAACAAACGCGATGAATATTCAGTCCGAACGCGTTTATGATCCGGGTGAAAAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGGGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCGTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGGGCAAGAAGGAAAGCGGCTGGCGGGCATCGCCAAAAAACAGCCGc                                                                                                                                                      <  2:1397341/289‑1 (MQ=255)
                                                                                                                            ggAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGa                                                                                                                                                   <  2:23569/288‑1 (MQ=255)
                                                                                                                             gagaACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGc                                                                                                                                                      >  2:1468042/1‑284 (MQ=255)
                                                                                                                             gagaACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGc                                                                                                                                                      <  1:1468042/284‑1 (MQ=255)
                                                                                                                              agaACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGAcc                                                                                                                                                 <  1:1909875/288‑1 (MQ=255)
                                                                                                                                 acaaacaCGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTg                                                                                                                                              <  1:1020542/288‑1 (MQ=255)
                                                                                                                                     acacGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAacac                                                                                                                                                                                                    <  1:2018010/230‑1 (MQ=255)
                                                                                                                                     acacGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGctct                                                                                                                                          >  1:1482057/1‑288 (MQ=255)
                                                                                                                                          aTGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCg                                                                                                                                                                                                                                                            >  1:2066184/1‑169 (MQ=255)
                                                                                                                                          aTGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCg                                                                                                                                                                                                                                                            <  2:2066184/169‑1 (MQ=255)
                                                                                                                                          aTGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGc                                                                                                                                                                                                <  1:675147/229‑1 (MQ=255)
                                                                                                                                          aTGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGc                                                                                                                                                                                                >  2:675147/1‑229 (MQ=255)
                                                                                                                                                   cAGTGCAAACGCGTTTATGATCCGGCTGAGAAGAGGGATGGTAATCGGTTACTGGTGGACCGGCTCTGGCAGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAaca                                                                                                                           <  2:708152/289‑1 (MQ=255)
                                                                                                                                                   cAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAaca                                                                                                                           <  1:1243204/289‑1 (MQ=255)
                                                                                                                                                   cAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAaca                                                                                                                           <  1:764619/289‑1 (MQ=255)
                                                                                                                                                       gCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAAcacca                                                                                                                        >  1:1307029/1‑288 (MQ=255)
                                                                                                                                                          aaCGAGTTTATGTACCGGCTGAACAGTGCGCTGGTTATCGCAGACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCa                                                                                                                    <  2:906259/289‑1 (MQ=255)
                                                                                                                                                              cgTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACCCAGaa                                                                                                                 >  2:1923144/1‑288 (MQ=255)
                                                                                                                                                                tttATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCCAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGCCCCTGCTATACCCAGCAAAAAACACCACCCAGAACCa                                                                                                              >  2:569285/1‑289 (MQ=255)
                                                                                                                                                                tttATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCCCTGACCCCGCTCTACCCAGCAAAAAACACCACTCAGAAcc                                                                                                               >  2:1764498/1‑288 (MQ=255)
                                                                                                                                                                tttATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAAAAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAAAACCa                                                                                                              >  2:393660/1‑289 (MQ=255)
                                                                                                                                                                 ttATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCa                                                                                                              <  1:563943/288‑1 (MQ=255)
                                                                                                                                                                 ttATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCa                                                                                                              <  1:1105464/288‑1 (MQ=255)
                                                                                                                                                                         cGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGt                                                                                                     >  1:1773103/1‑289 (MQ=255)
                                                                                                                                                                         cGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGt                                                                                                     >  1:2086638/1‑289 (MQ=255)
                                                                                                                                                                                    agCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg                                                                                             >  1:708188/1‑286 (MQ=255)
                                                                                                                                                                                     gggtaggTGATCGGATACTGGGCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg                                                                                             <  2:211538/280‑1 (MQ=255)
                                                                                                                                                                                     gCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGAc                                                                                           <  1:1687355/287‑1 (MQ=255)
                                                                                                                                                                                     gCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGAc                                                                                           <  2:1482057/287‑1 (MQ=255)
                                                                                                                                                                                      cGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAAc                                                                                                                                                                                                                                                                                 >  2:1227870/1‑104 (MQ=255)
                                                                                                                                                                                         tGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg                                                                                             >  2:930815/1‑281 (MQ=255)
                                                                                                                                                                                              aTCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCAACCAGAACCATGCGCTGGTGCTGGCCCACGGGCAACgga                                                                                 >  2:1743484/1‑286 (MQ=255)
                                                                                                                                                                                              aTCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCGCTACTCAGCACAAAACACCACGCAGAACCAGGCCCTGGTGCTGCCCCACTGGCTACgga                                                                                 >  2:1258781/1‑286 (MQ=255)
                                                                                                                                                                                                   aTACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAaca                                                                                                                           >  2:1118787/1‑241 (MQ=255)
                                                                                                                                                                                                     aCTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGa                                                                                                                                                   <  2:240078/215‑1 (MQ=255)
                                                                                                                                                                                                     aCTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGa                                                                         >  1:1867170/1‑289 (MQ=255)
                                                                                                                                                                                                      cTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCAAGCAGAACCCAGCCCTGGCGCTGGCCGACTGGCCAAGTAGCTtggcga                                                                        >  2:1512772/1‑285 (MQ=255)
                                                                                                                                                                                                         gTCGACCGCCTCTGGGCGCGCGGTATC‑AAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACAt                                                                                                                                                                       >  2:1561367/1‑190 (MQ=255)
                                                                                                                                                                                                         gTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCCGAACCATTCGCTGGTTCTGGCCGACTTGCCACGTAGCtttgtatttt                                                                     >  2:2191637/1‑281 (MQ=255)
                                                                                                                                                                                                         gTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGAtttt                                                                     >  1:555796/1‑289 (MQ=255)
                                                                                                                                                                                                         gTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGAtttt                                                                     >  2:1707779/1‑289 (MQ=255)
                                                                                                                                                                                                         gTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGAtttt                                                                     >  2:2011015/1‑289 (MQ=255)
                                                                                                                                                                                                         gTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGAttt                                                                      <  1:1415117/288‑1 (MQ=255)
                                                                                                                                                                                                             aCCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGCCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAAAACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGATACGCAGCTTGCGATTTTAGTa                                                                 >  2:401474/1‑289 (MQ=255)
                                                                                                                                                                                                                           cgcgGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGt                                                   >  1:866020/1‑289 (MQ=255)
                                                                                                                                                                                                                            gcgGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGaa                                                                                                                 >  2:1613389/1‑226 (MQ=255)
                                                                                                                                                                                                                            gcgGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGtt                                                  >  1:1197323/1‑289 (MQ=255)
                                                                                                                                                                                                                            gcgGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGt                                                   <  1:1743484/288‑1 (MQ=255)
                                                                                                                                                                                                                            gcgGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACAGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGtt                                                  >  1:270107/1‑289 (MQ=255)
                                                                                                                                                                                                                               gTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACg                                                                                                                      >  1:286187/1‑218 (MQ=255)
                                                                                                                                                                                                                               gTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACg                                                                                                                      <  2:286187/218‑1 (MQ=255)
                                                                                                                                                                                                                                          aCCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGAcc                                                                                                                                                 >  1:163650/1‑180 (MQ=255)
                                                                                                                                                                                                                                          aCCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCAc                                         >  1:1493216/1‑284 (MQ=255)
                                                                                                                                                                                                                                          aCCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGcc                                    >  1:1055128/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 tAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGTCGGATGGt                             <  1:1906849/289‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCAcgcc                       <  1:2029149/289‑1 (MQ=255)
                                                                                                                                                                                                                                                         gatgaGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCAcgccg                      >  1:653778/1‑288 (MQ=255)
                                                                                                                                                                                                                                                             acagggATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCcaca                 <  1:627626/286‑1 (MQ=255)
                                                                                                                                                                                                                                                               tGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACCGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAAt               >  2:1873021/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 ggATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAaca                                                                                                                           <  1:965724/179‑1 (MQ=255)
                                                                                                                                                                                                                                                                 ggATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAaca                                                                                                                           >  2:965724/1‑179 (MQ=255)
                                                                                                                                                                                                                                                                 ggATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCCCCAGCCGGATGGTccacccccccccaag              >  2:2029149/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                       aGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGAc                                                                                           <  1:307296/205‑1 (MQ=255)
                                                                                                                                                                                                                                                                           aTCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATtcatca   >  1:1043353/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                           aTCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCAAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATtcatca   >  1:481728/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                            tCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGCTTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCCCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCaa  >  1:929090/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                            tCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAaca                                                                                                                           >  1:1853524/1‑168 (MQ=255)
                                                                                                                                                                                                                                                                            tCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAaca                                                                                                                           <  2:1853524/168‑1 (MQ=255)
                                                                                                                                                                                                                                                                            tCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCGTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGGCACCCCGCCCCAAAGCCCATtc       >  2:627626/1‑284 (MQ=255)
                                                                                                                                                                                                                                                                                    |||                                                                                                                                                                                                                                                                                      
GTACCACTTATGGGTGTTGCCATACTTAGTCTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGAGTACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACAGTGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAA  >  CP042583/1557434‑1557990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A